ZIP8_ORYSJ
ID ZIP8_ORYSJ Reviewed; 390 AA.
AC A3BI11; Q7XJ42; Q7XJ47; Q8H385;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Zinc transporter 8;
DE AltName: Full=ZRT/IRT-like protein 8;
DE Short=OsZIP8;
DE Flags: Precursor;
GN Name=ZIP8; Synonyms=ZIP1; OrderedLocusNames=Os07g0232800, LOC_Os07g12890;
GN ORFNames=OsJ_23626, OSJNBa0061L20.103, OSJNBa0086N05.143;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Jiu Caiqing; TISSUE=Root;
RA Huang J., Zhang H.;
RT "Molecular characterization of a ZIP family in rice(Oryza sativa L.).";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Quanhong Y., Rihe P., Aisheng X.;
RT "An iron-regulated cation transporter from rice complements metal uptake-
RT deficient yeast mutants.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP INDUCTION.
RX PubMed=18038191; DOI=10.1007/s11033-007-9177-0;
RA Yang X., Huang J., Jiang Y., Zhang H.S.;
RT "Cloning and functional identification of two members of the ZIP (Zrt, Irt-
RT like protein) gene family in rice (Oryza sativa L.).";
RL Mol. Biol. Rep. 36:281-287(2009).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=20496122; DOI=10.1007/s10059-010-0069-0;
RA Lee S., Kim S.A., Lee J., Guerinot M.L., An G.;
RT "Zinc deficiency-inducible OsZIP8 encodes a plasma membrane-localized zinc
RT transporter in rice.";
RL Mol. Cells 29:551-558(2010).
CC -!- FUNCTION: Zinc transporter that may mediate zinc uptake from the
CC rhizosphere and may be responsible for the translocation of zinc within
CC the plant. {ECO:0000269|PubMed:20496122}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20496122};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20496122}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A3BI11-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A3BI11-2; Sequence=VSP_039756;
CC -!- INDUCTION: By zinc deficiency in roots and shoots.
CC {ECO:0000269|PubMed:18038191, ECO:0000269|PubMed:20496122}.
CC -!- MISCELLANEOUS: Plants overexpressing ZIP8 show decreased zinc
CC concentration in shoots and mature seeds, increased concentration in
CC roots, and are shorter than wild-type plants when grown in field.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC {ECO:0000305}.
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DR EMBL; AY324148; AAP88588.1; -; mRNA.
DR EMBL; AY327038; AAP92123.1; -; mRNA.
DR EMBL; AP005246; BAC21508.1; -; Genomic_DNA.
DR EMBL; AP005774; BAD31696.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21153.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00723.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ39200.1; -; Genomic_DNA.
DR RefSeq; XP_015644611.1; XM_015789125.1. [A3BI11-1]
DR AlphaFoldDB; A3BI11; -.
DR STRING; 39947.A3BI11; -.
DR TCDB; 2.A.5.1.5; the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.
DR PaxDb; A3BI11; -.
DR PRIDE; A3BI11; -.
DR EnsemblPlants; Os07t0232800-00; Os07t0232800-00; Os07g0232800. [A3BI11-2]
DR GeneID; 4342782; -.
DR Gramene; Os07t0232800-00; Os07t0232800-00; Os07g0232800. [A3BI11-2]
DR KEGG; osa:4342782; -.
DR eggNOG; KOG1558; Eukaryota.
DR InParanoid; A3BI11; -.
DR OMA; HHHGHFN; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IMP:UniProtKB.
DR InterPro; IPR003689; ZIP.
DR InterPro; IPR004698; Zn/Fe_permease_fun/pln.
DR Pfam; PF02535; Zip; 1.
DR TIGRFAMs; TIGR00820; zip; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport;
KW Zinc; Zinc transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..390
FT /note="Zinc transporter 8"
FT /id="PRO_0000398332"
FT TOPO_DOM 26..50
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..267
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..296
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..329
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 165..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..199
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 323
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 165..197
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_039756"
FT CONFLICT 7
FT /note="A -> D (in Ref. 1; AAP88588)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="K -> Q (in Ref. 2; AAP92123)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 390 AA; 40256 MW; 880AFCDEE37E8CFC CRC64;
MRTNTTATVL LAAAVALLLA TAARGDGGDG GCGKEDAAAG RDRARARGLK IAAFFSILVC
GALGCGLPSL GRHVPALRPD GDVFFLVKAF AAGVILATGF IHILPDAFDN LTDDCLPAGG
PWKEFPFAGF GAMVGAIGTL VVDTLATGYF TRALSKKDAA TAAAVADEEK QSAAATQQHN
HHHNHHVVGD GGGGGEEHEG QVHVHTHATH GHAHGSSALV AAVGEDDKET TLRHRVISQV
LELGIVVHSV IIGISLGASQ NPETIKPLVV ALSFHQMFEG MGLGGCIVQA KFKVRSIVTM
VLFFCLTTPV GIAVGVGISS VYNESSPTAL VVEGILNSVA AGILIYMALV DLLAEDFMNP
RVQSKGKLQL GINLAMLAGA GLMSMLAKWA
