ZIF1_ARATH
ID ZIF1_ARATH Reviewed; 486 AA.
AC Q8RWN2; Q9FKI8;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Protein ZINC INDUCED FACILITATOR 1;
GN Name=ZIF1; OrderedLocusNames=At5g13740; ORFNames=MSH12.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INDUCTION BY NEMATODES.
RX PubMed=16478044; DOI=10.1094/mpmi-18-1247;
RA Hammes U.Z., Schachtman D.P., Berg R.H., Nielsen E., Koch W.,
RA McIntyre L.M., Taylor C.G.;
RT "Nematode-induced changes of transporter gene expression in Arabidopsis
RT roots.";
RL Mol. Plant Microbe Interact. 18:1247-1257(2005).
RN [6]
RP FUNCTION, MUTAGENESIS OF GLY-472, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP INDUCTION BY METAL, AND DISRUPTION PHENOTYPE.
RX PubMed=17277087; DOI=10.1104/pp.106.092015;
RA Haydon M.J., Cobbett C.S.;
RT "A novel major facilitator superfamily protein at the tonoplast influences
RT zinc tolerance and accumulation in Arabidopsis.";
RL Plant Physiol. 143:1705-1719(2007).
CC -!- FUNCTION: Major facilitator superfamily (MFS) transporter involved in
CC zinc tolerance by participating in vacuolar sequestration of zinc.
CC {ECO:0000269|PubMed:17277087}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:17277087};
CC Multi-pass membrane protein {ECO:0000269|PubMed:17277087}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in developing leaves,
CC differentiating zones of root tips and sepals of developing flowers.
CC Restricted to vascular tissues in older leaves, mature roots, flowers,
CC anthers and filaments. Not expressed in developing anthers.
CC {ECO:0000269|PubMed:17277087}.
CC -!- INDUCTION: Up-regulated by zinc and manganese. Not induced by cadmium,
CC copper or iron. Down-regulated upon nematode infection.
CC {ECO:0000269|PubMed:16478044, ECO:0000269|PubMed:17277087}.
CC -!- DISRUPTION PHENOTYPE: Zinc sensitivity resulting in increased chlorosis
CC and decreased shoot fresh weight and root length. Accumulation of high
CC levels of zinc in shoots. {ECO:0000269|PubMed:17277087}.
CC -!- MISCELLANEOUS: Overexpression of ZIF1 confers increased zinc tolerance
CC and causes interveinal leaf chlorosis.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM12978.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB10595.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB011484; BAB10595.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB006704; BAB10595.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED91934.1; -; Genomic_DNA.
DR EMBL; AY092979; AAM12978.1; ALT_FRAME; mRNA.
DR EMBL; AY128800; AAM91200.1; -; mRNA.
DR RefSeq; NP_196878.2; NM_121377.5.
DR AlphaFoldDB; Q8RWN2; -.
DR STRING; 3702.AT5G13740.1; -.
DR TCDB; 2.A.1.2.58; the major facilitator superfamily (mfs).
DR PaxDb; Q8RWN2; -.
DR PRIDE; Q8RWN2; -.
DR ProteomicsDB; 232306; -.
DR EnsemblPlants; AT5G13740.1; AT5G13740.1; AT5G13740.
DR GeneID; 831219; -.
DR Gramene; AT5G13740.1; AT5G13740.1; AT5G13740.
DR KEGG; ath:AT5G13740; -.
DR Araport; AT5G13740; -.
DR TAIR; locus:2173254; AT5G13740.
DR eggNOG; KOG2615; Eukaryota.
DR HOGENOM; CLU_001265_54_2_1; -.
DR InParanoid; Q8RWN2; -.
DR OMA; GDWVESP; -.
DR OrthoDB; 1090653at2759; -.
DR PhylomeDB; Q8RWN2; -.
DR PRO; PR:Q8RWN2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RWN2; baseline and differential.
DR Genevisible; Q8RWN2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022821; F:potassium ion antiporter activity; IBA:GO_Central.
DR GO; GO:0090333; P:regulation of stomatal closure; IBA:GO_Central.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0010043; P:response to zinc ion; IEP:TAIR.
DR GO; GO:0055069; P:zinc ion homeostasis; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..486
FT /note="Protein ZINC INDUCED FACILITATOR 1"
FT /id="PRO_0000400080"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 472
FT /note="G->R: In zif1-3; zinc sensitivity."
FT /evidence="ECO:0000269|PubMed:17277087"
SQ SEQUENCE 486 AA; 53179 MW; 9255E3F707231A3A CRC64;
MAEEYKEALL EKQNYHDGCP GCKVEQMKQL RRGYPYLELS FVWIIVLSTS LPISSLYPFL
YYMIEDFGVA KTEKDIGFYA GFVGCSFMLG RALTSVFWGI VADRYGRKPI ILLGTISIAI
FNALFGLSSN FWMAIGTRFL LGSFNCLLGT MKAYASEIFR DEYQATAMSA VSTAWGIGLI
IGPALGGFLA QPADKYPNVF SQESLFGRFR YALPCFTISA FALLVTVLCC FIPETLHNHK
LDSLSHDDSY DILEAASHES SPSTGKAGKN ERKASQSLLK NWPLMSSIIV YCVLCLHDTA
YSEIFALWAN SPRKYGGLSY STNEVGTVLA ISGLGLFSFQ VFVYPLAEKL LGPVLVTRYA
GALMIPIQMS YPFIAGLSGL SLSLMLNCAS ILINVLSVSA ITGLLILQNR AVDQSQRGAA
NGIAMTAMSL FKTVGPAGAG ILFSWSERRL NAAFLPGSHM VFFVLNVIVV VGVALTFKPF
LTTSRR
