ZHD5_ARATH
ID ZHD5_ARATH Reviewed; 309 AA.
AC Q9FRL5;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Zinc-finger homeodomain protein 5;
DE Short=AtZHD5;
DE AltName: Full=Homeobox protein 33;
DE Short=AtHB-33;
GN Name=ZHD5; Synonyms=HB33; OrderedLocusNames=At1g75240; ORFNames=F22H5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INTERACTION WITH ZHD1; ZHD2; ZHD4; ZHD10 AND ZHD11, TISSUE
RP SPECIFICITY, MUTAGENESIS OF MET-292, AND GENE FAMILY.
RX PubMed=16428600; DOI=10.1104/pp.105.070565;
RA Tan Q.K., Irish V.F.;
RT "The Arabidopsis zinc finger-homeodomain genes encode proteins with unique
RT biochemical properties that are coordinately expressed during floral
RT development.";
RL Plant Physiol. 140:1095-1108(2006).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18713354; DOI=10.1111/j.1744-7909.2008.00681.x;
RA Hu W., dePamphilis C.W., Ma H.;
RT "Phylogenetic analysis of the plant-specific zinc finger-homeobox and mini
RT zinc finger gene families.";
RL J. Integr. Plant Biol. 50:1031-1045(2008).
RN [8]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, HOMODIMER, INTERACTION WITH
RP MIF1; MIF2 AND MIF3, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21059647; DOI=10.1074/jbc.m110.167692;
RA Hong S.-Y., Kim O.-K., Kim S.-G., Yang M.-S., Park C.-M.;
RT "Nuclear import and DNA binding of the ZHD5 transcription factor is
RT modulated by a competitive peptide inhibitor in Arabidopsis.";
RL J. Biol. Chem. 286:1659-1668(2011).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ABSCISIC ACID AND ARF2.
RX PubMed=21779177; DOI=10.1371/journal.pgen.1002172;
RA Wang L., Hua D., He J., Duan Y., Chen Z., Hong X., Gong Z.;
RT "Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33
RT mediate abscisic acid response in Arabidopsis.";
RL PLoS Genet. 7:E1002172-E1002172(2011).
CC -!- FUNCTION: Putative transcription factor. Binds DNA at 5'-ATTA-3'
CC consensus promoter regions. Regulates floral architecture and leaf
CC development. Regulators in the abscisic acid (ABA) signal pathway that
CC confers sensitivity to ABA in an ARF2-dependent manner.
CC {ECO:0000269|PubMed:16428600, ECO:0000269|PubMed:21059647,
CC ECO:0000269|PubMed:21779177}.
CC -!- SUBUNIT: Homo- and heterodimer with other ZFHD proteins. Interacts with
CC MIF1, MIF2 and MIF3; these interactions prevent nuclear localization
CC and DNA-binding to inhibit transcription regulation activity. Binds to
CC ZHD1, ZHD2, ZHD4, ZHD10 and ZHD11. {ECO:0000269|PubMed:16428600,
CC ECO:0000269|PubMed:21059647}.
CC -!- INTERACTION:
CC Q9FRL5; Q9FIW9: ZHD10; NbExp=4; IntAct=EBI-1806169, EBI-1806298;
CC Q9FRL5; O64722: ZHD3; NbExp=4; IntAct=EBI-1806169, EBI-1806244;
CC Q9FRL5; Q9SVL0: ZHD7; NbExp=3; IntAct=EBI-1806169, EBI-1806382;
CC Q9FRL5; Q9LHF0: ZHD9; NbExp=3; IntAct=EBI-1806169, EBI-1806440;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21059647}.
CC Note=Interactions with MIF proteins prevent nuclear subcellular
CC location and leads to a scattered repartition throughout the cytoplasm.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers and inflorescence.
CC {ECO:0000269|PubMed:16428600}.
CC -!- INDUCTION: Repressed by abscisic acid (ABA) and ARF2.
CC {ECO:0000269|PubMed:21779177}.
CC -!- DOMAIN: The homeodomain differs form the typical one by having namely 4
CC instead of 3 extra amino acids inserted in the loop between helix 1 and
CC helix 2.
CC -!- DISRUPTION PHENOTYPE: Increased resistance to abscisic acid (ABA) in
CC the seed germination and primary root growth.
CC {ECO:0000269|PubMed:21779177}.
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DR EMBL; AC025814; AAG12686.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35692.1; -; Genomic_DNA.
DR EMBL; AK229038; BAF00922.1; -; mRNA.
DR EMBL; BT028894; ABI49441.1; -; mRNA.
DR EMBL; AY084462; AAM61034.1; -; mRNA.
DR PIR; G96782; G96782.
DR RefSeq; NP_565106.1; NM_106180.4.
DR AlphaFoldDB; Q9FRL5; -.
DR SMR; Q9FRL5; -.
DR BioGRID; 29080; 11.
DR IntAct; Q9FRL5; 10.
DR STRING; 3702.AT1G75240.1; -.
DR iPTMnet; Q9FRL5; -.
DR PaxDb; Q9FRL5; -.
DR PRIDE; Q9FRL5; -.
DR ProteomicsDB; 242976; -.
DR EnsemblPlants; AT1G75240.1; AT1G75240.1; AT1G75240.
DR GeneID; 843861; -.
DR Gramene; AT1G75240.1; AT1G75240.1; AT1G75240.
DR KEGG; ath:AT1G75240; -.
DR Araport; AT1G75240; -.
DR TAIR; locus:2025121; AT1G75240.
DR eggNOG; ENOG502QZSB; Eukaryota.
DR HOGENOM; CLU_039237_2_0_1; -.
DR InParanoid; Q9FRL5; -.
DR OMA; NTRVSCN; -.
DR OrthoDB; 1185104at2759; -.
DR PhylomeDB; Q9FRL5; -.
DR PRO; PR:Q9FRL5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FRL5; baseline and differential.
DR Genevisible; Q9FRL5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006455; Homeodomain_ZF_HD.
DR InterPro; IPR006456; ZF_HD_homeobox_Cys/His_dimer.
DR Pfam; PF04770; ZF-HD_dimer; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01565; homeo_ZF_HD; 1.
DR TIGRFAMs; TIGR01566; ZF_HD_prot_N; 1.
DR PROSITE; PS51523; ZF_HD_DIMER; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; DNA-binding; Homeobox; Metal-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..309
FT /note="Zinc-finger homeodomain protein 5"
FT /id="PRO_0000426019"
FT ZN_FING 76..125
FT /note="ZF-HD dimerization-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00856"
FT DNA_BIND 240..303
FT /note="Homeobox"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 292
FT /note="Required for DNA-binding"
FT MUTAGEN 292
FT /note="M->A,F: Impaired DNA-binding."
FT /evidence="ECO:0000269|PubMed:16428600"
SQ SEQUENCE 309 AA; 33893 MW; 4B4CE843015B51CA CRC64;
MDMRSHEMIE RRREDNGNNN GGVVISNIIS TNIDDNCNGN NNNTRVSCNS QTLDHHQSKS
PSSFSISAAA KPTVRYRECL KNHAASVGGS VHDGCGEFMP SGEEGTIEAL RCAACDCHRN
FHRKEMDGVG SSDLISHHRH HHYHHNQYGG GGGRRPPPPN MMLNPLMLPP PPNYQPIHHH
KYGMSPPGGG GMVTPMSVAY GGGGGGAESS SEDLNLYGQS SGEGAGAAAG QMAFSMSSSK
KRFRTKFTTD QKERMMDFAE KLGWRMNKQD EEELKRFCGE IGVKRQVFKV WMHNNKNNAK
KPPTPTTTL
