ZN449_HUMAN
ID ZN449_HUMAN Reviewed; 518 AA.
AC Q6P9G9; Q5JRZ7; Q5JRZ8; Q6NZX2; Q8N3Q1; Q8TED7;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Zinc finger protein 449;
DE AltName: Full=Zinc finger and SCAN domain-containing protein 19;
GN Name=ZNF449; Synonyms=ZSCAN19;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Luo K.T., Yu L.;
RT "Cloning and characterization of a human novel znf gene.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Adipose tissue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Adipose tissue;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q6P9G9; Q96A72: MAGOHB; NbExp=3; IntAct=EBI-10215956, EBI-746778;
CC Q6P9G9; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-10215956, EBI-10288852;
CC Q6P9G9; Q13526: PIN1; NbExp=3; IntAct=EBI-10215956, EBI-714158;
CC Q6P9G9; P57086: SCAND1; NbExp=8; IntAct=EBI-10215956, EBI-745846;
CC Q6P9G9; P10073: ZSCAN22; NbExp=3; IntAct=EBI-10215956, EBI-10178224;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6P9G9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P9G9-2; Sequence=VSP_011949, VSP_011950;
CC Name=3;
CC IsoId=Q6P9G9-3; Sequence=VSP_011951, VSP_011952;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AY280801; AAQ16305.1; -; mRNA.
DR EMBL; AK074194; BAB85013.1; -; mRNA.
DR EMBL; AL833407; CAD38639.1; -; mRNA.
DR EMBL; AL450472; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060768; AAH60768.1; -; mRNA.
DR EMBL; BC065938; AAH65938.1; -; mRNA.
DR CCDS; CCDS14649.1; -. [Q6P9G9-1]
DR RefSeq; NP_689908.3; NM_152695.5. [Q6P9G9-1]
DR RefSeq; XP_011529614.1; XM_011531312.2. [Q6P9G9-1]
DR AlphaFoldDB; Q6P9G9; -.
DR SMR; Q6P9G9; -.
DR BioGRID; 128476; 18.
DR IntAct; Q6P9G9; 6.
DR STRING; 9606.ENSP00000339585; -.
DR iPTMnet; Q6P9G9; -.
DR PhosphoSitePlus; Q6P9G9; -.
DR BioMuta; ZNF449; -.
DR DMDM; 92090580; -.
DR jPOST; Q6P9G9; -.
DR MassIVE; Q6P9G9; -.
DR PaxDb; Q6P9G9; -.
DR PeptideAtlas; Q6P9G9; -.
DR PRIDE; Q6P9G9; -.
DR ProteomicsDB; 67046; -. [Q6P9G9-1]
DR ABCD; Q6P9G9; 3 sequenced antibodies.
DR Antibodypedia; 16515; 187 antibodies from 19 providers.
DR DNASU; 203523; -.
DR Ensembl; ENST00000339249.5; ENSP00000339585.4; ENSG00000173275.13. [Q6P9G9-1]
DR Ensembl; ENST00000370761.7; ENSP00000359797.3; ENSG00000173275.13. [Q6P9G9-2]
DR GeneID; 203523; -.
DR KEGG; hsa:203523; -.
DR MANE-Select; ENST00000339249.5; ENSP00000339585.4; NM_152695.6; NP_689908.3.
DR UCSC; uc004eyr.5; human. [Q6P9G9-1]
DR CTD; 203523; -.
DR GeneCards; ZNF449; -.
DR HGNC; HGNC:21039; ZNF449.
DR HPA; ENSG00000173275; Low tissue specificity.
DR MIM; 300627; gene.
DR neXtProt; NX_Q6P9G9; -.
DR OpenTargets; ENSG00000173275; -.
DR PharmGKB; PA134920699; -.
DR VEuPathDB; HostDB:ENSG00000173275; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000160108; -.
DR HOGENOM; CLU_002678_53_5_1; -.
DR InParanoid; Q6P9G9; -.
DR OMA; GSVFQDY; -.
DR PhylomeDB; Q6P9G9; -.
DR TreeFam; TF336949; -.
DR PathwayCommons; Q6P9G9; -.
DR SignaLink; Q6P9G9; -.
DR BioGRID-ORCS; 203523; 6 hits in 723 CRISPR screens.
DR ChiTaRS; ZNF449; human.
DR GenomeRNAi; 203523; -.
DR Pharos; Q6P9G9; Tdark.
DR PRO; PR:Q6P9G9; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q6P9G9; protein.
DR Bgee; ENSG00000173275; Expressed in oocyte and 171 other tissues.
DR Genevisible; Q6P9G9; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007284; P:spermatogonial cell division; IEA:Ensembl.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 7.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..518
FT /note="Zinc finger protein 449"
FT /id="PRO_0000047597"
FT DOMAIN 30..112
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT ZN_FING 323..345
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 351..373
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 379..401
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 407..429
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 435..457
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 463..485
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 491..513
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 292..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 119..127
FT /note="VDMHDMLLE -> TFAAEYSHY (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_011951"
FT VAR_SEQ 120..164
FT /note="DMHDMLLEELAPVGTAHIPPTMHLESPALQVMGPAQEAPVAEAWI -> RKE
FT CGIFVIGPKEVAEAAGTRPHICLYVIPQPQDFSRVLFLFLFC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_011949"
FT VAR_SEQ 128..518
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_011952"
FT VAR_SEQ 165..518
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_011950"
FT CONFLICT 124
FT /note="M -> I (in Ref. 1; AAQ16305 and 3; CAD38639)"
FT /evidence="ECO:0000305"
FT CONFLICT 187
FT /note="G -> V (in Ref. 1; AAQ16305 and 3; CAD38639)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="P -> L (in Ref. 1; AAQ16305 and 3; CAD38639)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 518 AA; 59932 MW; E6CDA288C489BAB8 CRC64;
MAVALGCAIQ ASLNQGSVFQ EYDTDCEVFR QRFRQFQYRE AAGPHEAFNK LWELCCQWLK
PKMRSKEQIL ELLVLEQFLT ILPTEIETWV REHCPENRER VVSLIEDLQR ELEIPEQQVD
MHDMLLEELA PVGTAHIPPT MHLESPALQV MGPAQEAPVA EAWIPQAGPP ELNYGATGEC
QNFLDPGYPL PKLDMNFSLE NREEPWVKEL QDSKEMKQLL DSKIGFEIGI ENEEDTSKQK
KMETMYPFIV TLEGNALQGP ILQKDYVQLE NQWETPPEDL QTDLAKLVDQ QNPTLGETPE
NSNLEEPLNP KPHKKKSPGE KPHRCPQCGK CFARKSQLTG HQRIHSGEEP HKCPECGKRF
LRSSDLYRHQ RLHTGERPYE CTVCKKRFTR RSHLIGHQRT HSEEETYKCL ECGKSFCHGS
SLKRHLKTHT GEKPHRCHNC GKSFSRLTAL TLHQRTHTEE RPFKCNYCGK SFRQRPSLVI
HLRIHTGEKP YKCTHCSKSF RQRAGLIMHQ VTHFRGLI
