ZN256_HUMAN
ID ZN256_HUMAN Reviewed; 627 AA.
AC Q9Y2P7; B2RA92; Q53Y85; Q9BV71;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Zinc finger protein 256;
DE AltName: Full=Bone marrow zinc finger 3;
DE Short=BMZF-3;
GN Name=ZNF256; Synonyms=BMZF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Bone marrow;
RX PubMed=10585455; DOI=10.1074/jbc.274.50.35741;
RA Han Z.-G., Zhang Q.-H., Ye M., Kan L.-X., Gu B.-W., He K.-L., Shi S.-L.,
RA Zhou J., Fu G., Mao M., Chen S.-J., Yu L., Chen Z.;
RT "Molecular cloning of six novel Kruppel-like zinc finger genes from
RT hematopoietic cells and identification of a novel transregulatory domain
RT KRNB.";
RL J. Biol. Chem. 274:35741-35748(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION, INDUCTION, SUBCELLULAR LOCATION, DOMAIN, AND INTERACTION WITH
RP TRIM28.
RX PubMed=18060868; DOI=10.1016/j.bbrc.2007.11.118;
RA Suzuki C., Murakumo Y., Kawase Y., Sato T., Morinaga T., Fukuda N.,
RA Enomoto A., Ichihara M., Takahashi M.;
RT "A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor
RT associated with KAP-1.";
RL Biochem. Biophys. Res. Commun. 366:226-232(2008).
CC -!- FUNCTION: Transcriptional repressor that plays a role in cell
CC proliferation. Requires TRIM28 for its activity.
CC {ECO:0000269|PubMed:18060868}.
CC -!- SUBUNIT: Interacts with TRIM28. {ECO:0000269|PubMed:18060868}.
CC -!- INTERACTION:
CC Q9Y2P7; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-12223065, EBI-10172290;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18060868}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y2P7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y2P7-2; Sequence=VSP_037721;
CC -!- INDUCTION: By GDNF/glial cell line-derived neurotrophic factor.
CC {ECO:0000269|PubMed:18060868}.
CC -!- DOMAIN: The KRAB domain mediates interaction with TRIM28 and is
CC required for transcriptional repressor activity.
CC {ECO:0000269|PubMed:18060868}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF067165; AAD32449.1; -; mRNA.
DR EMBL; BT006853; AAP35499.1; -; mRNA.
DR EMBL; AK314094; BAG36789.1; -; mRNA.
DR EMBL; AC008969; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC010326; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC001438; AAH01438.2; -; mRNA.
DR CCDS; CCDS12966.1; -. [Q9Y2P7-1]
DR RefSeq; NP_005764.2; NM_005773.2. [Q9Y2P7-1]
DR RefSeq; XP_016881627.1; XM_017026138.1.
DR AlphaFoldDB; Q9Y2P7; -.
DR SMR; Q9Y2P7; -.
DR BioGRID; 115474; 5.
DR IntAct; Q9Y2P7; 2.
DR STRING; 9606.ENSP00000282308; -.
DR iPTMnet; Q9Y2P7; -.
DR PhosphoSitePlus; Q9Y2P7; -.
DR BioMuta; ZNF256; -.
DR DMDM; 254763378; -.
DR MassIVE; Q9Y2P7; -.
DR MaxQB; Q9Y2P7; -.
DR PaxDb; Q9Y2P7; -.
DR PeptideAtlas; Q9Y2P7; -.
DR PRIDE; Q9Y2P7; -.
DR ProteomicsDB; 85855; -. [Q9Y2P7-1]
DR ProteomicsDB; 85856; -. [Q9Y2P7-2]
DR Antibodypedia; 33319; 125 antibodies from 23 providers.
DR DNASU; 10172; -.
DR Ensembl; ENST00000282308.4; ENSP00000282308.2; ENSG00000152454.4. [Q9Y2P7-1]
DR GeneID; 10172; -.
DR KEGG; hsa:10172; -.
DR MANE-Select; ENST00000282308.4; ENSP00000282308.2; NM_005773.3; NP_005764.2.
DR UCSC; uc002qqu.4; human. [Q9Y2P7-1]
DR CTD; 10172; -.
DR GeneCards; ZNF256; -.
DR HGNC; HGNC:13049; ZNF256.
DR HPA; ENSG00000152454; Low tissue specificity.
DR MIM; 606956; gene.
DR neXtProt; NX_Q9Y2P7; -.
DR OpenTargets; ENSG00000152454; -.
DR PharmGKB; PA37627; -.
DR VEuPathDB; HostDB:ENSG00000152454; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000154734; -.
DR HOGENOM; CLU_002678_55_2_1; -.
DR InParanoid; Q9Y2P7; -.
DR OMA; VEHQGTH; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9Y2P7; -.
DR TreeFam; TF339848; -.
DR PathwayCommons; Q9Y2P7; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q9Y2P7; -.
DR BioGRID-ORCS; 10172; 13 hits in 1091 CRISPR screens.
DR GenomeRNAi; 10172; -.
DR Pharos; Q9Y2P7; Tdark.
DR PRO; PR:Q9Y2P7; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9Y2P7; protein.
DR Bgee; ENSG00000152454; Expressed in oocyte and 119 other tissues.
DR ExpressionAtlas; Q9Y2P7; baseline and differential.
DR Genevisible; Q9Y2P7; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 14.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 15.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 9.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 15.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 15.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..627
FT /note="Zinc finger protein 256"
FT /id="PRO_0000047489"
FT DOMAIN 14..96
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 90..112
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 239..261
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 267..289
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 295..317
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 323..345
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 351..373
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 379..401
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 407..429
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 435..457
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 463..485
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 491..513
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 519..541
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 547..569
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 575..597
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 603..625
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 55..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..153
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10585455,
FT ECO:0000303|PubMed:14702039, ECO:0000303|Ref.2"
FT /id="VSP_037721"
FT VARIANT 304
FT /note="F -> I (in dbSNP:rs953619)"
FT /id="VAR_058326"
FT CONFLICT 254
FT /note="L -> P (in Ref. 3; BAG36789)"
FT /evidence="ECO:0000305"
FT CONFLICT 552
FT /note="C -> F (in Ref. 3; BAG36789)"
FT /evidence="ECO:0000305"
FT CONFLICT 574
FT /note="P -> L (in Ref. 1; AAD32449)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 627 AA; 71871 MW; F03506B03E4A6E86 CRC64;
MAAAELTAPA QGIVTFEDVA VYFSWKEWGL LDEAQKCLYH DVMLENLTLT TSLGGSGAGD
EEAPYQQSTS PQRVSQVRIP KALPSPQKTN PCEICGPVLR QILHLVEHQG THHGQKLYTD
GACRKQLQFT AYLHQHQKQH VGQKHFRSNG GRDMFLSSCT FEVSGKPFTC KEVGKDFLVR
SRFLQQQAAH TRKKSNRTKS AVAFHSVKNH YNWGECVKAF SYKHVRVQHQ GDLIRERSYM
CSECGKSFST SCSLSDHLRV HTSEKPYTCG ECGKSYRQSS SLITHRRIHT GVRPHQCDEC
GKLFNRKYDL LIHQRVHTGE RPYKCSECGK SFSHSSSLIT HQRIHTGMRP YECSECGKSF
IHSSSLITHQ RVHTGTRPYM CSECGKSFSQ SCHLIKHRRL HIGEGPYECS ECGKLFTYRS
RFFQHQRVHT GVRSHECHEC GKLFSRKFDL IVHERVHTGE RPYECSECGK SFTCKSYLIS
HWKVHTGARP YECGECGKSF THSSTLLQHQ RVHTGERPYE CNECGKFFSQ SSSLIRHRRS
HTGERPYECS ECWKSFSNHS SLVKHRRVHT GERPYECSEC GKSFSQSSNL TNHQRIHSGE
RPYECSDCGK FFTFNSNLLK HQNVHKG
