ZIP4_ARATH
ID ZIP4_ARATH Reviewed; 374 AA.
AC O04089; F4I7B9; Q56XS7;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Zinc transporter 4, chloroplastic;
DE AltName: Full=ZRT/IRT-like protein 4;
DE Flags: Precursor;
GN Name=ZIP4; OrderedLocusNames=At1g10970; ORFNames=T19D16.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=9618566; DOI=10.1073/pnas.95.12.7220;
RA Grotz N., Fox T., Connolly E., Park W., Guerinot M.L., Eide D.;
RT "Identification of a family of zinc transporter genes from Arabidopsis that
RT respond to zinc deficiency.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:7220-7224(1998).
RN [5]
RP INDUCTION.
RX PubMed=13129917; DOI=10.1074/jbc.m309338200;
RA Wintz H., Fox T., Wu Y.-Y., Feng V., Chen W., Chang H.-S., Zhu T.,
RA Vulpe C.D.;
RT "Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal
RT novel transporters involved in metal homeostasis.";
RL J. Biol. Chem. 278:47644-47653(2003).
CC -!- FUNCTION: May play a role in the transport of zinc in the plastids.
CC Could also transport copper ions. {ECO:0000269|PubMed:9618566}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- INDUCTION: In shoots and roots by zinc and copper starvation. Inhibited
CC by excess copper ions. {ECO:0000269|PubMed:13129917,
CC ECO:0000269|PubMed:9618566}.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC {ECO:0000305}.
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DR EMBL; U95973; AAB65480.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28670.2; -; Genomic_DNA.
DR EMBL; CP002684; ANM58220.1; -; Genomic_DNA.
DR EMBL; AK221596; BAD95131.1; -; mRNA.
DR PIR; F86243; F86243.
DR RefSeq; NP_001318977.1; NM_001331945.1.
DR RefSeq; NP_001320672.1; NM_001331946.1.
DR AlphaFoldDB; O04089; -.
DR STRING; 3702.AT1G10970.1; -.
DR PaxDb; O04089; -.
DR PRIDE; O04089; -.
DR ProteomicsDB; 242926; -.
DR EnsemblPlants; AT1G10970.1; AT1G10970.1; AT1G10970.
DR EnsemblPlants; AT1G10970.2; AT1G10970.2; AT1G10970.
DR GeneID; 837640; -.
DR Gramene; AT1G10970.1; AT1G10970.1; AT1G10970.
DR Gramene; AT1G10970.2; AT1G10970.2; AT1G10970.
DR KEGG; ath:AT1G10970; -.
DR Araport; AT1G10970; -.
DR eggNOG; KOG1558; Eukaryota.
DR HOGENOM; CLU_027089_3_0_1; -.
DR InParanoid; O04089; -.
DR OMA; WAGVSIM; -.
DR OrthoDB; 981397at2759; -.
DR PhylomeDB; O04089; -.
DR PRO; PR:O04089; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O04089; baseline and differential.
DR Genevisible; O04089; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR003689; ZIP.
DR InterPro; IPR004698; Zn/Fe_permease_fun/pln.
DR Pfam; PF02535; Zip; 1.
DR TIGRFAMs; TIGR00820; zip; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Ion transport; Membrane; Plastid; Reference proteome;
KW Thylakoid; Transit peptide; Transmembrane; Transmembrane helix; Transport;
KW Zinc; Zinc transport.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..374
FT /note="Zinc transporter 4, chloroplastic"
FT /id="PRO_0000041642"
FT TOPO_DOM ?..28
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..101
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..250
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..318
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 340..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 185..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 374 AA; 39286 MW; 82E570B58B4F1707 CRC64;
MASSTTKILC DAGESDLCRD DSAAFLLKFV AIASILLAGA AGVAIPLIGR NRRFLQTEGN
LFVAAKAFAA GVILATGFVH MLAGGTEALS NPCLPDFPWS KFPFPGFFAM VAALATLLVD
FMGTQYYERK QERNQAATEA AAGSEEIAVV PVVGERVTDN KVFGEEDGGG IHIVGIRAHA
AHHRHSHSNS HGTCDGHAHG HSHGHMHGNS DVENGARHVV VSQILELGIV SHSIIIGLSL
GVSQSPCTIR PLIAALSFHQ FFEGFALGGC ISQAQFRNKS ATIMACFFAL TTPLGIGIGT
AVASSFNSHS PGALVTEGIL DSLSAGILVY MALVDLIAAD FLSKRMSCNL RLQVVSYVML
FLGAGLMSAL AIWA
