ZN296_MOUSE
ID ZN296_MOUSE Reviewed; 445 AA.
AC E9Q6W4; Q4FZI6; Q9D7X0; Q9EPM0;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Zinc finger protein 296 {ECO:0000312|MGI:MGI:1926956};
DE AltName: Full=Hepatocellular carcinoma-associated antigen MHCA108 {ECO:0000303|Ref.1};
GN Name=Znf296 {ECO:0000250|UniProtKB:Q8WUU4};
GN Synonyms=Zfp296 {ECO:0000312|MGI:MGI:1926956};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1] {ECO:0000312|EMBL:AAN76749.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ {ECO:0000312|EMBL:AAN76749.1};
RA Dong X., Su Y., Chen W.;
RT "MHCA108, a mouse hepatocellular carcinoma-associated antigen.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:BAB25873.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAB25873.1};
RC TISSUE=Stomach {ECO:0000312|EMBL:BAB25873.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4] {ECO:0000312|EMBL:EDL23160.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AAH99454.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH99454.1};
RC TISSUE=Oocyte {ECO:0000312|EMBL:AAH99454.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6] {ECO:0000312|EMBL:CAC18551.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 83-445, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=129 {ECO:0000312|EMBL:CAC18551.1};
RX PubMed=11063263; DOI=10.1007/s003350010182;
RA Dear T.N.;
RT "Cloning, structure, expression analysis, and assignment to mouse
RT chromosome 7 of the gene Zfp296 encoding a zinc finger protein.";
RL Mamm. Genome 11:1037-1039(2000).
RN [7] {ECO:0000305}
RP FUNCTION, INTERACTION WITH KLF4, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=24161396; DOI=10.1016/j.bbrc.2013.10.073;
RA Fujii Y., Kakegawa M., Koide H., Akagi T., Yokota T.;
RT "Zfp296 is a novel Klf4-interacting protein and functions as a negative
RT regulator.";
RL Biochem. Biophys. Res. Commun. 441:411-417(2013).
CC -!- FUNCTION: May be a transcriptional corepressor with KLF4.
CC {ECO:0000269|PubMed:24161396}.
CC -!- SUBUNIT: Interacts with KLF4. {ECO:0000269|PubMed:24161396}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24161396}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in testis and embryonic stem
CC cells. {ECO:0000269|PubMed:11063263}.
CC -!- DEVELOPMENTAL STAGE: In testis, detected in condensing spermatids but
CC not at earlier stages of spermatogenesis (PubMed:11063263). In
CC embryonic stem cells, expressed in undifferentiated cells and down-
CC regulated upon differentiation (PubMed:24161396).
CC {ECO:0000269|PubMed:11063263, ECO:0000269|PubMed:24161396}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN76749.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB25873.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAC18551.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EDL23160.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF450095; AAN76749.1; ALT_FRAME; mRNA.
DR EMBL; AK008748; BAB25873.1; ALT_FRAME; mRNA.
DR EMBL; AC149052; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466639; EDL23160.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC099454; AAH99454.1; -; mRNA.
DR EMBL; AJ278340; CAC18551.1; ALT_INIT; mRNA.
DR CCDS; CCDS52060.1; -.
DR RefSeq; NP_071854.2; NM_022409.2.
DR AlphaFoldDB; E9Q6W4; -.
DR STRING; 10090.ENSMUSP00000104093; -.
DR iPTMnet; E9Q6W4; -.
DR PhosphoSitePlus; E9Q6W4; -.
DR EPD; E9Q6W4; -.
DR PaxDb; E9Q6W4; -.
DR PeptideAtlas; E9Q6W4; -.
DR PRIDE; E9Q6W4; -.
DR ProteomicsDB; 275280; -.
DR Antibodypedia; 17828; 127 antibodies from 19 providers.
DR DNASU; 63872; -.
DR Ensembl; ENSMUST00000108453; ENSMUSP00000104093; ENSMUSG00000011267.
DR GeneID; 63872; -.
DR KEGG; mmu:63872; -.
DR UCSC; uc009fmj.2; mouse.
DR CTD; 63872; -.
DR MGI; MGI:1926956; Zfp296.
DR VEuPathDB; HostDB:ENSMUSG00000011267; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000160667; -.
DR HOGENOM; CLU_049038_0_0_1; -.
DR InParanoid; E9Q6W4; -.
DR OMA; LGARNPW; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; E9Q6W4; -.
DR TreeFam; TF318131; -.
DR BioGRID-ORCS; 63872; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Zfp296; mouse.
DR PRO; PR:E9Q6W4; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; E9Q6W4; protein.
DR Bgee; ENSMUSG00000011267; Expressed in primary oocyte and 76 other tissues.
DR Genevisible; E9Q6W4; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IEP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW Isopeptide bond; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Ubl conjugation; Zinc;
KW Zinc-finger.
FT CHAIN 1..445
FT /note="Zinc finger protein 296"
FT /id="PRO_0000436774"
FT ZN_FING 138..161
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 212..234
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 240..262
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 360..382
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 388..410
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 418..441
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..356
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 31
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8WUU4"
FT CONFLICT 88
FT /note="T -> I (in Ref. 5; AAH99454)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="L -> P (in Ref. 1; AAN76749 and 2; BAB25873)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 445 AA; 47821 MW; 14D51519D2B028AD CRC64;
MSRRKAGRVP RRVDPDTDTD IEMPDLVMDV KPDLDLRSLA QGPWIARDMP ISDVKRQLQT
ASRPLGAPST CAPRMPLSSK SSDRQPWTDK HPDLLTCGRC GKIFPLGAII AFMDHKKQGC
QLLQVSDPIS ESKELKALSC LQCGRQYTSP WKLLCHAQWD HGLCIYQTQH LDTPEAPLLG
LAEVAAAMSA VAVVAPVESK PPPVSSAARR SPTCDVCKKT LSSFSNLKVH MRSHTGERPY
SCDQCSYACA QSSKLNRHKK THRQLAPGSP STSASSRGVS PAAPPEPAAY AAAPASTLPS
QTVEKAGAAA TAGVQEPGAP GSGAQGGPGF VGWGAPAKVE RTDPVKIEKT APRKSHGPGG
KCEFCGKSFT NSSNLTVHRR SHTGERPYTC DQCPYACAQS SKLNRHRRTH GLGTGKTVKC
PHCLVPFGLQ ATLDKHLRQK HPEMA
