ID   ZITB_ECOLI              Reviewed;         313 AA.
AC   P75757;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=Zinc transporter ZitB;
GN   Name=zitB; Synonyms=ybgR; OrderedLocusNames=b0752, JW0735;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   CHARACTERIZATION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=11443104; DOI=10.1128/jb.183.15.4664-4667.2001;
RA   Grass G., Fan B., Rosen B.P., Franke S., Nies D.H., Rensing C.;
RT   "ZitB (YbgR), a member of the cation diffusion facilitator family, is an
RT   additional zinc transporter in Escherichia coli.";
RL   J. Bacteriol. 183:4664-4667(2001).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Involved in zinc efflux across the cytoplasmic membrane, thus
CC       reducing zinc accumulation in the cytoplasm and rendering bacteria more
CC       resistant to zinc. It may contribute to zinc homeostasis at low
CC       concentrations of zinc, whereas ZntA is required for growth at more
CC       toxic concentrations.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: By zinc.
CC   -!- MISCELLANEOUS: Appears to be selective for zinc, not conferring
CC       resistance to cobalt nor cadmium.
CC   -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC       transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR   EMBL; U00096; AAC73839.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35414.1; -; Genomic_DNA.
DR   PIR; H64810; H64810.
DR   RefSeq; NP_415273.1; NC_000913.3.
DR   RefSeq; WP_000951292.1; NZ_SSZK01000002.1.
DR   AlphaFoldDB; P75757; -.
DR   SMR; P75757; -.
DR   BioGRID; 4261706; 29.
DR   STRING; 511145.b0752; -.
DR   TCDB; 2.A.4.1.4; the cation diffusion facilitator (cdf) family.
DR   PaxDb; P75757; -.
DR   PRIDE; P75757; -.
DR   EnsemblBacteria; AAC73839; AAC73839; b0752.
DR   EnsemblBacteria; BAA35414; BAA35414; BAA35414.
DR   GeneID; 66670977; -.
DR   GeneID; 945348; -.
DR   KEGG; ecj:JW0735; -.
DR   KEGG; eco:b0752; -.
DR   PATRIC; fig|1411691.4.peg.1527; -.
DR   EchoBASE; EB3426; -.
DR   eggNOG; COG1230; Bacteria.
DR   HOGENOM; CLU_013430_0_0_6; -.
DR   InParanoid; P75757; -.
DR   OMA; GHEKMLH; -.
DR   PhylomeDB; P75757; -.
DR   BioCyc; EcoCyc:B0752-MON; -.
DR   BioCyc; MetaCyc:B0752-MON; -.
DR   PRO; PR:P75757; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0005385; F:zinc ion transmembrane transporter activity; IMP:EcoCyc.
DR   GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0006829; P:zinc ion transport; IMP:EcoCyc.
DR   Gene3D; 1.20.1510.10; -; 1.
DR   Gene3D; 3.30.70.1350; -; 1.
DR   HAMAP; MF_00552; ZitB; 1.
DR   InterPro; IPR002524; Cation_efflux.
DR   InterPro; IPR036837; Cation_efflux_CTD_sf.
DR   InterPro; IPR027469; Cation_efflux_TMD_sf.
DR   InterPro; IPR023500; Zn_transptr_ZitB.
DR   Pfam; PF01545; Cation_efflux; 1.
DR   SUPFAM; SSF160240; SSF160240; 1.
DR   SUPFAM; SSF161111; SSF161111; 1.
DR   TIGRFAMs; TIGR01297; CDF; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW   Zinc transport.
FT   CHAIN           1..313
FT                   /note="Zinc transporter ZitB"
FT                   /id="PRO_0000206107"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        42..47
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        69..89
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..121
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        143..159
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        181
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..313
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   313 AA;  34678 MW;  36740D67EABAC907 CRC64;
     MAHSHSHTSS HLPEDNNARR LLYAFGVTAG FMLVEVVGGF LSGSLALLAD AGHMLTDTAA
     LLFALLAVQF SRRPPTIRHT FGWLRLTTLA AFVNAIALVV ITILIVWEAI ERFRTPRPVE
     GGMMMAIAVA GLLANILSFW LLHHGSEEKN LNVRAAALHV LGDLLGSVGA IIAALIIIWT
     GWTPADPILS ILVSLLVLRS AWRLLKDSVN ELLEGAPVSL DIAELKRRMC REIPEVRNVH
     HVHVWMVGEK PVMTLHVQVI PPHDHDALLD QIQHYLMDHY QIEHATIQME YQPCHGPDCH
     LNEGVSGHSH HHH