ZN225_HUMAN
ID ZN225_HUMAN Reviewed; 706 AA.
AC Q9UK10; A8K8S2; Q53F12; Q9NS46; Q9UID8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Zinc finger protein 225;
GN Name=ZNF225;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12743021; DOI=10.1101/gr.963903;
RA Shannon M., Hamilton A.T., Gordon L., Branscomb E., Stubbs L.;
RT "Differential expansion of zinc-finger transcription factor loci in
RT homologous human and mouse gene clusters.";
RL Genome Res. 13:1097-1110(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Synovial cell;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-61, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q9UK10; O76024: WFS1; NbExp=3; IntAct=EBI-21856539, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF187991; AAF04107.1; -; mRNA.
DR EMBL; AK223477; BAD97197.1; -; mRNA.
DR EMBL; AK292437; BAF85126.1; -; mRNA.
DR EMBL; AC021092; AAF24967.1; -; Genomic_DNA.
DR EMBL; AC074331; AAF88105.1; -; Genomic_DNA.
DR EMBL; CH471126; EAW57255.1; -; Genomic_DNA.
DR EMBL; BC108912; AAI08913.1; -; mRNA.
DR CCDS; CCDS46100.1; -.
DR RefSeq; NP_001308614.1; NM_001321685.1.
DR RefSeq; NP_037494.2; NM_013362.3.
DR RefSeq; XP_011525587.1; XM_011527285.2.
DR RefSeq; XP_011525588.1; XM_011527286.2.
DR AlphaFoldDB; Q9UK10; -.
DR SMR; Q9UK10; -.
DR BioGRID; 113551; 5.
DR IntAct; Q9UK10; 3.
DR STRING; 9606.ENSP00000262894; -.
DR iPTMnet; Q9UK10; -.
DR PhosphoSitePlus; Q9UK10; -.
DR BioMuta; ZNF225; -.
DR DMDM; 110825755; -.
DR EPD; Q9UK10; -.
DR jPOST; Q9UK10; -.
DR MassIVE; Q9UK10; -.
DR PaxDb; Q9UK10; -.
DR PeptideAtlas; Q9UK10; -.
DR PRIDE; Q9UK10; -.
DR ProteomicsDB; 84701; -.
DR Antibodypedia; 49032; 136 antibodies from 22 providers.
DR DNASU; 7768; -.
DR Ensembl; ENST00000262894.11; ENSP00000262894.5; ENSG00000256294.8.
DR Ensembl; ENST00000590612.1; ENSP00000468686.1; ENSG00000256294.8.
DR GeneID; 7768; -.
DR KEGG; hsa:7768; -.
DR MANE-Select; ENST00000262894.11; ENSP00000262894.5; NM_013362.4; NP_037494.2.
DR UCSC; uc002oyj.2; human.
DR CTD; 7768; -.
DR GeneCards; ZNF225; -.
DR HGNC; HGNC:13018; ZNF225.
DR HPA; ENSG00000256294; Low tissue specificity.
DR neXtProt; NX_Q9UK10; -.
DR OpenTargets; ENSG00000256294; -.
DR PharmGKB; PA37597; -.
DR VEuPathDB; HostDB:ENSG00000256294; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000160225; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; Q9UK10; -.
DR OMA; GRKCKKF; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9UK10; -.
DR TreeFam; TF341885; -.
DR PathwayCommons; Q9UK10; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q9UK10; -.
DR BioGRID-ORCS; 7768; 14 hits in 1104 CRISPR screens.
DR GeneWiki; ZNF225; -.
DR GenomeRNAi; 7768; -.
DR Pharos; Q9UK10; Tdark.
DR PRO; PR:Q9UK10; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9UK10; protein.
DR Bgee; ENSG00000256294; Expressed in calcaneal tendon and 132 other tissues.
DR ExpressionAtlas; Q9UK10; baseline and differential.
DR Genevisible; Q9UK10; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 13.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 17.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 11.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 17.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 18.
PE 1: Evidence at protein level;
KW DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc;
KW Zinc-finger.
FT CHAIN 1..706
FT /note="Zinc finger protein 225"
FT /id="PRO_0000047466"
FT DOMAIN 8..78
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 176..198
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 204..226
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 232..254
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 260..282
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 288..310
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 316..338
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 344..366
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 372..394
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 400..422
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 428..450
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 456..478
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 484..506
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 512..534
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 540..562
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 568..590
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 596..618
FT /note="C2H2-type 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 624..646
FT /note="C2H2-type 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 652..674
FT /note="C2H2-type 18; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT CROSSLNK 61
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VARIANT 50
FT /note="Q -> R (in dbSNP:rs34863330)"
FT /id="VAR_033560"
FT VARIANT 679
FT /note="T -> S (in dbSNP:rs16978738)"
FT /id="VAR_033561"
FT CONFLICT 144..147
FT /note="RQKP -> KTET (in Ref. 1; AAF04107)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="L -> Q (in Ref. 1; AAF04107)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 706 AA; 82471 MW; 4C357A3AB1E32FB2 CRC64;
MTTLKEAVTF KDVAVVFTEE ELRLLDLAQR KLYREVMLEN FRNLLSVGHQ SLHRDTFHFL
KEEKFWMMET ATQREGNLGG KIQMEMETVS ESGTHEGLFS HQTWEQISSD LTRFQDSMVN
SFQFSKQDDM PCQVDAGLSI IHVRQKPSEG RTCKKSFSDV SVLDLHQQLQ SREKSHTCDE
CGKSFCYSSA LRIHQRVHMG EKLYNCDVCG KEFNQSSHLQ IHQRIHTGEK PFKCEQCGKG
FSRRSGLYVH RKLHTGVKPH ICEKCGKAFI HDSQLQEHQR IHTGEKPFKC DICCKSFRSR
ANLNRHSMVH MREKPFRCDT CGKSFGLKSA LNSHRMVHTG EKRYKCEECG KRFIYRQDLY
KHQIDHTGEK PYNCKECGKS FRWASGLSRH VRVHSGETTF KCEECGKGFY TNSQRYSHQR
AHSGEKPYRC EECGKGYKRR LDLDFHQRVH RGEKPYNCKE CGKSFGWASC LLNHQRIHSG
EKPFKCEECG KRFTQNSQLY THRRVHSGEK PFKCEECGKR FTQNSQLYSH RRVHTGVKPY
KCEECGKGFN SKFNLDMHQR VHTGERPYNC KECGKSFSRA SSILNHKRLH GDEKPFKCEE
CGKRFTENSQ LHSHQRVHTG EKPYKCEKCG KSFRWASTHL THQRLHSREK LLQCEDCGKS
IVHSSCLKDQ QRDQSGEKTS KCEDCGKRYK RRLNLDTLLS LFLNDT
