ZN302_HUMAN
ID ZN302_HUMAN Reviewed; 478 AA.
AC Q9NR11; Q658J3; Q9BZD8; Q9P0J4;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Zinc finger protein 302;
DE AltName: Full=Zinc finger protein 135-like;
DE AltName: Full=Zinc finger protein 140-like;
DE AltName: Full=Zinc finger protein 327;
GN Name=ZNF302; Synonyms=ZNF135L, ZNF140L, ZNF327;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Mao Y.-M., Xie Y., Zhao W., Kang Y., Lin S.-R., Sun Z.-H., Zhou Z.;
RT "Molecular cloning and characterization of a full-length cDNA encoding a
RT novel KRAB type zinc finger protein.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Mao Y.-M., Xie Y., Wang Z., Ying K., Lin S.-R., Deng Y.-F., Sun Z.-H.;
RT "A novel KRAB ZFP gene involved in hepatic cancer.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Adrenal gland;
RA Gu J., Fu S., Ren S., Jin W., Gu Y., Huang Q., Dong H., Yu Y., Fu G.,
RA Wang Y., Chen Z., Han Z.;
RT "A novel gene expressed in human adrenal gland.";
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-183, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25218447; DOI=10.1038/nsmb.2890;
RA Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M.,
RA Vertegaal A.C.;
RT "Uncovering global SUMOylation signaling networks in a site-specific
RT manner.";
RL Nat. Struct. Mol. Biol. 21:927-936(2014).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-183, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q9NR11; Q8NHQ1: CEP70; NbExp=4; IntAct=EBI-3916142, EBI-739624;
CC Q9NR11-2; P14136: GFAP; NbExp=3; IntAct=EBI-12988373, EBI-744302;
CC Q9NR11-2; P42858: HTT; NbExp=3; IntAct=EBI-12988373, EBI-466029;
CC Q9NR11-2; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-12988373, EBI-1055254;
CC Q9NR11-2; P60371: KRTAP10-6; NbExp=3; IntAct=EBI-12988373, EBI-12012928;
CC Q9NR11-2; P07196: NEFL; NbExp=3; IntAct=EBI-12988373, EBI-475646;
CC Q9NR11-2; Q96CV9: OPTN; NbExp=3; IntAct=EBI-12988373, EBI-748974;
CC Q9NR11-2; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-12988373, EBI-396669;
CC Q9NR11-2; O76024: WFS1; NbExp=3; IntAct=EBI-12988373, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NR11-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NR11-2; Sequence=VSP_006914;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF265236; AAF74775.1; -; mRNA.
DR EMBL; AF326206; AAK11224.1; -; mRNA.
DR EMBL; AF155656; AAF67475.1; -; mRNA.
DR EMBL; AL834318; CAD38987.1; -; mRNA.
DR EMBL; BC024176; AAH24176.1; -; mRNA.
DR CCDS; CCDS46042.1; -. [Q9NR11-2]
DR RefSeq; NP_001012320.1; NM_001012320.2. [Q9NR11-2]
DR RefSeq; NP_001276110.1; NM_001289181.1.
DR RefSeq; NP_001276111.1; NM_001289182.1.
DR RefSeq; NP_001276112.1; NM_001289183.1. [Q9NR11-2]
DR RefSeq; NP_001276113.1; NM_001289184.1.
DR RefSeq; NP_001276114.1; NM_001289185.1.
DR RefSeq; NP_001276115.1; NM_001289186.1. [Q9NR11-2]
DR RefSeq; NP_001276116.1; NM_001289187.1. [Q9NR11-2]
DR RefSeq; NP_001276117.1; NM_001289188.1.
DR RefSeq; NP_001276118.1; NM_001289189.1.
DR RefSeq; NP_001276119.1; NM_001289190.1.
DR RefSeq; NP_001276120.1; NM_001289191.1.
DR RefSeq; NP_001276121.1; NM_001289192.1.
DR RefSeq; NP_060913.2; NM_018443.3. [Q9NR11-2]
DR RefSeq; NP_061145.2; NM_018675.2.
DR AlphaFoldDB; Q9NR11; -.
DR SMR; Q9NR11; -.
DR BioGRID; 120987; 20.
DR IntAct; Q9NR11; 22.
DR MINT; Q9NR11; -.
DR iPTMnet; Q9NR11; -.
DR PhosphoSitePlus; Q9NR11; -.
DR BioMuta; ZNF302; -.
DR DMDM; 23397010; -.
DR EPD; Q9NR11; -.
DR MassIVE; Q9NR11; -.
DR MaxQB; Q9NR11; -.
DR PaxDb; Q9NR11; -.
DR PeptideAtlas; Q9NR11; -.
DR PRIDE; Q9NR11; -.
DR ProteomicsDB; 82242; -. [Q9NR11-1]
DR ProteomicsDB; 82243; -. [Q9NR11-2]
DR Antibodypedia; 29194; 107 antibodies from 16 providers.
DR DNASU; 55900; -.
DR Ensembl; ENST00000423823.6; ENSP00000405219.2; ENSG00000089335.22. [Q9NR11-2]
DR Ensembl; ENST00000457781.6; ENSP00000391067.2; ENSG00000089335.22. [Q9NR11-2]
DR Ensembl; ENST00000505242.6; ENSP00000421028.1; ENSG00000089335.22. [Q9NR11-2]
DR GeneID; 55900; -.
DR KEGG; hsa:55900; -.
DR MANE-Select; ENST00000505242.6; ENSP00000421028.1; NM_001289187.2; NP_001276116.1. [Q9NR11-2]
DR UCSC; uc002nvp.3; human. [Q9NR11-1]
DR CTD; 55900; -.
DR DisGeNET; 55900; -.
DR GeneCards; ZNF302; -.
DR HGNC; HGNC:13848; ZNF302.
DR HPA; ENSG00000089335; Tissue enhanced (brain, choroid plexus).
DR neXtProt; NX_Q9NR11; -.
DR OpenTargets; ENSG00000089335; -.
DR PharmGKB; PA37820; -.
DR VEuPathDB; HostDB:ENSG00000089335; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000161431; -.
DR InParanoid; Q9NR11; -.
DR OMA; NHICEKS; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9NR11; -.
DR TreeFam; TF337055; -.
DR PathwayCommons; Q9NR11; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q9NR11; -.
DR BioGRID-ORCS; 55900; 11 hits in 1019 CRISPR screens.
DR ChiTaRS; ZNF302; human.
DR GenomeRNAi; 55900; -.
DR Pharos; Q9NR11; Tdark.
DR PRO; PR:Q9NR11; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9NR11; protein.
DR Bgee; ENSG00000089335; Expressed in endothelial cell and 197 other tissues.
DR ExpressionAtlas; Q9NR11; baseline and differential.
DR Genevisible; Q9NR11; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 7.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..478
FT /note="Zinc finger protein 302"
FT /id="PRO_0000047522"
FT DOMAIN 4..75
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 280..302
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 308..330
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 336..358
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 364..386
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 392..414
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 420..442
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 448..470
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT CROSSLNK 183
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25218447,
FT ECO:0007744|PubMed:28112733"
FT VAR_SEQ 72..169
FT /note="AYPFPLSHSVPASVNFGFSALFEHCSEVTEIFELSELCVFWVLHFLSNSPNS
FT TVEAFSRSKKKKKKKKKRQCFAFLIYFRLGIKMGKQGIINKEGYLY -> DWESRWENK
FT ELSTKKDIYD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:17974005, ECO:0000303|Ref.2,
FT ECO:0000303|Ref.3"
FT /id="VSP_006914"
FT CONFLICT 451
FT /note="N -> H (in Ref. 3; AAF67475)"
FT /evidence="ECO:0000305"
FT CONFLICT 456
FT /note="V -> G (in Ref. 3; AAF67475)"
FT /evidence="ECO:0000305"
FT CONFLICT 464
FT /note="V -> G (in Ref. 3; AAF67475)"
FT /evidence="ECO:0000305"
FT CONFLICT 472
FT /note="E -> G (in Ref. 3; AAF67475)"
FT /evidence="ECO:0000305"
FT CONFLICT 476..478
FT /note="FEV -> LKFRNAGNPSTSLNH (in Ref. 3; AAF67475)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 478 AA; 54814 MW; C7492069228F55A6 CRC64;
MSQVTFSDVA IDFSHEEWAC LDSAQRDLYK DVMVQNYENL VSVGLSVTKP YVIMLLEDGK
EPWMMEKKLS KAYPFPLSHS VPASVNFGFS ALFEHCSEVT EIFELSELCV FWVLHFLSNS
PNSTVEAFSR SKKKKKKKKK RQCFAFLIYF RLGIKMGKQG IINKEGYLYE DSPQPVTMEK
VVKQSYEFSN SNKNLEYTEC DTFRSTFHSK STLSEPQNNS AEGNSHKYDI LKKNLSKKSV
IKSERINGGK KLLNSNKSGA AFNQSKSLTL PQTCNREKIY TCSECGKAFG KQSILSRHWR
IHTGEKPYEC RECGKTFSHG SSLTRHQISH SGEKPYKCIE CGKAFSHGSS LTNHQSTHTG
EKPYECMNCG KSFSRVSLLI QHLRIHTQEK RYECRICGKA FIHSSSLIHH QKSHTGEKPY
ECRECGKAFC CSSHLTQHQR IHSMKKKYEC NKCLKVFSSF SFLVQHQSIH TEEKPFEV
