ZIC2A_XENLA
ID ZIC2A_XENLA Reviewed; 503 AA.
AC Q91689; O93487; Q641A3;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Zinc finger protein ZIC 2-A;
DE AltName: Full=Zinc finger DNA-binding protein fZic;
DE AltName: Full=Zinc finger protein ZIC 2;
DE Short=XlZic2;
DE Short=xZic2;
DE AltName: Full=Zinc finger protein of the cerebellum 2-A;
GN Name=zic2-a; Synonyms=zic2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=9739105; DOI=10.1016/s0925-4773(98)00073-2;
RA Nakata K., Nagai T., Aruga J., Mikoshiba K.;
RT "Xenopus Zic family and its role in neural and neural crest development.";
RL Mech. Dev. 75:43-51(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Neurula;
RX PubMed=9634234; DOI=10.1038/31242;
RA Brewster R., Lee J., Ruiz i Altaba A.;
RT "Gli/Zic factors pattern the neural plate by defining domains of cell
RT differentiation.";
RL Nature 393:579-583(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Oocyte;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 226-302, FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=16207750; DOI=10.1242/dev.02066;
RA Houston D.W., Wylie C.;
RT "Maternal Xenopus Zic2 negatively regulates Nodal-related gene expression
RT during anteroposterior patterning.";
RL Development 132:4845-4855(2005).
RN [5]
RP INDUCTION.
RX PubMed=11091076; DOI=10.1016/s0925-4773(00)00480-9;
RA Nakata K., Koyabu Y., Aruga J., Mikoshiba K.;
RT "A novel member of the Xenopus Zic family, Zic5, mediates neural crest
RT development.";
RL Mech. Dev. 99:83-91(2000).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16871625; DOI=10.1002/dvdy.20906;
RA Fujimi T.J., Mikoshiba K., Aruga J.;
RT "Xenopus Zic4: conservation and diversification of expression profiles and
RT protein function among the Xenopus Zic family.";
RL Dev. Dyn. 235:3379-3386(2006).
CC -!- FUNCTION: Transcriptional repressor that inhibits neurogenesis and
CC induces neural and neural crest differentiation. Regulates
CC anteroposterior patterning in early development by inhibiting
CC expression of the nodal genes through the inhibition of vegt. Required
CC for gastrulation movements and for proper anterior neural and axial
CC development. May also act as a transcriptional activator. May bind to
CC the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
CC {ECO:0000269|PubMed:16207750, ECO:0000269|PubMed:9634234,
CC ECO:0000269|PubMed:9739105}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9634234}. Cytoplasm
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in both animal and vegetal regions of the
CC oocyte. At blastula and gastrula stages (stages 9 to 10.5), expressed
CC throughout the ectoderm. During late gastrula to neurula stages,
CC expression gradually diminishes in the midline region of the neural
CC plate and increases in the anterior neural folds, and continues to be
CC expressed in the posterior medial part of the neural plate. In early
CC tailbud stages (stages 22-23), expressed in the dorsal forebrain,
CC midbrain and hindbrain. Subsequently expressed in the telencephalon and
CC diencephalon/mesencephalon boundary. In the spinal cord, expression is
CC restricted to the dorsal most region including the roof plate. Also
CC expressed in the somites and eye vesicles. In the eye, expression is
CC restricted to the ciliary marginal zone of neural retina and is absent
CC from the lens. {ECO:0000269|PubMed:16207750,
CC ECO:0000269|PubMed:16871625, ECO:0000269|PubMed:9634234,
CC ECO:0000269|PubMed:9739105}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Continuously expressed from the egg to the tailbud stage (stage 30)
CC with an increase in expression at the early gastrula stage (stage 10).
CC {ECO:0000269|PubMed:16207750, ECO:0000269|PubMed:16871625,
CC ECO:0000269|PubMed:9739105}.
CC -!- INDUCTION: By zic1, zic2, zic3 and zic5. {ECO:0000269|PubMed:11091076}.
CC -!- DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for
CC transcription activation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AB009565; BAA33407.1; -; mRNA.
DR EMBL; U57453; AAC80229.1; -; mRNA.
DR EMBL; BC082436; AAH82436.1; -; mRNA.
DR RefSeq; NP_001081193.1; NM_001087724.1.
DR AlphaFoldDB; Q91689; -.
DR SMR; Q91689; -.
DR MaxQB; Q91689; -.
DR DNASU; 397704; -.
DR GeneID; 397704; -.
DR KEGG; xla:397704; -.
DR CTD; 397704; -.
DR Xenbase; XB-GENE-482658; zic2.L.
DR OrthoDB; 768287at2759; -.
DR Proteomes; UP000186698; Chromosome 2L.
DR Bgee; 397704; Expressed in blastula and 10 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:UniProtKB.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:UniProtKB.
DR GO; GO:0050768; P:negative regulation of neurogenesis; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0014034; P:neural crest cell fate commitment; IMP:UniProtKB.
DR GO; GO:0014029; P:neural crest formation; IMP:UniProtKB.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007601; P:visual perception; ISS:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR041643; Znf_ZIC.
DR Pfam; PF00096; zf-C2H2; 3.
DR Pfam; PF18366; zf_ZIC; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; Developmental protein; Differentiation; DNA-binding;
KW Gastrulation; Metal-binding; Neurogenesis; Nucleus; Reference proteome;
KW Repeat; Repressor; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..503
FT /note="Zinc finger protein ZIC 2-A"
FT /id="PRO_0000047249"
FT ZN_FING 277..312
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 321..348
FT /note="C2H2-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 354..378
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 384..408
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 414..436
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 61..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 20..21
FT /note="Missing (in Ref. 1; BAA33407)"
FT /evidence="ECO:0000305"
FT CONFLICT 32..33
FT /note="Missing (in Ref. 3; AAH82436)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="A -> G (in Ref. 1; BAA33407)"
FT /evidence="ECO:0000305"
FT CONFLICT 75..76
FT /note="Missing (in Ref. 2; AAC80229)"
FT /evidence="ECO:0000305"
FT CONFLICT 422
FT /note="S -> T (in Ref. 1; BAA33407)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="S -> T (in Ref. 1; BAA33407)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 503 AA; 53941 MW; 3F173F6648C8E806 CRC64;
MLLDAGPQFP ALGVGTFARH HHHHHHHHAA VAAAAAAAAE MQERELSLAQ NTFVEPTHMA
AFKLNPGGGS GGGSGGGGGG GGAGPNGGAG ASGPHDLSPP GQTSAFTSQA GYPTSALAPH
SAYSGAAAFN SPRDFLFRGR GFAEGSAAAG GGQHGLFGPP AGSLHHHPHH HHQLSHAEHP
QGHLLFPGIH DQHAAASQNT LGGQMRLGLP GEVFGRTEQY RQVSSPRGDP YTAAQLHNQY
SPMNMGMNMA AHHHHHHHHH PGAFFRYMRQ PCIKQELICK WIDPEQLNNP KKSCTKTFST
MHELVTHVSV EHVGGPEQSN HICFWEECPR EGKPFKAKYK LVNHIRVHTG EKPFPCPFPG
CGKVFARSEN LKIHKRTHTG EKPFQCEFEG CDRRFANSSD RKKHMHVHTS DKPYLCKMCD
KSYTHPSSLR KHMKVHESSP QGSESSPAAS SGYESSTPPG LVSPNSETQN PNLSPAAAAV
SAVHNVSSGA SGALASNFNE WYV
