ZHD8_ARATH
ID ZHD8_ARATH Reviewed; 271 AA.
AC Q9LXG0;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Zinc-finger homeodomain protein 8;
DE Short=AtZHD8;
DE AltName: Full=Homeobox protein 30;
DE Short=AtHB-30;
DE AltName: Full=Zinc finger homeodomain transcriptional factor ZFHD3;
GN Name=ZHD8; Synonyms=HB30, ZFHD3; OrderedLocusNames=At5g15210;
GN ORFNames=F8M21.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH ZHD1; ZHD2; ZHD4; ZHD10 AND ZHD11, TISSUE SPECIFICITY, AND
RP GENE FAMILY.
RX PubMed=16428600; DOI=10.1104/pp.105.070565;
RA Tan Q.K., Irish V.F.;
RT "The Arabidopsis zinc finger-homeodomain genes encode proteins with unique
RT biochemical properties that are coordinately expressed during floral
RT development.";
RL Plant Physiol. 140:1095-1108(2006).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18713354; DOI=10.1111/j.1744-7909.2008.00681.x;
RA Hu W., dePamphilis C.W., Ma H.;
RT "Phylogenetic analysis of the plant-specific zinc finger-homeobox and mini
RT zinc finger gene families.";
RL J. Integr. Plant Biol. 50:1031-1045(2008).
RN [6]
RP INTERACTION WITH HIPP30.
RX PubMed=18974936; DOI=10.1007/s11103-008-9419-0;
RA Barth O., Vogt S., Uhlemann R., Zschiesche W., Humbeck K.;
RT "Stress induced and nuclear localized HIPP26 from Arabidopsis thaliana
RT interacts via its heavy metal associated domain with the drought stress
RT related zinc finger transcription factor ATHB29.";
RL Plant Mol. Biol. 69:213-226(2009).
RN [7]
RP INTERACTION WITH MIF1; MIF2 AND MIF3, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21059647; DOI=10.1074/jbc.m110.167692;
RA Hong S.-Y., Kim O.-K., Kim S.-G., Yang M.-S., Park C.-M.;
RT "Nuclear import and DNA binding of the ZHD5 transcription factor is
RT modulated by a competitive peptide inhibitor in Arabidopsis.";
RL J. Biol. Chem. 286:1659-1668(2011).
CC -!- FUNCTION: Putative transcription factor.
CC -!- SUBUNIT: Homo- and heterodimer with other ZFHD proteins (By
CC similarity). Interacts with MIF1, MIF2 and MIF3; these interactions
CC prevent nuclear localization and DNA-binding to inhibit transcription
CC regulation activity. Binds to ZHD1, ZHD2, ZHD4, ZHD10 and ZHD11.
CC Interacts with HIPP30 (PubMed:18974936). {ECO:0000250,
CC ECO:0000269|PubMed:16428600, ECO:0000269|PubMed:18974936,
CC ECO:0000269|PubMed:21059647}.
CC -!- INTERACTION:
CC Q9LXG0; Q9LJW5: MIF2; NbExp=3; IntAct=EBI-1806405, EBI-15191779;
CC Q9LXG0; F4K5X6: RVE2; NbExp=3; IntAct=EBI-1806405, EBI-15194007;
CC Q9LXG0; Q9FKP8: ZHD1; NbExp=4; IntAct=EBI-1806405, EBI-766685;
CC Q9LXG0; Q9SB61: ZHD1; NbExp=3; IntAct=EBI-1806405, EBI-1806256;
CC Q9LXG0; Q9FIW9: ZHD10; NbExp=7; IntAct=EBI-1806405, EBI-1806298;
CC Q9LXG0; Q9SVL0: ZHD7; NbExp=4; IntAct=EBI-1806405, EBI-1806382;
CC Q9LXG0; Q9LHF0: ZHD9; NbExp=3; IntAct=EBI-1806405, EBI-1806440;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Note=Interactions with MIF
CC proteins prevent nuclear subcellular location and leads to a scattered
CC repartition throughout the cytoplasm. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers and inflorescence.
CC {ECO:0000269|PubMed:16428600}.
CC -!- DOMAIN: The homeodomain differs form the typical one by having namely 4
CC instead of 3 extra amino acids inserted in the loop between helix 1 and
CC helix 2.
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DR EMBL; AL353993; CAB89331.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92130.1; -; Genomic_DNA.
DR EMBL; BT012195; AAS76682.1; -; mRNA.
DR PIR; T49956; T49956.
DR RefSeq; NP_197025.1; NM_121525.4.
DR AlphaFoldDB; Q9LXG0; -.
DR SMR; Q9LXG0; -.
DR BioGRID; 16650; 57.
DR IntAct; Q9LXG0; 54.
DR STRING; 3702.AT5G15210.1; -.
DR iPTMnet; Q9LXG0; -.
DR PaxDb; Q9LXG0; -.
DR PRIDE; Q9LXG0; -.
DR ProteomicsDB; 232309; -.
DR EnsemblPlants; AT5G15210.1; AT5G15210.1; AT5G15210.
DR GeneID; 831373; -.
DR Gramene; AT5G15210.1; AT5G15210.1; AT5G15210.
DR KEGG; ath:AT5G15210; -.
DR Araport; AT5G15210; -.
DR TAIR; locus:2150871; AT5G15210.
DR eggNOG; ENOG502QWG3; Eukaryota.
DR HOGENOM; CLU_039237_0_0_1; -.
DR InParanoid; Q9LXG0; -.
DR OMA; FHRREED; -.
DR OrthoDB; 1442390at2759; -.
DR PhylomeDB; Q9LXG0; -.
DR PRO; PR:Q9LXG0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LXG0; baseline and differential.
DR Genevisible; Q9LXG0; AT.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006455; Homeodomain_ZF_HD.
DR InterPro; IPR006456; ZF_HD_homeobox_Cys/His_dimer.
DR Pfam; PF04770; ZF-HD_dimer; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01565; homeo_ZF_HD; 1.
DR TIGRFAMs; TIGR01566; ZF_HD_prot_N; 1.
DR PROSITE; PS51523; ZF_HD_DIMER; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..271
FT /note="Zinc-finger homeodomain protein 8"
FT /id="PRO_0000426022"
FT ZN_FING 56..107
FT /note="ZF-HD dimerization-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00856"
FT DNA_BIND 179..242
FT /note="Homeobox"
FT REGION 125..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 231
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250"
FT MOD_RES 16
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LHF0"
SQ SEQUENCE 271 AA; 29382 MW; 59F908DAD70DED76 CRC64;
MDVIATTTTI VSDLDSRQPE IEAPIRIQPA KPISFSNGKR CHHHHLASEA VAVATYKECL
KNHAAGIGGH ALDGCGEFMP SPSFNSNDPA SLTCAACGCH RNFHRREEDP SSLSAIVPAI
EFRPHNRHQL PPPPPPHLAG IRSPDDDDSA SPPPISSSYM LLALSGGRGG ANTAVPMSRK
RFRTKFSQYQ KEKMFEFSER VGWRMPKADD VVVKEFCREI GVDKSVFKVW MHNNKISGRS
GARRANGGVV VGGVGDSRQS VVPTNGSFSS T
