ZN221_HUMAN
ID ZN221_HUMAN Reviewed; 617 AA.
AC Q9UK13; A0A087WT08; B2RAI6; K7EIT6; Q2M2H2; Q9P1U8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 4.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Zinc finger protein 221;
GN Name=ZNF221;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ILE-179; ARG-256; THR-519 AND
RP ARG-557.
RX PubMed=12743021; DOI=10.1101/gr.963903;
RA Shannon M., Hamilton A.T., Gordon L., Branscomb E., Stubbs L.;
RT "Differential expansion of zinc-finger transcription factor loci in
RT homologous human and mouse gene clusters.";
RL Genome Res. 13:1097-1110(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kodoyianni V., Ge Y., Krummel G.K., Kvikstad E., Grable L., Severin J.,
RA Gordon L., Shannon M., Brower A., Olsen A.S., Smith L.M.;
RT "Sequence analysis of a 1Mb region in 19q13.2 containing a zinc finger gene
RT cluster.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ILE-179; ARG-256;
RP THR-519 AND ARG-557.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANTS ILE-179;
RP ARG-256; THR-519 AND ARG-557.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ILE-179; ARG-256;
RP THR-519 AND ARG-557.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q9UK13; P60371: KRTAP10-6; NbExp=3; IntAct=EBI-14513896, EBI-12012928;
CC Q9UK13; P43361: MAGEA8; NbExp=3; IntAct=EBI-14513896, EBI-10182930;
CC Q9UK13; Q99750: MDFI; NbExp=3; IntAct=EBI-14513896, EBI-724076;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF187987; AAF04103.1; -; mRNA.
DR EMBL; AC035150; AAF63029.1; -; Genomic_DNA.
DR EMBL; AK314207; BAG36883.1; -; mRNA.
DR EMBL; AC006213; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KF459580; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471126; EAW57237.1; -; Genomic_DNA.
DR EMBL; BC111981; AAI11982.1; -; mRNA.
DR EMBL; BC113426; AAI13427.1; -; mRNA.
DR CCDS; CCDS12633.1; -.
DR RefSeq; NP_001284517.1; NM_001297588.1.
DR RefSeq; NP_001284518.1; NM_001297589.1.
DR RefSeq; NP_037491.2; NM_013359.2.
DR RefSeq; XP_016882721.1; XM_017027232.1.
DR AlphaFoldDB; Q9UK13; -.
DR SMR; Q9UK13; -.
DR BioGRID; 113454; 10.
DR IntAct; Q9UK13; 6.
DR STRING; 9606.ENSP00000251269; -.
DR iPTMnet; Q9UK13; -.
DR PhosphoSitePlus; Q9UK13; -.
DR BioMuta; ZNF221; -.
DR DMDM; 311033541; -.
DR EPD; Q9UK13; -.
DR jPOST; Q9UK13; -.
DR MassIVE; Q9UK13; -.
DR PaxDb; Q9UK13; -.
DR PeptideAtlas; Q9UK13; -.
DR PRIDE; Q9UK13; -.
DR ProteomicsDB; 84704; -.
DR Antibodypedia; 815; 43 antibodies from 12 providers.
DR DNASU; 7638; -.
DR Ensembl; ENST00000251269.9; ENSP00000251269.4; ENSG00000159905.15.
DR Ensembl; ENST00000587682.6; ENSP00000467367.1; ENSG00000159905.15.
DR Ensembl; ENST00000592350.5; ENSP00000467446.1; ENSG00000159905.15.
DR Ensembl; ENST00000622072.1; ENSP00000477876.1; ENSG00000159905.15.
DR GeneID; 7638; -.
DR KEGG; hsa:7638; -.
DR MANE-Select; ENST00000587682.6; ENSP00000467367.1; NM_001297588.2; NP_001284517.1.
DR UCSC; uc002oxx.3; human.
DR UCSC; uc060zov.1; human.
DR CTD; 7638; -.
DR GeneCards; ZNF221; -.
DR HGNC; HGNC:13014; ZNF221.
DR HPA; ENSG00000159905; Low tissue specificity.
DR neXtProt; NX_Q9UK13; -.
DR OpenTargets; ENSG00000159905; -.
DR PharmGKB; PA37593; -.
DR VEuPathDB; HostDB:ENSG00000159905; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000160225; -.
DR InParanoid; Q9UK13; -.
DR OMA; LWMMKTT; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9UK13; -.
DR PathwayCommons; Q9UK13; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q9UK13; -.
DR BioGRID-ORCS; 7638; 15 hits in 1060 CRISPR screens.
DR GenomeRNAi; 7638; -.
DR Pharos; Q9UK13; Tdark.
DR PRO; PR:Q9UK13; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9UK13; protein.
DR Bgee; ENSG00000159905; Expressed in buccal mucosa cell and 117 other tissues.
DR ExpressionAtlas; Q9UK13; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 10.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 15.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 8.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 15.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 15.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..617
FT /note="Zinc finger protein 221"
FT /id="PRO_0000047462"
FT DOMAIN 30..100
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 170..192
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 198..220
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 226..248
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 254..276
FT /note="C2H2-type 4; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 282..304
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 310..332
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 338..360
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 366..388
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 394..416
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 422..444
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 450..472
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 478..500
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 506..528
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 534..556
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 562..584
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT VARIANT 165
FT /note="V -> M (in dbSNP:rs16976937)"
FT /id="VAR_033557"
FT VARIANT 179
FT /note="F -> I (in dbSNP:rs454301)"
FT /evidence="ECO:0000269|PubMed:12743021,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.5"
FT /id="VAR_033558"
FT VARIANT 256
FT /note="C -> R (in dbSNP:rs439676)"
FT /evidence="ECO:0000269|PubMed:12743021,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.5"
FT /id="VAR_033559"
FT VARIANT 337
FT /note="P -> A (in dbSNP:rs435590)"
FT /id="VAR_024201"
FT VARIANT 519
FT /note="S -> T (in dbSNP:rs365745)"
FT /evidence="ECO:0000269|PubMed:12743021,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.5"
FT /id="VAR_024202"
FT VARIANT 557
FT /note="G -> R (in dbSNP:rs366111)"
FT /evidence="ECO:0000269|PubMed:12743021,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.5"
FT /id="VAR_024203"
FT CONFLICT 14
FT /note="C -> Y (in Ref. 1; AAF04103)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 617 AA; 71197 MW; 0E27FCDEDA0EC1D4 CRC64;
MISPSLELLH SGLCKFPEVE GKMTTFKEAV TFKDVAVVFT EEELGLLDPA QRKLYRDVML
ENFRNLLSVG NQPFHQDTFH FLGKEKFWKM KTTSQREGNS GGKIQIEMET VPEAGPHEEW
SCQQIWEQIA SDLTRSQNSI RNSSQFFKEG DVPCQIEARL SISHVQQKPY RCNECKQSFS
DVSVFDLHQQ SHSGEKSHTC GECGKSFCYS PALHIHQRVH MGEKCYKCDV CGKEFNQSSH
LQTHQRVHTG EKPFKCGQCG KGFHSRSALN VHCKLHTGEK PYNCEECGKA FIHDSQLQEH
QRIHTGEKPF KCDICGKSFR VRSRLNRHSM VHTGEKPFRC DTCGKNFRQR SALNSHSMVH
IEEKPYKCEQ CGKGFICRRD FCKHQMVHTG EKPYNCKECG KTFRWSSCLL NHQQVHSGQK
SFKCEECGKG FYTNSRRSSH QRSHNGEKPY NCEECGKDYK RRLDLEFHQR VHTGERPYNC
KECGKSFGWA SCLLKHQRLH SGEKPFKCEE CGKRFTQSSQ LHSHQTCHTG EKLYKCEQCE
KGYNSKFNLD MHQRVHGGER PYNCKECGKS FGWASCLLKH QRLHSGEKPL KSGVWEEIYS
EFTASFTSVS LCGRKAI
