ZIC3_XENLA
ID ZIC3_XENLA Reviewed; 441 AA.
AC O57311; Q640K7; Q6PF77; Q7ZZN6; Q7ZZN7; Q9DG65;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 3.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Zinc finger protein ZIC 3;
DE Short=XZic3;
DE Short=XlZic3;
DE AltName: Full=Zinc finger protein Zic3-A;
DE AltName: Full=Zinc finger protein of the cerebellum 3;
GN Name=zic3; Synonyms=zic3-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC TISSUE=Neurula;
RX PubMed=9342348; DOI=10.1073/pnas.94.22.11980;
RA Nakata K., Nagai T., Aruga J., Mikoshiba K.;
RT "Xenopus Zic3, a primary regulator both in neural and neural crest
RT development.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:11980-11985(1997).
RN [2]
RP SEQUENCE REVISION TO 17.
RA Nakata K., Nagai T., Aruga J., Mikoshiba K.;
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33, AND INDUCTION.
RX PubMed=15557819; DOI=10.1038/emm.2004.59;
RA Lee S.Y., Lee H.-S., Moon J.-S., Kim J.-I., Park J.-B., Lee J.-Y.,
RA Park M.J., Kim J.;
RT "Transcriptional regulation of Zic3 by heterodimeric AP-1(c-Jun/c-Fos)
RT during Xenopus development.";
RL Exp. Mol. Med. 36:468-475(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 319-331 AND 374-386.
RX PubMed=12963115; DOI=10.1016/s0925-4773(03)00082-0;
RA Weber J.R., Sokol S.Y.;
RT "Identification of a phylogenetically conserved activin-responsive enhancer
RT in the Zic3 gene.";
RL Mech. Dev. 120:955-964(2003).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=9739105; DOI=10.1016/s0925-4773(98)00073-2;
RA Nakata K., Nagai T., Aruga J., Mikoshiba K.;
RT "Xenopus Zic family and its role in neural and neural crest development.";
RL Mech. Dev. 75:43-51(1998).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, DOMAIN, AND INDUCTION.
RX PubMed=11044394; DOI=10.1242/dev.127.22.4787;
RA Kitaguchi T., Nagai T., Nakata K., Aruga J., Mikoshiba K.;
RT "Zic3 is involved in the left-right specification of the Xenopus embryo.";
RL Development 127:4787-4795(2000).
RN [8]
RP INDUCTION.
RX PubMed=11091076; DOI=10.1016/s0925-4773(00)00480-9;
RA Nakata K., Koyabu Y., Aruga J., Mikoshiba K.;
RT "A novel member of the Xenopus Zic family, Zic5, mediates neural crest
RT development.";
RL Mech. Dev. 99:83-91(2000).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=11869292; DOI=10.1046/j.1440-169x.2002.00624.x;
RA Kitaguchi T., Mizugishi K., Hatayama M., Aruga J., Mikoshiba K.;
RT "Xenopus Brachyury regulates mesodermal expression of Zic3, a gene
RT controlling left-right asymmetry.";
RL Dev. Growth Differ. 44:55-61(2002).
RN [10]
RP INDUCTION.
RX PubMed=14499648; DOI=10.1016/s0012-1606(03)00298-7;
RA Leclerc C., Lee M., Webb S.E., Moreau M., Miller A.L.;
RT "Calcium transients triggered by planar signals induce the expression of
RT ZIC3 gene during neural induction in Xenopus.";
RL Dev. Biol. 261:381-390(2003).
RN [11]
RP INDUCTION.
RX PubMed=16631621; DOI=10.1016/j.bbrc.2006.03.235;
RA Kelly L.E., Carrel T.L., Herman G.E., El-Hodiri H.M.;
RT "Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter
RT through a highly conserved region.";
RL Biochem. Biophys. Res. Commun. 344:1031-1037(2006).
RN [12]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16871625; DOI=10.1002/dvdy.20906;
RA Fujimi T.J., Mikoshiba K., Aruga J.;
RT "Xenopus Zic4: conservation and diversification of expression profiles and
RT protein function among the Xenopus Zic family.";
RL Dev. Dyn. 235:3379-3386(2006).
CC -!- FUNCTION: Probably acts as a transcriptional activator. May bind to the
CC minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Can determine the
CC ectodermal cell fate and promote the earliest step of neural and neural
CC crest development. Involved in establishing left-right asymmetry in the
CC embryo. {ECO:0000269|PubMed:11044394, ECO:0000269|PubMed:11869292,
CC ECO:0000269|PubMed:9342348}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: First detected at early gastrula (stage 10.25) in
CC the dorsal lip and prospective neural plate. Also expressed in the
CC mesoderm at early gastrulation, with expression strongest on the dorsal
CC side. Mesodermal expression continues at stage 12 but is hardly
CC detectable after stage 14. As gastrulation proceeds, expression
CC decreases in the dorsal lip and increases in the prospective neural
CC plate. At the neural plate stage (stage 14), expressed strongly in the
CC prospective mesencephalon and anterior rhombencephalon, after which
CC expression becomes stronger in the anterior neural folds. At early
CC tailbud stage (stage 20), expression becomes restricted to the dorsal
CC region of forebrain, midbrain and hindbrain, and weakly to the dorsal
CC trunk. After mid-tailbud stage, expression decreases in the
CC diencephalon, appears in the lateral mesoderm of the tailbud region and
CC becomes restricted in the dorsal part of the neural tube.
CC {ECO:0000269|PubMed:11044394, ECO:0000269|PubMed:16871625,
CC ECO:0000269|PubMed:9342348}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically. First expressed from the
CC late blastula. {ECO:0000269|PubMed:16871625,
CC ECO:0000269|PubMed:9342348, ECO:0000269|PubMed:9739105}.
CC -!- INDUCTION: By dvr1/vg1, activin and t/bra in the mesoderm. By
CC heteromeric AP-1 (fos/jun) during activin signaling. By increases in
CC Ca(2+) in the dorsal ectoderm, triggered by planar signals from the
CC mesoderm. Suppressed by bmp-signaling. By pbx1 and meis1. By zic1, zic2
CC and zic3 but not zic5. {ECO:0000269|PubMed:11044394,
CC ECO:0000269|PubMed:11091076, ECO:0000269|PubMed:11869292,
CC ECO:0000269|PubMed:14499648, ECO:0000269|PubMed:15557819,
CC ECO:0000269|PubMed:16631621, ECO:0000269|PubMed:9342348}.
CC -!- DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for
CC transcription activation (By similarity). The zinc fingers and the N-
CC terminus are independently important for establishing the L/R axis.
CC {ECO:0000250, ECO:0000269|PubMed:11044394}.
CC -!- SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH57699.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB005292; BAA23874.2; -; mRNA.
DR EMBL; BC057699; AAH57699.2; ALT_INIT; mRNA.
DR EMBL; BC082617; AAH82617.1; -; mRNA.
DR EMBL; AF179297; AAG09377.1; -; Genomic_DNA.
DR EMBL; AF506277; AAP20808.1; -; Genomic_DNA.
DR EMBL; AF506278; AAP20809.1; -; Genomic_DNA.
DR RefSeq; NP_001081088.1; NM_001087619.1.
DR AlphaFoldDB; O57311; -.
DR SMR; O57311; -.
DR PRIDE; O57311; -.
DR DNASU; 394375; -.
DR GeneID; 394375; -.
DR KEGG; xla:394375; -.
DR CTD; 394375; -.
DR Xenbase; XB-GENE-865376; zic3.S.
DR OMA; HESQCSE; -.
DR OrthoDB; 768287at2759; -.
DR Proteomes; UP000186698; Chromosome 8S.
DR Bgee; 394375; Expressed in gastrula and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:UniProtKB.
DR GO; GO:0000578; P:embryonic axis specification; IMP:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0014034; P:neural crest cell fate commitment; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR041643; Znf_ZIC.
DR Pfam; PF00096; zf-C2H2; 3.
DR Pfam; PF18366; zf_ZIC; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; Developmental protein; Differentiation; DNA-binding;
KW Metal-binding; Neurogenesis; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..441
FT /note="Zinc finger protein ZIC 3"
FT /id="PRO_0000047252"
FT ZN_FING 222..257
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 266..293
FT /note="C2H2-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 299..323
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 329..353
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 359..381
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 375..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 384..433
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 17
FT /note="G -> R (in Ref. 4; AAG09377)"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="S -> N (in Ref. 1; BAA23874)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="Q -> P (in Ref. 1; BAA23874)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 441 AA; 48485 MW; 5D7959E98217E886 CRC64;
MTMLLDGGPQ FPTLGVGGFG TARHHEMSNR DAGMGLNPFT EPSHAAAFKL SPASHDLSSS
QSSAFTPQAS GYASSLGHHA GQVPSYGGAA FNSTRDFLFR NRNSGIADSS SAGSQHGLFA
NHGPPGIGEP PGHLIFPGLH EQSSSHTSSN GHVVNGQMHL GLRGDIFGRP DPYRAVPSPR
TDHYAAAQFH NYNHMNMSMN VAAHHGQGAF FRYMRQPIKQ ELSCKWLEES TMNHPQKTCD
RTFSSMHELV THMTMEHVGG PEQNNHICYW EECPRGGKSF KAKYKLVNHI RVHTGEKPFP
CPFPGCGKIF ARSENLKIHK RTHTGEKPFK CEFEGCDRRF ANSSDRKKHM HVHTSDKPYI
CKVCDKSYTH PSSLRKHMKV HESQGSDSSP AASSGYESAT PPAMVSANSE EPSKNSSATH
QTNNNSHNTG LLPPNFNEWY V
