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CC50A_RAT
ID   CC50A_RAT               Reviewed;         328 AA.
AC   Q6AY41;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Cell cycle control protein 50A {ECO:0000305};
DE   AltName: Full=P4-ATPase flippase complex beta subunit TMEM30A;
DE   AltName: Full=Transmembrane protein 30A;
GN   Name=Tmem30a {ECO:0000312|RGD:1303315}; Synonyms=Cdc50a;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain;
RX   PubMed=24090084; DOI=10.1021/pr400783j;
RA   Parker B.L., Thaysen-Andersen M., Solis N., Scott N.E., Larsen M.R.,
RA   Graham M.E., Packer N.H., Cordwell S.J.;
RT   "Site-specific glycan-peptide analysis for determination of N-glycoproteome
RT   heterogeneity.";
RL   J. Proteome Res. 12:5791-5800(2013).
CC   -!- FUNCTION: Accessory component of a P4-ATPase flippase complex which
CC       catalyzes the hydrolysis of ATP coupled to the transport of
CC       aminophospholipids from the outer to the inner leaflet of various
CC       membranes and ensures the maintenance of asymmetric distribution of
CC       phospholipids. Phospholipid translocation seems also to be implicated
CC       in vesicle formation and in uptake of lipid signaling molecules. The
CC       beta subunit may assist in binding of the phospholipid substrate.
CC       Required for the proper folding, assembly and ER to Golgi exit of the
CC       ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in
CC       regulation of neurite outgrowth, and, reconstituted to liposomes,
CC       predomiminantly transports phosphatidylserine (PS) and to a lesser
CC       extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase
CC       complex seems to play a role in regulation of cell migration probably
CC       involving flippase-mediated translocation of phosphatidylethanolamine
CC       (PE) at the plasma membrane. Required for the formation of the ATP8A2,
CC       ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved
CC       in uptake of platelet-activating factor (PAF). Can also mediate the
CC       export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A,
CC       ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from ER to other membrane
CC       localizations (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of various P4-ATPase flippase complexes which
CC       consists of a catalytic alpha subunit and an accessory beta subunit.
CC       Interacts with ATP8A1 to form a flippase complex; this complex forms an
CC       intermediate phosphoenzyme. Interacts with ATP8A2 to form a flippase
CC       complex (By similarity). TP8B1:TMEM30A and ATP8B2:TMEM30A flippase
CC       complexes have been shown to form intermediate phosphoenzymes in vitro.
CC       Interacts with alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A,
CC       ATP10B, ATP10D, ATP11A, ATP11B and ATP11C.
CC       {ECO:0000250|UniProtKB:Q9NV96}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q9NV96}; Multi-
CC       pass membrane protein {ECO:0000250}. Golgi apparatus
CC       {ECO:0000250|UniProtKB:Q9NV96}. Cytoplasmic vesicle, secretory vesicle
CC       membrane {ECO:0000250|UniProtKB:Q9NV96}. Apical cell membrane
CC       {ECO:0000250|UniProtKB:Q9NV96}. Photoreceptor inner segment
CC       {ECO:0000250|UniProtKB:Q17QL5}. Cell projection, cilium, photoreceptor
CC       outer segment {ECO:0000250|UniProtKB:Q17QL5}.
CC   -!- DOMAIN: The N-terminal domain seems to play a role in the reaction
CC       cycle of the catalytic subunit such as ATP8A2. {ECO:0000250}.
CC   -!- PTM: N-glycosylated. Contains high mannose-type oligosaccharides (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CDC50/LEM3 family. {ECO:0000305}.
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DR   EMBL; BC079203; AAH79203.1; -; mRNA.
DR   RefSeq; NP_001004248.1; NM_001004248.1.
DR   AlphaFoldDB; Q6AY41; -.
DR   SMR; Q6AY41; -.
DR   BioGRID; 256740; 1.
DR   STRING; 10116.ENSRNOP00000015299; -.
DR   GlyGen; Q6AY41; 2 sites.
DR   iPTMnet; Q6AY41; -.
DR   jPOST; Q6AY41; -.
DR   PaxDb; Q6AY41; -.
DR   PRIDE; Q6AY41; -.
DR   Ensembl; ENSRNOT00000015299; ENSRNOP00000015299; ENSRNOG00000010895.
DR   GeneID; 300857; -.
DR   KEGG; rno:300857; -.
DR   UCSC; RGD:1303315; rat.
DR   CTD; 55754; -.
DR   RGD; 1303315; Tmem30a.
DR   eggNOG; KOG2952; Eukaryota.
DR   GeneTree; ENSGT00390000004660; -.
DR   HOGENOM; CLU_025025_1_0_1; -.
DR   InParanoid; Q6AY41; -.
DR   Reactome; R-RNO-6798695; Neutrophil degranulation.
DR   PRO; PR:Q6AY41; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Bgee; ENSRNOG00000010895; Expressed in cerebellum and 20 other tissues.
DR   ExpressionAtlas; Q6AY41; baseline and differential.
DR   Genevisible; Q6AY41; RN.
DR   GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031901; C:early endosome membrane; ISO:RGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031902; C:late endosome membrane; ISO:RGD.
DR   GO; GO:0016020; C:membrane; ISO:RGD.
DR   GO; GO:1990531; C:phospholipid-translocating ATPase complex; ISS:UniProtKB.
DR   GO; GO:0001917; C:photoreceptor inner segment; IEA:UniProtKB-SubCell.
DR   GO; GO:0001750; C:photoreceptor outer segment; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015247; F:aminophospholipid flippase activity; ISO:RGD.
DR   GO; GO:0015917; P:aminophospholipid transport; ISO:RGD.
DR   GO; GO:0045332; P:phospholipid translocation; ISO:RGD.
DR   GO; GO:0010976; P:positive regulation of neuron projection development; ISO:RGD.
DR   GO; GO:0061092; P:positive regulation of phospholipid translocation; ISO:RGD.
DR   GO; GO:0070863; P:positive regulation of protein exit from endoplasmic reticulum; ISO:RGD.
DR   GO; GO:0036010; P:protein localization to endosome; ISO:RGD.
DR   GO; GO:0006855; P:xenobiotic transmembrane transport; ISO:RGD.
DR   InterPro; IPR005045; CDC50/LEM3_fam.
DR   InterPro; IPR030351; TMEM30A.
DR   PANTHER; PTHR10926; PTHR10926; 2.
DR   PANTHER; PTHR10926:SF17; PTHR10926:SF17; 2.
DR   Pfam; PF03381; CDC50; 1.
DR   PIRSF; PIRSF015840; DUF284_TM_euk; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cell membrane; Cell projection; Cytoplasmic vesicle;
KW   Disulfide bond; Glycoprotein; Golgi apparatus; Lipid transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NV96"
FT   CHAIN           2..328
FT                   /note="Cell cycle control protein 50A"
FT                   /id="PRO_0000244472"
FT   TOPO_DOM        2..49
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        71..292
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        314..328
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2..48
FT                   /note="Required for ATPase and aminophospholipid flippase
FT                   activity"
FT                   /evidence="ECO:0000250|UniProtKB:Q17QL5"
FT   REGION          49..315
FT                   /note="Interaction with ATP8A2"
FT                   /evidence="ECO:0000250|UniProtKB:Q17QL5"
FT   REGION          102..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NV96"
FT   CARBOHYD        144
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        261
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0007744|PubMed:24090084"
FT   DISULFID        121..135
FT                   /evidence="ECO:0000250|UniProtKB:Q9NV96"
SQ   SEQUENCE   328 AA;  37173 MW;  EE3B6E0A33FB272F CRC64;
     MAMNYSAKDE VDGGPTGPPG GAAKTRRPDN TAFKQQRLPA WQPILTAGTV LPTFFIIGLI
     FIPIGIGIFV TSNNIREIEG NVFMYYGLSN FYQNHRRYVK SRDDSQLNGD PSALLNPSKE
     CEPYRRNEDK PIAPCGAIAN SMFNDTLELF LVANESDPKP VPILLKKKGI AWWTDKNVKF
     RNPPGKDSLQ EKFKDTTKPV NWHKPVYELD PDDESNNGFI NEDFIVWMRT AALPTFRKLY
     RLIERTDDLH PTLPAGQYYL NITYNYPVHF FDGRKRMILS TISWMGGKNP FLGIAYITIG
     SISFLLGVVL LVINHKYRNS SNTADITI
 
 
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