ZN207_PONAB
ID ZN207_PONAB Reviewed; 494 AA.
AC Q5R8K4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=BUB3-interacting and GLEBS motif-containing protein ZNF207 {ECO:0000250|UniProtKB:O43670};
DE Short=BuGZ {ECO:0000250|UniProtKB:O43670};
DE AltName: Full=Zinc finger protein 207 {ECO:0000250|UniProtKB:O43670};
GN Name=ZNF207 {ECO:0000250|UniProtKB:O43670};
GN Synonyms=BUGZ {ECO:0000250|UniProtKB:O43670};
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Kinetochore- and microtubule-binding protein that plays a key
CC role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered
CC low-complexity regions and undergoes phase transition or coacervation
CC to form temperature-dependent liquid droplets. Coacervation promotes
CC microtubule bundling and concentrates tubulin, promoting microtubule
CC polymerization and assembly of spindle and spindle matrix by
CC concentrating its building blocks. Also acts as a regulator of mitotic
CC chromosome alignment by mediating the stability and kinetochore loading
CC of BUB3. Mechanisms by which BUB3 is protected are unclear: according
CC to a first report, ZNF207/BuGZ may act by blocking ubiquitination and
CC proteasomal degradation of BUB3. According to another report, the
CC stabilization is independent of the proteasome.
CC {ECO:0000250|UniProtKB:O43670}.
CC -!- SUBUNIT: Interacts (via GLEBS region) with BUB3.
CC {ECO:0000250|UniProtKB:O43670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43670}.
CC Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:O43670}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:O43670}.
CC Note=Localizes primarily to the nucleus in interphase, concentrates at
CC kinetochores prior to nuclear envelope breakdown and during early
CC prometaphase, and disappears from kinetochores upon microtubule-
CC binding. {ECO:0000250|UniProtKB:O43670}.
CC -!- DOMAIN: The GLEBS region mediates interaction with BUB3.
CC {ECO:0000250|UniProtKB:O43670}.
CC -!- DOMAIN: The microtubule-binding region is required for efficient
CC loading of BUB3 onto kinetochores and proper mitosis.
CC {ECO:0000250|UniProtKB:O43670}.
CC -!- DOMAIN: Mainly composed of disordered low-complexity regions outside of
CC the C2H2-type zinc fingers. Coacervation depends on hydrophobic and
CC aromatic Phe and Tyr in the disordered low-complexity region, that may
CC promote coacervation by forming intermolecular hydrophobic
CC interactions. {ECO:0000250|UniProtKB:Q9JMD0}.
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DR EMBL; CR859748; CAH91906.1; -; mRNA.
DR RefSeq; NP_001126104.1; NM_001132632.1.
DR AlphaFoldDB; Q5R8K4; -.
DR SMR; Q5R8K4; -.
DR STRING; 9601.ENSPPYP00000009173; -.
DR GeneID; 100173059; -.
DR CTD; 7756; -.
DR eggNOG; KOG2893; Eukaryota.
DR InParanoid; Q5R8K4; -.
DR Proteomes; UP000001595; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:1990047; C:spindle matrix; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0001578; P:microtubule bundle formation; ISS:UniProtKB.
DR GO; GO:0046785; P:microtubule polymerization; ISS:UniProtKB.
DR GO; GO:0090307; P:mitotic spindle assembly; ISS:UniProtKB.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Cytoplasm; Cytoskeleton; DNA-binding; Kinetochore; Metal-binding;
KW Microtubule; Mitosis; Nucleus; Reference proteome; Repeat; Zinc;
KW Zinc-finger.
FT CHAIN 1..494
FT /note="BUB3-interacting and GLEBS motif-containing protein
FT ZNF207"
FT /id="PRO_0000301676"
FT ZN_FING 11..34
FT /note="C2H2-type 1"
FT ZN_FING 35..58
FT /note="C2H2-type 2"
FT REGION 1..92
FT /note="Microtubule-binding region"
FT /evidence="ECO:0000250|UniProtKB:O43670"
FT REGION 100..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 375..407
FT /note="GLEBS"
FT /evidence="ECO:0000250|UniProtKB:O43670"
FT REGION 455..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..161
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..269
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 319..377
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..484
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 494 AA; 52580 MW; 28EE04241771AE46 CRC64;
MGRKKKKQLK PWCWYCNRDF DDEKILTQHQ KAKHFKCHIC HKKLYTGPGL AIHCMQVHKE
TIDAVPNAIP GRTDIELEIY GMEGIPEKDM DEGRRLLEQK TQESQKKKQQ DDSDEYDDDD
SAASTSFQPQ PVQPQQGYIP PMAQPGLPPV PGAPGMPPGI PPLMPGVPPL MPGMPPVMPG
MPPGLHHQRK YTQSFCGENI MMPMGGMMPP GPGIPPLMPG MPPGMPPPVP RPGIPPMTQA
QAVSAPGILN RPPAPTATVP APQPPVTKPL FPSAGQMGTP VTSSSTASSN SESLSASSKA
PFPSTAQAQV AVQGPVGTDF KPLNSTPATS TEPPKPTFPA YTQSTASTTS TTNSTAAKPA
ASITSKPATL TTTSATSKLI HPDEDISLEE RRAQLPKYQR NLPRPGQAPI GNPPVGPIGG
MMPPQPGIPQ QQGMRPPMPP HGQYGGHHQG MPGYLPGAMP PYGQGPPMVP PYQGGPPRPP
MGMRPPVMSQ GGRY
