ZIC1_XENLA
ID ZIC1_XENLA Reviewed; 443 AA.
AC O73689; O57461;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Zinc finger protein ZIC 1;
DE Short=XZic1;
DE Short=XlZic1;
DE AltName: Full=ODD-paired-like;
DE Short=Xopl;
DE AltName: Full=ZIC-related protein 1;
DE Short=ZIC-r1;
DE AltName: Full=Zinc finger protein of the cerebellum 1;
GN Name=zic1; Synonyms=opl;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC TISSUE=Neurula;
RX PubMed=9435279; DOI=10.1242/dev.125.4.579;
RA Mizuseki K., Kishi M., Matsui M., Nakanishi S., Sasai Y.;
RT "Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have
RT distinct activities in the initiation of neural induction.";
RL Development 125:579-587(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=9655809; DOI=10.1242/dev.125.15.2867;
RA Kuo J.S., Patel M., Gamse J., Merzdorf C., Liu X., Apekin V., Sive H.;
RT "Opl: a zinc finger protein that regulates neural determination and
RT patterning in Xenopus.";
RL Development 125:2867-2882(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Neurula;
RX PubMed=9739105; DOI=10.1016/s0925-4773(98)00073-2;
RA Nakata K., Nagai T., Aruga J., Mikoshiba K.;
RT "Xenopus Zic family and its role in neural and neural crest development.";
RL Mech. Dev. 75:43-51(1998).
RN [4]
RP INDUCTION.
RX PubMed=11091076; DOI=10.1016/s0925-4773(00)00480-9;
RA Nakata K., Koyabu Y., Aruga J., Mikoshiba K.;
RT "A novel member of the Xenopus Zic family, Zic5, mediates neural crest
RT development.";
RL Mech. Dev. 99:83-91(2000).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15843410; DOI=10.1242/dev.01823;
RA Sato T., Sasai N., Sasai Y.;
RT "Neural crest determination by co-activation of Pax3 and Zic1 genes in
RT Xenopus ectoderm.";
RL Development 132:2355-2363(2005).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16307736; DOI=10.1016/j.ydbio.2005.10.004;
RA Tropepe V., Li S., Dickinson A., Gamse J.T., Sive H.L.;
RT "Identification of a BMP inhibitor-responsive promoter module required for
RT expression of the early neural gene zic1.";
RL Dev. Biol. 289:517-529(2006).
RN [7]
RP FUNCTION.
RX PubMed=16871635; DOI=10.1002/dvdy.20896;
RA Li S., Shin Y., Cho K.W., Merzdorf C.S.;
RT "The Xfeb gene is directly upregulated by Zic1 during early neural
RT development.";
RL Dev. Dyn. 235:2817-2827(2006).
RN [8]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16871625; DOI=10.1002/dvdy.20906;
RA Fujimi T.J., Mikoshiba K., Aruga J.;
RT "Xenopus Zic4: conservation and diversification of expression profiles and
RT protein function among the Xenopus Zic family.";
RL Dev. Dyn. 235:3379-3386(2006).
RN [9]
RP FUNCTION.
RX PubMed=16892174; DOI=10.1387/ijdb.052110cm;
RA Merzdorf C.S., Sive H.L.;
RT "The zic1 gene is an activator of Wnt signaling.";
RL Int. J. Dev. Biol. 50:611-617(2006).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17409353; DOI=10.1091/mbc.e06-11-1047;
RA Hong C.-S., Saint-Jeannet J.-P.;
RT "The activity of Pax3 and Zic1 regulates three distinct cell fates at the
RT neural plate border.";
RL Mol. Biol. Cell 18:2192-2202(2007).
CC -!- FUNCTION: Transcriptional activator that induces expression of multiple
CC genes including pax3, en2, snai2/slug, feb and a subset of wnt genes.
CC Has multiple key roles in the regulation of neural induction and
CC neurogenesis: acts as a neural competence factor, sensitizing the
CC presumptive neuroectoderm to respond to subsequent neuralizing signals.
CC Promotes both preplacodal cell fates and neural crest cell fates, two
CC of the cell populations that arise from the neural plate border.
CC Cooperates with pax3 in concert with wnt signaling to determine neural
CC crest fate. Synergizes with the bmp-inhibitor noggin/nog and acts
CC through the wnt pathway to induce expression of en2. May bind to the
CC minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
CC {ECO:0000269|PubMed:15843410, ECO:0000269|PubMed:16871635,
CC ECO:0000269|PubMed:16892174, ECO:0000269|PubMed:17409353,
CC ECO:0000269|PubMed:9435279, ECO:0000269|PubMed:9655809,
CC ECO:0000269|PubMed:9739105}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9655809}. Cytoplasm
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: During early gastrula stages, widely expressed in
CC the dorsal ectoderm. At mid-gastrula, expressed throughout the
CC presumptive neural plate and at late gastrula, expression gradually
CC diminishes in the dorsal midline and increases in the anterior folds.
CC By early neurula stage, expression becomes restricted to the lateral
CC edges of the neural plate, corresponding to the presumptive dorsal
CC neural plate and neural crest, and in flanking ectoderm. In early
CC tailbud stages (stages 22-23), expressed in the dorsal forebrain,
CC midbrain and hindbrain. Subsequently expressed in the telencephalon and
CC at the diencephalon/mesencephalon boundary. In the spinal cord,
CC expression is restricted to the dorsal most region including the roof
CC plate. Also expressed in the somites but not in eye vesicles. At larval
CC stages, expressed mainly in the dorsal neural tube throughout its
CC anteroposterior axis. {ECO:0000269|PubMed:15843410,
CC ECO:0000269|PubMed:16307736, ECO:0000269|PubMed:16871625,
CC ECO:0000269|PubMed:17409353, ECO:0000269|PubMed:9435279,
CC ECO:0000269|PubMed:9655809, ECO:0000269|PubMed:9739105}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically from the blastula stage
CC (stage 8) and peaks in the gastrula with lower expression persisting
CC into tailbud stages. {ECO:0000269|PubMed:16871625,
CC ECO:0000269|PubMed:9435279, ECO:0000269|PubMed:9655809,
CC ECO:0000269|PubMed:9739105}.
CC -!- INDUCTION: By chrd/chordin, zic1, zic2, zic3 and zic5. Suppressed by
CC bmp signaling. {ECO:0000269|PubMed:11091076,
CC ECO:0000269|PubMed:16307736, ECO:0000269|PubMed:9435279}.
CC -!- DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for
CC transcription activation (By similarity). Removal of the C-terminal
CC regulatory domain increases activity. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AF022927; AAC14214.1; -; mRNA.
DR EMBL; AF028805; AAB99946.1; -; mRNA.
DR EMBL; AB009564; BAA33406.1; -; mRNA.
DR RefSeq; NP_001083799.1; NM_001090330.1.
DR AlphaFoldDB; O73689; -.
DR SMR; O73689; -.
DR GeneID; 399124; -.
DR KEGG; xla:399124; -.
DR CTD; 399124; -.
DR Xenbase; XB-GENE-864815; zic1.S.
DR OMA; HICVWEE; -.
DR OrthoDB; 768287at2759; -.
DR Proteomes; UP000186698; Chromosome 5S.
DR Bgee; 399124; Expressed in gastrula and 6 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0021904; P:dorsal/ventral neural tube patterning; IMP:UniProtKB.
DR GO; GO:0014033; P:neural crest cell differentiation; IMP:UniProtKB.
DR GO; GO:0014034; P:neural crest cell fate commitment; IMP:UniProtKB.
DR GO; GO:0014029; P:neural crest formation; IMP:UniProtKB.
DR GO; GO:0001840; P:neural plate development; IMP:UniProtKB.
DR GO; GO:0022008; P:neurogenesis; IMP:UniProtKB.
DR GO; GO:0042307; P:positive regulation of protein import into nucleus; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0030177; P:positive regulation of Wnt signaling pathway; IGI:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR041643; Znf_ZIC.
DR Pfam; PF00096; zf-C2H2; 3.
DR Pfam; PF18366; zf_ZIC; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; Developmental protein; Differentiation; DNA-binding;
KW Metal-binding; Neurogenesis; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Wnt signaling pathway; Zinc;
KW Zinc-finger.
FT CHAIN 1..443
FT /note="Zinc finger protein ZIC 1"
FT /id="PRO_0000047246"
FT ZN_FING 221..256
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 265..292
FT /note="C2H2-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 298..322
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 328..352
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 358..380
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 371..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 408..443
FT /note="Negatively regulates transcriptional activity"
FT COMPBIAS 382..433
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 5
FT /note="A -> T (in Ref. 2; AAB99946)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 48253 MW; E6E1CA481CF4C106 CRC64;
MLLDAGAQYP AIGVTTFGSS RHHSAGDVTD REVALGINPF ADGMGAFKLN PSSHDLASGQ
TAFTSQAPGY AAAALGHHHH PGHVSSYSSA AFNSTRDFLF RNRGFGEAAS AQHSLFASAA
GGFPGPHGPH ADTTGHLIFP GLHEQAASHA SPNVVNGQMR LGFSGDMYGR PDQYGQVTSP
RSEHYASSQL HGYGPMNMNM AAHHGAGAFF RYMRQPIKQE LICKWIEPEQ LANPKKSCNK
TFSTMHELVT HVTVEHVGGP EQSNHICVWE ECPREGKPFK AKYKLINHIR VHTGEKPFPC
PFPGCGKVFA RSENLKIHKR THTGEKPFKC EFEGCDRRFA NSSDRKKHMH VHTSDKPYLC
KMCDKSYTHP SSLRKHMKVH EASSQGSQPS PAASSGYESS TPPTIVSPSA ENQSTSSLSP
SSSAVHHTSN HSTLSSNFNE WYV
