ID   ZN143_XENLA             Reviewed;         565 AA.
AC   Q91853; Q6IR49;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Zinc finger protein 143;
DE   AltName: Full=Selenocysteine tRNA gene transcription-activating factor;
GN   Name=znf143; Synonyms=staf;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND DNA-BINDING.
RC   TISSUE=Ovary;
RX   PubMed=7641696; DOI=10.1002/j.1460-2075.1995.tb00047.x;
RA   Schuster C., Myslinski E., Krol A., Carbon P.;
RT   "Staf, a novel zinc finger protein that activates the RNA polymerase III
RT   promoter of the selenocysteine tRNA gene.";
RL   EMBO J. 14:3777-3787(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcriptional activator. Activates the gene for
CC       selenocysteine tRNA (tRNAsec). Binds to the activator element (AE)
CC       motif of the selenocysteine tRNA gene promoter.
CC       {ECO:0000269|PubMed:7641696}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q91853-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q91853-2; Sequence=VSP_036981;
CC   -!- SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH71051.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA59354.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X84996; CAA59354.1; ALT_INIT; mRNA.
DR   EMBL; BC071051; AAH71051.1; ALT_INIT; mRNA.
DR   PIR; S58681; S58681.
DR   RefSeq; NP_001084373.1; NM_001090904.1.
DR   RefSeq; XP_018115027.1; XM_018259538.1. [Q91853-2]
DR   AlphaFoldDB; Q91853; -.
DR   SMR; Q91853; -.
DR   DNASU; 399467; -.
DR   GeneID; 399467; -.
DR   KEGG; xla:399467; -.
DR   CTD; 399467; -.
DR   Xenbase; XB-GENE-1012509; znf143.S.
DR   OrthoDB; 1318335at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 399467; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 5.
DR   SMART; SM00355; ZnF_C2H2; 7.
DR   SUPFAM; SSF57667; SSF57667; 3.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE   1: Evidence at protein level;
KW   Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..565
FT                   /note="Zinc finger protein 143"
FT                   /id="PRO_0000370719"
FT   ZN_FING         230..254
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         260..284
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         290..314
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         320..344
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         350..374
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         380..404
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         410..433
FT                   /note="C2H2-type 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   VAR_SEQ         493..538
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_036981"
SQ   SEQUENCE   565 AA;  61418 MW;  F83B5A070E2A0387 CRC64;
     MLLAQINRDS QAMAEFPGGG GEAQHVTLCL TEAVADGESM DTMEGMSLQA VTLADGSTAY
     IQHNTKDGKL IEGQVIQLED GSAAYVQHVP KGDDLSLEDG QAVQLEDGTT AYIHHSSKDS
     YDQSSVQAVQ LEDGTTAYIH HAVQVPQSDT ILAIQADGTV AGLHTGEASI DPDTISALEQ
     YAAKVSIEGG EGAGSNALIN ESESEKKMQI VLSHGARLPI KAQQTNEKAF RCDYEGCGKL
     YTTAHHLKVH ERSHTGDRPY QCDHGSCRKA FATGYGLKSH VRTHTGEKPY RCSEENCTKS
     FKTSGDLQKH VRTHTGERPF KCPFEGCGRS FTTSNIRKVH IRTHTGERPY YCSEPGCGRA
     FASATNYKNH VRIHTGEKPY VCTVPGCDKR FTEYSSLYKH HVVHTHSKPY NCNHCGKTYK
     QISTLAMHKR TAHNDTEPIE EDQESFFVPQ PPDEVIKGSQ ITYVTGVDGE DGISATQSGQ
     QLALIAQDGT SHVAIVAQDL SAFHNSETGP QHSHNLGGSD SRPVTLLATS NGRQIAVQIG
     EQQSLEEAIR IASRIQQGES PGMED