ZHD3_ARATH
ID ZHD3_ARATH Reviewed; 310 AA.
AC O64722; Q8RWR4;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Zinc-finger homeodomain protein 3;
DE Short=AtZHD3;
DE AltName: Full=Homeobox protein 21;
DE Short=AtHB-21;
DE AltName: Full=Zinc finger homeodomain transcription factor 4;
GN Name=ZHD3; Synonyms=HB21, ZFHD4; OrderedLocusNames=At2g02540;
GN ORFNames=T8K22.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION.
RX PubMed=11289511; DOI=10.1023/a:1006450005648;
RA Windhoevel A., Hein I., Dabrowa R., Stockhaus J.;
RT "Characterization of a novel class of plant homeodomain proteins that bind
RT to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.";
RL Plant Mol. Biol. 45:201-214(2001).
RN [6]
RP INTERACTION WITH ZHD1; ZHD2 AND ZHD11, TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=16428600; DOI=10.1104/pp.105.070565;
RA Tan Q.K., Irish V.F.;
RT "The Arabidopsis zinc finger-homeodomain genes encode proteins with unique
RT biochemical properties that are coordinately expressed during floral
RT development.";
RL Plant Physiol. 140:1095-1108(2006).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18713354; DOI=10.1111/j.1744-7909.2008.00681.x;
RA Hu W., dePamphilis C.W., Ma H.;
RT "Phylogenetic analysis of the plant-specific zinc finger-homeobox and mini
RT zinc finger gene families.";
RL J. Integr. Plant Biol. 50:1031-1045(2008).
RN [8]
RP INTERACTION WITH HIPP30.
RX PubMed=18974936; DOI=10.1007/s11103-008-9419-0;
RA Barth O., Vogt S., Uhlemann R., Zschiesche W., Humbeck K.;
RT "Stress induced and nuclear localized HIPP26 from Arabidopsis thaliana
RT interacts via its heavy metal associated domain with the drought stress
RT related zinc finger transcription factor ATHB29.";
RL Plant Mol. Biol. 69:213-226(2009).
RN [9]
RP INTERACTION WITH MIF2 AND MIF3, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21059647; DOI=10.1074/jbc.m110.167692;
RA Hong S.-Y., Kim O.-K., Kim S.-G., Yang M.-S., Park C.-M.;
RT "Nuclear import and DNA binding of the ZHD5 transcription factor is
RT modulated by a competitive peptide inhibitor in Arabidopsis.";
RL J. Biol. Chem. 286:1659-1668(2011).
RN [10]
RP INTERACTION WITH KIN10; KIN11 AND FLZ8.
RX DOI=10.1016/j.cpb.2015.10.004;
RA Nietzsche M., Landgraf R., Tohge T., Boernke F.;
RT "A protein-protein interaction network linking the energy-sensor kinase
RT SnRK1 to multiple signaling pathways in Arabidopsis thaliana.";
RL Curr. Plant Biol. 5:36-44(2016).
CC -!- FUNCTION: Putative transcription factor. {ECO:0000250}.
CC -!- SUBUNIT: Homo- and heterodimer with other ZFHD proteins (By
CC similarity). Interacts with MIF2 and MIF3; these interactions prevent
CC nuclear localization and DNA-binding to inhibit transcription
CC regulation activity. Binds to ZHD1, ZHD2 and ZHD11. Interacts with
CC HIPP30 (PubMed:18974936). Interacts with KIN10, KIN11 and FLZ8
CC (Ref.10). {ECO:0000250, ECO:0000269|PubMed:16428600,
CC ECO:0000269|PubMed:18974936, ECO:0000269|PubMed:21059647,
CC ECO:0000269|Ref.10}.
CC -!- INTERACTION:
CC O64722; Q39011: ASK7; NbExp=3; IntAct=EBI-1806244, EBI-1798250;
CC O64722; C0SUZ3: At1g35490; NbExp=3; IntAct=EBI-1806244, EBI-15192249;
CC O64722; Q9FG01: ATO; NbExp=3; IntAct=EBI-1806244, EBI-4475455;
CC O64722; Q9M128: BHLH57; NbExp=3; IntAct=EBI-1806244, EBI-15195499;
CC O64722; Q8RU59: bZIP48; NbExp=4; IntAct=EBI-1806244, EBI-943044;
CC O64722; Q94ID6: ERF12; NbExp=3; IntAct=EBI-1806244, EBI-4446727;
CC O64722; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-1806244, EBI-2000137;
CC O64722; Q9FE67: ERF9; NbExp=3; IntAct=EBI-1806244, EBI-4431933;
CC O64722; Q940G6: GID1C; NbExp=3; IntAct=EBI-1806244, EBI-963794;
CC O64722; O24407: IAA16; NbExp=3; IntAct=EBI-1806244, EBI-632231;
CC O64722; O24409: IAA19; NbExp=3; IntAct=EBI-1806244, EBI-632257;
CC O64722; O24410: IAA20; NbExp=3; IntAct=EBI-1806244, EBI-632272;
CC O64722; Q93WC4: IAA29; NbExp=3; IntAct=EBI-1806244, EBI-3946697;
CC O64722; Q9C5X0: IAA34; NbExp=4; IntAct=EBI-1806244, EBI-3946459;
CC O64722; Q4PSE2: NFYC8; NbExp=3; IntAct=EBI-1806244, EBI-15191571;
CC O64722; Q93WV6: WRKY68; NbExp=3; IntAct=EBI-1806244, EBI-25512440;
CC O64722; Q9FIW9: ZHD10; NbExp=4; IntAct=EBI-1806244, EBI-1806298;
CC O64722; Q9FRL5: ZHD5; NbExp=4; IntAct=EBI-1806244, EBI-1806169;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC Note=Interactions with MIF proteins prevent nuclear subcellular
CC location and leads to a scattered repartition throughout the cytoplasm.
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers and inflorescence.
CC {ECO:0000269|PubMed:16428600}.
CC -!- DOMAIN: The homeodomain differs form the typical one by having namely 4
CC instead of 3 extra amino acids inserted in the loop between helix 1 and
CC helix 2.
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DR EMBL; AC004136; AAC18932.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05592.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM63181.1; -; Genomic_DNA.
DR EMBL; AY091515; AAM10791.1; -; mRNA.
DR EMBL; AY773834; AAV63863.1; -; mRNA.
DR PIR; T00609; T00609.
DR RefSeq; NP_001318182.1; NM_001335126.1.
DR RefSeq; NP_178358.1; NM_126310.3.
DR AlphaFoldDB; O64722; -.
DR SMR; O64722; -.
DR BioGRID; 186; 79.
DR IntAct; O64722; 83.
DR STRING; 3702.AT2G02540.1; -.
DR iPTMnet; O64722; -.
DR PaxDb; O64722; -.
DR PRIDE; O64722; -.
DR ProteomicsDB; 232323; -.
DR EnsemblPlants; AT2G02540.1; AT2G02540.1; AT2G02540.
DR EnsemblPlants; AT2G02540.2; AT2G02540.2; AT2G02540.
DR GeneID; 814784; -.
DR Gramene; AT2G02540.1; AT2G02540.1; AT2G02540.
DR Gramene; AT2G02540.2; AT2G02540.2; AT2G02540.
DR KEGG; ath:AT2G02540; -.
DR Araport; AT2G02540; -.
DR TAIR; locus:2065304; AT2G02540.
DR eggNOG; ENOG502QSB4; Eukaryota.
DR HOGENOM; CLU_039237_2_1_1; -.
DR InParanoid; O64722; -.
DR OMA; RHHNPAN; -.
DR OrthoDB; 1302249at2759; -.
DR PhylomeDB; O64722; -.
DR PRO; PR:O64722; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64722; baseline and differential.
DR Genevisible; O64722; AT.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR006455; Homeodomain_ZF_HD.
DR InterPro; IPR006456; ZF_HD_homeobox_Cys/His_dimer.
DR Pfam; PF04770; ZF-HD_dimer; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01565; homeo_ZF_HD; 1.
DR TIGRFAMs; TIGR01566; ZF_HD_prot_N; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51523; ZF_HD_DIMER; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..310
FT /note="Zinc-finger homeodomain protein 3"
FT /id="PRO_0000426017"
FT ZN_FING 87..136
FT /note="ZF-HD dimerization-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00856"
FT DNA_BIND 222..285
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 274
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 310 AA; 34716 MW; BEE7142651C15321 CRC64;
MEIASQEDPI PINTSYGNSG GGHGNMNHHH HANSAPSSLN ITTSNPLLVS SNSNGLGKNH
DHSHHHHVGY NIMVTNIKKE KPVVIKYKEC LKNHAATMGG NAIDGCGEFM PSGEEGSIEA
LTCSVCNCHR NFHRRETEGE EKTFFSPYLN HHQPPPQQRK LMFHHKMIKS PLPQQMIMPI
GVTTAGSNSE SEDLMEEEGG GSLTFRQPPP PPSPYSYGHN QKKRFRTKFT QEQKEKMISF
AERVGWKIQR QEESVVQQLC QEIGIRRRVL KVWMHNNKQN LSKKSNNVSN NVDLSAGNND
ITENLASTNP
