ZIP2_CAEEL
ID ZIP2_CAEEL Reviewed; 308 AA.
AC Q21148;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Transcription factor zip-2 {ECO:0000305};
GN Name=zip-2 {ECO:0000312|WormBase:K02F3.4};
GN ORFNames=K02F3.4 {ECO:0000312|WormBase:K02F3.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY P.AERUGINOSA INFECTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=20133860; DOI=10.1073/pnas.0914643107;
RA Estes K.A., Dunbar T.L., Powell J.R., Ausubel F.M., Troemel E.R.;
RT "bZIP transcription factor zip-2 mediates an early response to Pseudomonas
RT aeruginosa infection in Caenorhabditis elegans.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:2153-2158(2010).
RN [3] {ECO:0000305}
RP FUNCTION, AND INDUCTION BY P.AERUGINOSA INFECTION.
RX PubMed=22520464; DOI=10.1016/j.chom.2012.02.007;
RA McEwan D.L., Kirienko N.V., Ausubel F.M.;
RT "Host translational inhibition by Pseudomonas aeruginosa Exotoxin A
RT Triggers an immune response in Caenorhabditis elegans.";
RL Cell Host Microbe 11:364-374(2012).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY P.AERUGINOSA INFECTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=26876169; DOI=10.1016/j.celrep.2016.01.055;
RA Reddy K.C., Dunbar T.L., Nargund A.M., Haynes C.M., Troemel E.R.;
RT "The C. elegans CCAAT-Enhancer-Binding Protein Gamma Is Required for
RT Surveillance Immunity.";
RL Cell Rep. 14:1581-1589(2016).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28662060; DOI=10.1371/journal.pgen.1006876;
RA Tjahjono E., Kirienko N.V.;
RT "A conserved mitochondrial surveillance pathway is required for defense
RT against Pseudomonas aeruginosa.";
RL PLoS Genet. 13:e1006876-e1006876(2017).
RN [6] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32350153; DOI=10.18632/aging.103134;
RA Hahm J.H., Jeong C., Lee W., Koo H.J., Kim S., Hwang D., Nam H.G.;
RT "A cellular surveillance and defense system that delays aging phenotypes in
RT C. elegans.";
RL Aging (Albany NY) 12:8202-8220(2020).
CC -!- FUNCTION: Transcription factor that binds to the promoter and the
CC enhancer regions of target genes (By similarity). May act together with
CC the bZIP transcription factor, cebp-2 (PubMed:26876169). Involved in
CC responding to mitochondrial damage (PubMed:28662060, PubMed:32350153).
CC Plays a role in the delay of age-associated mitochondrial fragmentation
CC and muscle decline (PubMed:32350153). Has a protective role in response
CC to infection by the Gram-negative bacterium P.aeruginosa
CC (PubMed:26876169, PubMed:28662060, PubMed:20133860). Required to
CC prevent P.aeruginosa ToxA-mediated lethality (PubMed:22520464).
CC Required for the activation of infection response genes irg-1 and irg-2
CC following P.aeruginosa infection (PubMed:20133860).
CC {ECO:0000250|UniProtKB:P53567, ECO:0000269|PubMed:20133860,
CC ECO:0000269|PubMed:22520464, ECO:0000269|PubMed:26876169,
CC ECO:0000269|PubMed:28662060, ECO:0000269|PubMed:32350153}.
CC -!- INTERACTION:
CC Q21148; Q21361: atf-2; NbExp=2; IntAct=EBI-6731556, EBI-317743;
CC Q21148; Q8IG69: cebp-2; NbExp=3; IntAct=EBI-6731556, EBI-2914231;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26876169}.
CC -!- TISSUE SPECIFICITY: Expressed in the pharynx, as well as throughout the
CC intestine. {ECO:0000269|PubMed:20133860}.
CC -!- INDUCTION: Induced by P. aeruginosa infection.
CC {ECO:0000269|PubMed:20133860, ECO:0000269|PubMed:22520464,
CC ECO:0000269|PubMed:26876169}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown increases the proportion
CC of aged worms with fragmented mitochondria from 5% to 59% and causes a
CC 30% decrease in cellular ATP levels at day 8 in adult
CC (PubMed:32350153). RNAi-mediated knockdown decreases survival upon
CC infection with P.aeruginosa (PubMed:20133860, PubMed:28662060). RNAi-
CC mediated knockdown reduces induction of expression of infection
CC response gene, irg-1, in response to P.aeruginosa (PubMed:26876169,
CC PubMed:20133860). {ECO:0000269|PubMed:20133860,
CC ECO:0000269|PubMed:26876169, ECO:0000269|PubMed:28662060,
CC ECO:0000269|PubMed:32350153}.
CC -!- SIMILARITY: Belongs to the bZIP family. C/EBP subfamily. {ECO:0000305}.
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DR EMBL; BX284603; CCD61873.1; -; Genomic_DNA.
DR RefSeq; NP_497269.1; NM_064868.3.
DR AlphaFoldDB; Q21148; -.
DR SMR; Q21148; -.
DR IntAct; Q21148; 13.
DR STRING; 6239.K02F3.4.2; -.
DR PaxDb; Q21148; -.
DR EnsemblMetazoa; K02F3.4.1; K02F3.4.1; WBGene00019327.
DR EnsemblMetazoa; K02F3.4.2; K02F3.4.2; WBGene00019327.
DR EnsemblMetazoa; K02F3.4.3; K02F3.4.3; WBGene00019327.
DR GeneID; 175240; -.
DR UCSC; K02F3.4.2; c. elegans.
DR CTD; 175240; -.
DR WormBase; K02F3.4; CE23848; WBGene00019327; zip-2.
DR eggNOG; ENOG502SBKU; Eukaryota.
DR HOGENOM; CLU_892060_0_0_1; -.
DR InParanoid; Q21148; -.
DR OMA; DSLMSCD; -.
DR OrthoDB; 1563439at2759; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00019327; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0140367; P:antibacterial innate immune response; IMP:WormBase.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF07716; bZIP_2; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Immunity; Innate immunity; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..308
FT /note="Transcription factor zip-2"
FT /id="PRO_0000451176"
FT DOMAIN 242..305
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 217..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..276
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 277..291
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 217..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..277
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 308 AA; 34454 MW; 6C638E290F1AE336 CRC64;
MEPLSAPLAV GFSAWPPSPP LPPLAHSPLQ DAVHADHNHR RILNSCRNLP DCLTTTVPQL
HQINSAEQSP LQFTHQPNCD LIVVLDEQTT YTTTMTRRSL PKADRYLSEP NMSTPQASHG
SFEFDVPEYT SFDYAFDFPP VPEAFPPAAN SSSAASPDAD FLDLDSLMSC DITSLDAYIH
DDSIASPSNA SHISPPASDI DPVDEFFPQL VHNNKIQSSS SSTVETTITR GRKTSSVSSD
SSSDYRHKRD KNNLASQKSR QKRQAKIRES KEERERLEKR KVQLQAMVLT LETQVEDYKR
LVMMFVKR
