ZN500_HUMAN
ID ZN500_HUMAN Reviewed; 480 AA.
AC O60304; A8K6X7; B4DNN9; Q0VAL2; Q96CQ8; Q9BTG0;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Zinc finger protein 500;
DE AltName: Full=Zinc finger protein with KRAB and SCAN domains 18;
GN Name=ZNF500; Synonyms=KIAA0557, ZKSCAN18;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=9628581; DOI=10.1093/dnares/5.1.31;
RA Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N.,
RA Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. IX. The
RT complete sequences of 100 new cDNA clones from brain which can code for
RT large proteins in vitro.";
RL DNA Res. 5:31-39(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lung, and Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC O60304; O95363: FARS2; NbExp=3; IntAct=EBI-18234077, EBI-2513774;
CC O60304; P14136: GFAP; NbExp=3; IntAct=EBI-18234077, EBI-744302;
CC O60304; Q96MH2: HEXIM2; NbExp=3; IntAct=EBI-18234077, EBI-5460660;
CC O60304; O43464: HTRA2; NbExp=3; IntAct=EBI-18234077, EBI-517086;
CC O60304; P42858: HTT; NbExp=6; IntAct=EBI-18234077, EBI-466029;
CC O60304; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-18234077, EBI-1055254;
CC O60304; O43189: PHF1; NbExp=3; IntAct=EBI-18234077, EBI-530034;
CC O60304; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-18234077, EBI-5235340;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O60304-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O60304-2; Sequence=VSP_055962;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA25483.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB011129; BAA25483.1; ALT_INIT; mRNA.
DR EMBL; AK291792; BAF84481.1; -; mRNA.
DR EMBL; AK297993; BAG60301.1; -; mRNA.
DR EMBL; AC020663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004125; AAH04125.2; -; mRNA.
DR EMBL; BC121013; AAI21014.1; -; mRNA.
DR CCDS; CCDS32383.1; -. [O60304-1]
DR CCDS; CCDS76814.1; -. [O60304-2]
DR RefSeq; NP_001290379.1; NM_001303450.1. [O60304-2]
DR RefSeq; NP_067678.1; NM_021646.3. [O60304-1]
DR AlphaFoldDB; O60304; -.
DR SMR; O60304; -.
DR BioGRID; 117511; 7.
DR IntAct; O60304; 9.
DR STRING; 9606.ENSP00000219478; -.
DR iPTMnet; O60304; -.
DR PhosphoSitePlus; O60304; -.
DR BioMuta; ZNF500; -.
DR MassIVE; O60304; -.
DR PaxDb; O60304; -.
DR PeptideAtlas; O60304; -.
DR PRIDE; O60304; -.
DR ProteomicsDB; 4715; -.
DR ProteomicsDB; 49332; -. [O60304-1]
DR ABCD; O60304; 5 sequenced antibodies.
DR Antibodypedia; 11164; 330 antibodies from 21 providers.
DR DNASU; 26048; -.
DR Ensembl; ENST00000219478.11; ENSP00000219478.5; ENSG00000103199.14. [O60304-1]
DR Ensembl; ENST00000545009.1; ENSP00000445714.1; ENSG00000103199.14. [O60304-2]
DR GeneID; 26048; -.
DR KEGG; hsa:26048; -.
DR MANE-Select; ENST00000219478.11; ENSP00000219478.5; NM_021646.4; NP_067678.1.
DR UCSC; uc002cxp.2; human. [O60304-1]
DR CTD; 26048; -.
DR GeneCards; ZNF500; -.
DR HGNC; HGNC:23716; ZNF500.
DR HPA; ENSG00000103199; Low tissue specificity.
DR neXtProt; NX_O60304; -.
DR OpenTargets; ENSG00000103199; -.
DR PharmGKB; PA134969305; -.
DR VEuPathDB; HostDB:ENSG00000103199; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000154740; -.
DR HOGENOM; CLU_002678_49_3_1; -.
DR InParanoid; O60304; -.
DR OMA; MCPACGR; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; O60304; -.
DR TreeFam; TF341940; -.
DR PathwayCommons; O60304; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; O60304; -.
DR BioGRID-ORCS; 26048; 7 hits in 1099 CRISPR screens.
DR ChiTaRS; ZNF500; human.
DR GenomeRNAi; 26048; -.
DR Pharos; O60304; Tdark.
DR PRO; PR:O60304; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; O60304; protein.
DR Bgee; ENSG00000103199; Expressed in buccal mucosa cell and 167 other tissues.
DR ExpressionAtlas; O60304; baseline and differential.
DR Genevisible; O60304; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR CDD; cd07936; SCAN; 1.
DR Gene3D; 1.10.4020.10; -; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR003309; SCAN_dom.
DR InterPro; IPR038269; SCAN_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF02023; SCAN; 1.
DR Pfam; PF00096; zf-C2H2; 5.
DR SMART; SM00431; SCAN; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS50804; SCAN_BOX; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..480
FT /note="Zinc finger protein 500"
FT /id="PRO_0000047620"
FT DOMAIN 50..134
FT /note="SCAN box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT DOMAIN 224..260
FT /note="KRAB"
FT ZN_FING 325..347
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 353..375
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 381..403
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 409..431
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 437..459
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 161..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 453..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 434..480
FT /note="EKPYTCPACGRGFRRGTDLHKHQRTHMGAGSLPTLQPVAPGGPGAKA -> H
FT ILRSGCT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055962"
SQ SEQUENCE 480 AA; 53674 MW; 9AB0F456F2DC5580 CRC64;
MATVPGLQPL PTLEQDLEQE EILIVKVEED FCLEEEPSVE TEDPSPETFR QLFRLFCYQE
VAGPREALSR LWELCCRWLR PELRTKEQIL ELLVLEQFLT VLPGEIQARV REQQPESGEE
AVVLVEGLQR KPRKHRQRGS ELLSDDEVPL GIGGQFLKHQ AEAQPEDLSL EEEARFSSQQ
PPAQLSHRPQ RGPLLWPERG PPAPRHQEMA SASPFLSAWS QVPVNLEDVA VYLSGEEPRC
MDPAQRDAPL ENEGPGIQLE DGGDGREDAP LRMEWYRVLS ARCQGPGHPL PGQRPAPVRG
LVRPDQPRGG PPPGRRASHG ADKPYTCPEC GKGFSKTSHL TKHQRTHTGE RPYKCLVCGK
GFSDRSNFST HQRVHTGEKP YPCPECGKRF SQSSSLVIHR RTHSGERPYA CTQCGKRFNN
SSHFSAHRRT HTGEKPYTCP ACGRGFRRGT DLHKHQRTHM GAGSLPTLQP VAPGGPGAKA
