ZN227_HUMAN
ID ZN227_HUMAN Reviewed; 799 AA.
AC Q86WZ6; B3KRU7; B7Z5P9;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Zinc finger protein 227;
GN Name=ZNF227;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q86WZ6; Q8NHQ1: CEP70; NbExp=7; IntAct=EBI-2799529, EBI-739624;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86WZ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86WZ6-2; Sequence=VSP_056544;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK092253; BAG52509.1; -; mRNA.
DR EMBL; AK299266; BAH12985.1; -; mRNA.
DR EMBL; AK316048; BAH14419.1; -; mRNA.
DR EMBL; AC138470; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471126; EAW57261.1; -; Genomic_DNA.
DR EMBL; BC047570; AAH47570.1; -; mRNA.
DR CCDS; CCDS12636.1; -. [Q86WZ6-1]
DR CCDS; CCDS74388.1; -. [Q86WZ6-2]
DR RefSeq; NP_001276095.1; NM_001289166.1. [Q86WZ6-1]
DR RefSeq; NP_001276096.1; NM_001289167.1. [Q86WZ6-2]
DR RefSeq; NP_001276097.1; NM_001289168.1. [Q86WZ6-2]
DR RefSeq; NP_001276098.1; NM_001289169.1. [Q86WZ6-2]
DR RefSeq; NP_001276099.1; NM_001289170.1.
DR RefSeq; NP_872296.1; NM_182490.2. [Q86WZ6-1]
DR RefSeq; XP_005259289.1; XM_005259232.2. [Q86WZ6-1]
DR RefSeq; XP_011525591.1; XM_011527289.2. [Q86WZ6-1]
DR RefSeq; XP_016882755.1; XM_017027266.1. [Q86WZ6-1]
DR RefSeq; XP_016882757.1; XM_017027268.1. [Q86WZ6-2]
DR RefSeq; XP_016882758.1; XM_017027269.1. [Q86WZ6-2]
DR RefSeq; XP_016882759.1; XM_017027270.1. [Q86WZ6-2]
DR RefSeq; XP_016882760.1; XM_017027271.1. [Q86WZ6-2]
DR AlphaFoldDB; Q86WZ6; -.
DR SMR; Q86WZ6; -.
DR BioGRID; 113553; 72.
DR IntAct; Q86WZ6; 11.
DR MINT; Q86WZ6; -.
DR STRING; 9606.ENSP00000482749; -.
DR iPTMnet; Q86WZ6; -.
DR PhosphoSitePlus; Q86WZ6; -.
DR BioMuta; ZNF227; -.
DR DMDM; 55976727; -.
DR EPD; Q86WZ6; -.
DR jPOST; Q86WZ6; -.
DR MassIVE; Q86WZ6; -.
DR MaxQB; Q86WZ6; -.
DR PaxDb; Q86WZ6; -.
DR PeptideAtlas; Q86WZ6; -.
DR PRIDE; Q86WZ6; -.
DR ProteomicsDB; 6708; -.
DR ProteomicsDB; 70216; -. [Q86WZ6-1]
DR Antibodypedia; 17695; 162 antibodies from 24 providers.
DR DNASU; 7770; -.
DR Ensembl; ENST00000313040.12; ENSP00000321049.6; ENSG00000131115.16. [Q86WZ6-1]
DR Ensembl; ENST00000391961.6; ENSP00000375823.2; ENSG00000131115.16. [Q86WZ6-2]
DR Ensembl; ENST00000589005.5; ENSP00000467577.1; ENSG00000131115.16. [Q86WZ6-2]
DR Ensembl; ENST00000621083.4; ENSP00000482749.1; ENSG00000131115.16. [Q86WZ6-1]
DR GeneID; 7770; -.
DR KEGG; hsa:7770; -.
DR MANE-Select; ENST00000313040.12; ENSP00000321049.6; NM_182490.3; NP_872296.1.
DR UCSC; uc002oyu.5; human. [Q86WZ6-1]
DR CTD; 7770; -.
DR GeneCards; ZNF227; -.
DR HGNC; HGNC:13020; ZNF227.
DR HPA; ENSG00000131115; Low tissue specificity.
DR neXtProt; NX_Q86WZ6; -.
DR OpenTargets; ENSG00000131115; -.
DR PharmGKB; PA37599; -.
DR VEuPathDB; HostDB:ENSG00000131115; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162819; -.
DR HOGENOM; CLU_002678_31_4_1; -.
DR InParanoid; Q86WZ6; -.
DR OMA; ICEDFMK; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q86WZ6; -.
DR TreeFam; TF350845; -.
DR PathwayCommons; Q86WZ6; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q86WZ6; -.
DR BioGRID-ORCS; 7770; 11 hits in 1099 CRISPR screens.
DR ChiTaRS; ZNF227; human.
DR GenomeRNAi; 7770; -.
DR Pharos; Q86WZ6; Tdark.
DR PRO; PR:Q86WZ6; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q86WZ6; protein.
DR Bgee; ENSG00000131115; Expressed in secondary oocyte and 171 other tissues.
DR ExpressionAtlas; Q86WZ6; baseline and differential.
DR Genevisible; Q86WZ6; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 16.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 18.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 11.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 18.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 19.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..799
FT /note="Zinc finger protein 227"
FT /id="PRO_0000047468"
FT DOMAIN 23..94
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 250..272
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 269..291
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 324..346
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 352..374
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 380..402
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 408..430
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 436..458
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 464..486
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 492..514
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 520..542
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 548..570
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 576..598
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 604..626
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 632..654
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 660..682
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 688..710
FT /note="C2H2-type 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 716..738
FT /note="C2H2-type 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 744..766
FT /note="C2H2-type 18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 772..794
FT /note="C2H2-type 19"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT VAR_SEQ 1..51
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056544"
SQ SEQUENCE 799 AA; 92033 MW; 8D3506F40D71FA8A CRC64;
MPSQNYDLPQ KKQEKMTKFQ EAVTFKDVAV VFSREELRLL DLTQRKLYRD VMVENFKNLV
AVGHLPFQPD MVSQLEAEEK LWMMETETQR SSKHQNKMET LQKFALKYLS NQELSCWQIW
KQVASELTRC LQGKSSQLLQ GDSIQVSENE NNIMNPKGDS SIYIENQEFP FWRTQHSCGN
TYLSESQIQS RGKQIDVKNN LQIHEDFMKK SPFHEHIKTD TEPKPCKGNE YGKIISDGSN
QKLPLGEKPH PCGECGRGFS YSPRLPLHPN VHTGEKCFSQ SSHLRTHQRI HPGEKLNRCH
ESGDCFNKSS FHSYQSNHTG EKSYRCDSCG KGFSSSTGLI IHYRTHTGEK PYKCEECGKC
FSQSSNFQCH QRVHTEEKPY KCEECGKGFG WSVNLRVHQR VHRGEKPYKC EECGKGFTQA
AHFHIHQRVH TGEKPYKCDV CGKGFSHNSP LICHRRVHTG EKPYKCEACG KGFTRNTDLH
IHFRVHTGEK PYKCKECGKG FSQASNLQVH QNVHTGEKRF KCETCGKGFS QSSKLQTHQR
VHTGEKPYRC DVCGKDFSYS SNLKLHQVIH TGEKPYKCEE CGKGFSWRSN LHAHQRVHSG
EKPYKCEQCD KSFSQAIDFR VHQRVHTGEK PYKCGVCGKG FSQSSGLQSH QRVHTGEKPY
KCDVCGKGFR YSSQFIYHQR GHTGEKPYKC EECGKGFGRS LNLRHHQRVH TGEKPHICEE
CGKAFSLPSN LRVHLGVHTR EKLFKCEECG KGFSQSARLE AHQRVHTGEK PYKCDICDKD
FRHRSRLTYH QKVHTGKKL
