ZN197_HUMAN
ID ZN197_HUMAN Reviewed; 1029 AA.
AC O14709; B2RAH8; Q86VG0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 196.
DE RecName: Full=Zinc finger protein 197;
DE AltName: Full=Zinc finger protein with KRAB and SCAN domains 9;
DE AltName: Full=ZnF20;
DE AltName: Full=pVHL-associated KRAB domain-containing protein;
GN Name=ZNF197; Synonyms=ZKSCAN9, ZNF166;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9380504; DOI=10.1093/nar/25.19.3823;
RA Gonsky R., Knauf J.A., Elisei R., Wang J.W., Su S., Fagin J.A.;
RT "Identification of rapid turnover transcripts overexpressed in thyroid
RT tumors and thyroid cancer cell lines: use of a targeted differential RNA
RT display method to select for mRNA subsets.";
RL Nucleic Acids Res. 25:3823-3831(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND ALTERNATIVE SPLICING.
RX PubMed=12682018; DOI=10.1093/emboj/cdg173;
RA Li Z., Wang D., Na X., Schoen S.R., Messing E.M., Wu G.;
RT "The VHL protein recruits a novel KRAB-A domain protein to repress HIF-
RT 1alpha transcriptional activity.";
RL EMBO J. 22:1857-1867(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O14709-1; Sequence=Displayed;
CC Name=2; Synonyms=VHLaK;
CC IsoId=O14709-2; Sequence=VSP_043021, VSP_043022;
CC -!- MISCELLANEOUS: [Isoform 2]: Negative regulator of HIF1A
CC transactivation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF011573; AAB84385.1; -; mRNA.
DR EMBL; AY074878; AAL79677.1; -; mRNA.
DR EMBL; AK314196; BAG36875.1; -; mRNA.
DR EMBL; AC099669; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471055; EAW64714.1; -; Genomic_DNA.
DR CCDS; CCDS2717.1; -. [O14709-1]
DR CCDS; CCDS33743.1; -. [O14709-2]
DR RefSeq; NP_001020026.1; NM_001024855.2. [O14709-2]
DR RefSeq; NP_001310222.1; NM_001323293.1. [O14709-1]
DR RefSeq; NP_001310223.1; NM_001323294.1. [O14709-2]
DR RefSeq; NP_008922.1; NM_006991.4. [O14709-1]
DR AlphaFoldDB; O14709; -.
DR SMR; O14709; -.
DR BioGRID; 115470; 16.
DR IntAct; O14709; 17.
DR STRING; 9606.ENSP00000379370; -.
DR GlyGen; O14709; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; O14709; -.
DR PhosphoSitePlus; O14709; -.
DR BioMuta; ZNF197; -.
DR EPD; O14709; -.
DR jPOST; O14709; -.
DR MassIVE; O14709; -.
DR MaxQB; O14709; -.
DR PaxDb; O14709; -.
DR PeptideAtlas; O14709; -.
DR PRIDE; O14709; -.
DR ProteomicsDB; 48170; -. [O14709-1]
DR ProteomicsDB; 48171; -. [O14709-2]
DR ABCD; O14709; 3 sequenced antibodies.
DR Antibodypedia; 12503; 137 antibodies from 20 providers.
DR DNASU; 10168; -.
DR Ensembl; ENST00000344387.9; ENSP00000345809.4; ENSG00000186448.15. [O14709-1]
DR Ensembl; ENST00000383744.8; ENSP00000373250.4; ENSG00000186448.15. [O14709-2]
DR Ensembl; ENST00000383745.6; ENSP00000373251.2; ENSG00000186448.15. [O14709-2]
DR Ensembl; ENST00000396058.1; ENSP00000379370.1; ENSG00000186448.15. [O14709-1]
DR Ensembl; ENST00000627517.2; ENSP00000487305.1; ENSG00000281709.3. [O14709-2]
DR Ensembl; ENST00000627752.2; ENSP00000486777.1; ENSG00000281709.3. [O14709-2]
DR Ensembl; ENST00000627845.1; ENSP00000486739.1; ENSG00000281709.3. [O14709-1]
DR Ensembl; ENST00000628172.3; ENSP00000485709.1; ENSG00000281709.3. [O14709-1]
DR GeneID; 10168; -.
DR KEGG; hsa:10168; -.
DR MANE-Select; ENST00000344387.9; ENSP00000345809.4; NM_006991.5; NP_008922.1.
DR UCSC; uc003cnm.4; human. [O14709-1]
DR CTD; 10168; -.
DR DisGeNET; 10168; -.
DR GeneCards; ZNF197; -.
DR HGNC; HGNC:12988; ZNF197.
DR HPA; ENSG00000186448; Low tissue specificity.
DR MIM; 618359; gene.
DR neXtProt; NX_O14709; -.
DR OpenTargets; ENSG00000186448; -.
DR PharmGKB; PA37568; -.
DR VEuPathDB; HostDB:ENSG00000186448; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162801; -.
DR HOGENOM; CLU_002678_53_2_1; -.
DR InParanoid; O14709; -.
DR OMA; TVEEFSW; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; O14709; -.
DR TreeFam; TF350837; -.
DR PathwayCommons; O14709; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; O14709; -.
DR BioGRID-ORCS; 10168; 10 hits in 1102 CRISPR screens.
DR GenomeRNAi; 10168; -.
DR Pharos; O14709; Tdark.
DR PRO; PR:O14709; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; O14709; protein.
DR Bgee; ENSG00000186448; Expressed in cortical plate and 106 other tissues.
DR ExpressionAtlas; O14709; baseline and differential.
DR Genevisible; O14709; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 20.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 22.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 12.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 22.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 22.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..1029
FT /note="Zinc finger protein 197"
FT /id="PRO_0000047448"
FT DOMAIN 42..124
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT DOMAIN 217..289
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 370..392
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 398..420
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 426..448
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 454..476
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 482..504
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 510..532
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 538..560
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 566..588
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 594..616
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 622..644
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 650..672
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 678..700
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 706..728
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 734..756
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 762..784
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 790..812
FT /note="C2H2-type 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 818..840
FT /note="C2H2-type 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 846..868
FT /note="C2H2-type 18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 874..896
FT /note="C2H2-type 19"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 902..924
FT /note="C2H2-type 20"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 930..952
FT /note="C2H2-type 21"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 958..980
FT /note="C2H2-type 22"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 262..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 257..267
FT /note="EWETMTENEEV -> GYRKYRRQRNK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12682018"
FT /id="VSP_043021"
FT VAR_SEQ 268..1029
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12682018"
FT /id="VSP_043022"
SQ SEQUENCE 1029 AA; 118847 MW; 95DB6F65A170F76B CRC64;
MTRENVAHNA LRQEGLVKGK DDTWKWGTSF QGSSSSVWET SHLHFRQLRY HETSGPQEAL
SRLRELCRRW LRPEARTKAQ ILELLVLEQF LSILPGEIRT WVQLHHPGSG EEAVALVEEL
QKDLDGPAIQ VPVLVKDQDT LQKVVSAPGT TLPPVLPGSH IAAEICPHPP TDLVAFNLQD
PQHDSPAPEA SALSQEENPR NQLMALMLLT AQPQELVMFE EVSVCFTSEE WACLGPIQRA
LYWDVMLENY GNVTSLEWET MTENEEVTSK PSSSQRADSH KGTSKRLQGS VPQVLDFEEE
CEWQVLASQW GNETDERADT VKKVSLCERD KKKRTPPEKQ GQKWKELGDS LTFGSAISES
LIGTEGKKFY KCDMCCKHFN KISHLINHRR IHTGEKPHKC KECGKGFIQR SSLLMHLRNH
SGEKPYKCNE CGKAFSQSAY LLNHQRIHTG EKPYKCKECG KGFYRHSGLI IHLRRHSGER
PYKCNECGKV FSQNAYLIDH QRLHKGEEPY KCNKCQKAFI LKKSLILHQR IHSGEKPYKC
DECGKTFAQT TYLIDHQRLH SAENPYKCKE CGKVFIRSKS LLLHQRVHTE KKTFGCKKCG
KIFSSKSNFI DHKRMHSREK PYKCTECGKA FTQSAYLFDH QRLHNGEKPY ECNECGKVFI
LKKSLILHQR FHTGENLYEC KDCGKVFGSN RNLIDHERLH NGEKPYECRE CGKTFIMSKS
FMVHQKLHTQ EKAYKCEDCG KAFSYNSSLL VHRRIHTGEK PFECSECGRA FSSNRNLIEH
KRIHSGEKPY ECDECGKCFI LKKSLIGHQR IHTREKSYKC NDCGKVFSYR SNLIAHQRIH
TGEKPYACSE CGKGFTYNRN LIEHQRIHSG EKTYECHVCR KVLTSSRNLM VHQRIHTGEK
PYKCNECGKD FSQNKNLVVH QRMHTGEKPY ECDKCRKSFT SKRNLVGHQR IHTGEKPYGC
NDCSKVFRQR KNLTVHQKIH TDEKPCECDV SEKEFSQTSN LHLQQKIHTI EEFSWLQNTN
ESKIEIQKI
