ID   ZIF1_CAEEL              Reviewed;         489 AA.
AC   P34482;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2004, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Zinc finger-interacting factor 1;
DE            Short=Protein zif-1;
GN   Name=zif-1; Synonyms=tag-6; ORFNames=F59B2.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PIE-1; POS-1; MEX-1;
RP   MEX-5; MEX-6 AND ELC-1, AND MUTAGENESIS OF CYS-447.
RX   PubMed=12894212; DOI=10.1038/nature01887.;
RA   DeRenzo C., Reese K.J., Seydoux G.;
RT   "Exclusion of germ plasm proteins from somatic lineages by cullin-dependent
RT   degradation.";
RL   Nature 424:685-689(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Probable substrate recognition component of a CBC (Cul2-
CC       ElonginB-ElonginC) E3 ubiquitin-protein ligase complex which mediates
CC       the ubiquitination and subsequent proteasomal degradation of target
CC       proteins. Ensures germline precursor cell asymmetry by targeting
CC       germline proteins for destruction if expressed in non-germline cells.
CC       This process also requires elc-1, cul-2, rbx-1 and ubc-2.
CC       {ECO:0000269|PubMed:12894212}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Probable component of the CBC(zif-1) E3 ubiquitin-protein
CC       ligase complex formed of cul-2, elc-1, rbx-1 and zif-1. Interacts with
CC       pie-1, pos-1, mex-1, mex-5, mex-6 and elc-1.
CC       {ECO:0000269|PubMed:12894212}.
CC   -!- INTERACTION:
CC       P34482; Q94131: pie-1; NbExp=2; IntAct=EBI-300497, EBI-300501;
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DR   EMBL; AY547284; AAS49906.1; -; mRNA.
DR   EMBL; Z11505; CAA77583.2; -; Genomic_DNA.
DR   PIR; S31126; S31126.
DR   RefSeq; NP_498992.2; NM_066591.5.
DR   AlphaFoldDB; P34482; -.
DR   BioGRID; 41473; 6.
DR   IntAct; P34482; 6.
DR   STRING; 6239.F59B2.6; -.
DR   PaxDb; P34482; -.
DR   PRIDE; P34482; -.
DR   EnsemblMetazoa; F59B2.6.1; F59B2.6.1; WBGene00006977.
DR   GeneID; 176274; -.
DR   KEGG; cel:CELE_F59B2.6; -.
DR   UCSC; F59B2.6; c. elegans.
DR   CTD; 176274; -.
DR   WormBase; F59B2.6; CE34193; WBGene00006977; zif-1.
DR   eggNOG; ENOG502TH84; Eukaryota.
DR   HOGENOM; CLU_536634_0_0_1; -.
DR   InParanoid; P34482; -.
DR   OMA; IPRVCFL; -.
DR   OrthoDB; 881367at2759; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:P34482; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00006977; Expressed in adult organism and 4 other tissues.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
PE   1: Evidence at protein level;
KW   Developmental protein; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..489
FT                   /note="Zinc finger-interacting factor 1"
FT                   /id="PRO_0000066583"
FT   MUTAGEN         447
FT                   /note="C->F: Prevents interaction with elc-1."
FT                   /evidence="ECO:0000269|PubMed:12894212"
SQ   SEQUENCE   489 AA;  57228 MW;  306BBE2CDE6044DB CRC64;
     MSECSASTSQ LSTFCVNEIP WKLVPQTCDE FSEYEFIDEK YRRLSDVESF VWKWSPCGRY
     GIDLPGGRDV INQQLRDRMA VFDLHTNKWT LYTMDRGAFE IPRVCFLYWA RNDIIGTITL
     VKDYEAGPDD EIPLKFKQSF FYVSHETQTL VYSGSCKYRT NKTIVASYWY HIFENRRGEP
     FIDHNNYLWL VFSNGVDSLI LLPFIPTSTC FKAKCVSFDM NDIVSQFTGI RVFLDPFVSS
     HYVPWVYRND VNFFIRIDSH SFIEEQNRDY GLDPQIDNPE TKPHRGVFQL RVNLKDVIEK
     GEKDAIHAVN PETIETRFNR GDIELWRKIS DELRQEHQSI AQHGKMVVVQ RWSHFTVDMG
     LSKKMRTRVA DLDARDYCCS HKPRNSSFCC IDLDSERIKA VDALILGTDV VLPHPSGSVF
     LFRYKENYGM SYCELPFFQP LSLKLKCIQV LKESVEIDPS YYRIKCCLKM QEELEKETAE
     NLCTINIQQ