ID   ZIP2_ARATH              Reviewed;         353 AA.
AC   Q9LTH9; O81124;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Zinc transporter 2;
DE   AltName: Full=ZRT/IRT-like protein 2;
DE   Flags: Precursor;
GN   Name=ZIP2; OrderedLocusNames=At5g59520; ORFNames=F2O15.19;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=9618566; DOI=10.1073/pnas.95.12.7220;
RA   Grotz N., Fox T., Connolly E., Park W., Guerinot M.L., Eide D.;
RT   "Identification of a family of zinc transporter genes from Arabidopsis that
RT   respond to zinc deficiency.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:7220-7224(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   INDUCTION.
RX   PubMed=13129917; DOI=10.1074/jbc.m309338200;
RA   Wintz H., Fox T., Wu Y.-Y., Feng V., Chen W., Chang H.-S., Zhu T.,
RA   Vulpe C.D.;
RT   "Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal
RT   novel transporters involved in metal homeostasis.";
RL   J. Biol. Chem. 278:47644-47653(2003).
CC   -!- FUNCTION: Mediates zinc uptake. May also transport copper and cadmium
CC       ions. {ECO:0000269|PubMed:9618566}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: In roots by zinc and copper starvation.
CC       {ECO:0000269|PubMed:13129917}.
CC   -!- MISCELLANEOUS: Zinc uptake is highly pH-dependent and no uptake is seen
CC       at pH levels below 5.0. Inhibited by copper and cadmium ions.
CC   -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC       {ECO:0000305}.
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DR   EMBL; AF033536; AAC24198.1; -; mRNA.
DR   EMBL; AB025604; BAA97486.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97200.1; -; Genomic_DNA.
DR   PIR; T52184; T52184.
DR   RefSeq; NP_200760.1; NM_125344.3.
DR   AlphaFoldDB; Q9LTH9; -.
DR   SMR; Q9LTH9; -.
DR   STRING; 3702.AT5G59520.1; -.
DR   PaxDb; Q9LTH9; -.
DR   PRIDE; Q9LTH9; -.
DR   ProteomicsDB; 232305; -.
DR   EnsemblPlants; AT5G59520.1; AT5G59520.1; AT5G59520.
DR   GeneID; 836071; -.
DR   Gramene; AT5G59520.1; AT5G59520.1; AT5G59520.
DR   KEGG; ath:AT5G59520; -.
DR   Araport; AT5G59520; -.
DR   TAIR; locus:2148398; AT5G59520.
DR   eggNOG; KOG1558; Eukaryota.
DR   HOGENOM; CLU_046211_0_0_1; -.
DR   OMA; FGLLAKW; -.
DR   OrthoDB; 981397at2759; -.
DR   PhylomeDB; Q9LTH9; -.
DR   PRO; PR:Q9LTH9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LTH9; baseline and differential.
DR   Genevisible; Q9LTH9; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR   GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR003689; ZIP.
DR   Pfam; PF02535; Zip; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix; Transport; Zinc; Zinc transport.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..353
FT                   /note="Zinc transporter 2"
FT                   /id="PRO_0000041640"
FT   TOPO_DOM        30..59
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        81..90
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        112..130
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        152..201
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        223..235
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        257..263
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        285..296
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        318..331
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        353
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        13
FT                   /note="F -> V (in Ref. 1; AAC24198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        59
FT                   /note="S -> G (in Ref. 1; AAC24198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        80
FT                   /note="V -> I (in Ref. 1; AAC24198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        324
FT                   /note="R -> L (in Ref. 1; AAC24198)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   353 AA;  38313 MW;  D2CCB8FCD3552FC4 CRC64;
     MALSSKTLKS TLFFLSIIFL CFSLILAHGG IDDGDEEEET NQPPPATGTT TVVNLRSKSL
     VLVKIYCIII LFFSTFLAGV SPYFYRWNES FLLLGTQFSG GIFLATALIH FLSDANETFR
     GLKHKEYPYA FMLAAAGYCL TMLADVAVAF VAAGSNNNHV GASVGESRED DDVAVKEEGR
     REIKSGVDVS QALIRTSGFG DTALLIFALC FHSIFEGIAI GLSDTKSDAW RNLWTISLHK
     VFAAVAMGIA LLKLIPKRPF FLTVVYSFAF GISSPIGVGI GIGINATSQG AGGDWTYAIS
     MGLACGVFVY VAVNHLISKG YKPREECYFD KPIYKFIAVF LGVALLSVVM IWD