ZN527_HUMAN
ID ZN527_HUMAN Reviewed; 609 AA.
AC Q8NB42; B4DVL5;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Zinc finger protein 527;
GN Name=ZNF527; Synonyms=KIAA1829;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8NB42-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NB42-2; Sequence=VSP_040341;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK301135; BAG62727.1; -; mRNA.
DR EMBL; AK091585; BAC03701.1; -; mRNA.
DR EMBL; AC008806; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS42559.1; -. [Q8NB42-1]
DR RefSeq; NP_115829.1; NM_032453.1. [Q8NB42-1]
DR RefSeq; XP_005259386.1; XM_005259329.3. [Q8NB42-2]
DR AlphaFoldDB; Q8NB42; -.
DR SMR; Q8NB42; -.
DR BioGRID; 124104; 1.
DR IntAct; Q8NB42; 1.
DR STRING; 9606.ENSP00000390179; -.
DR iPTMnet; Q8NB42; -.
DR PhosphoSitePlus; Q8NB42; -.
DR BioMuta; ZNF527; -.
DR DMDM; 317373306; -.
DR EPD; Q8NB42; -.
DR jPOST; Q8NB42; -.
DR MassIVE; Q8NB42; -.
DR MaxQB; Q8NB42; -.
DR PaxDb; Q8NB42; -.
DR PeptideAtlas; Q8NB42; -.
DR PRIDE; Q8NB42; -.
DR ProteomicsDB; 72728; -. [Q8NB42-1]
DR ProteomicsDB; 72729; -. [Q8NB42-2]
DR Antibodypedia; 29894; 40 antibodies from 14 providers.
DR DNASU; 84503; -.
DR Ensembl; ENST00000436120.7; ENSP00000390179.2; ENSG00000189164.15. [Q8NB42-1]
DR GeneID; 84503; -.
DR KEGG; hsa:84503; -.
DR MANE-Select; ENST00000436120.7; ENSP00000390179.2; NM_032453.2; NP_115829.1.
DR UCSC; uc010efk.2; human. [Q8NB42-1]
DR CTD; 84503; -.
DR GeneCards; ZNF527; -.
DR HGNC; HGNC:29385; ZNF527.
DR HPA; ENSG00000189164; Low tissue specificity.
DR neXtProt; NX_Q8NB42; -.
DR OpenTargets; ENSG00000189164; -.
DR VEuPathDB; HostDB:ENSG00000189164; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162987; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; Q8NB42; -.
DR OMA; GKRHNEF; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q8NB42; -.
DR PathwayCommons; Q8NB42; -.
DR SignaLink; Q8NB42; -.
DR BioGRID-ORCS; 84503; 10 hits in 1092 CRISPR screens.
DR ChiTaRS; ZNF527; human.
DR GenomeRNAi; 84503; -.
DR Pharos; Q8NB42; Tdark.
DR PRO; PR:Q8NB42; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q8NB42; protein.
DR Bgee; ENSG00000189164; Expressed in buccal mucosa cell and 151 other tissues.
DR ExpressionAtlas; Q8NB42; baseline and differential.
DR Genevisible; Q8NB42; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 9.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 11.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 7.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 11.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 12.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..609
FT /note="Zinc finger protein 527"
FT /id="PRO_0000047639"
FT DOMAIN 14..123
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 274..296
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 302..324
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 330..352
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 358..380
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 386..408
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 414..436
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 442..464
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 470..492
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 498..520
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 526..548
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 554..576
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 582..604
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT VAR_SEQ 54..85
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_040341"
FT CONFLICT 57
FT /note="I -> V (in Ref. 1; BAC03701/BAG62727)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="S -> P (in Ref. 1; BAG62727)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 609 AA; 70902 MW; D1FEBF1EB02D41FB CRC64;
MAVGLCKAMS QGLVTFRDVA LDFSQEEWEW LKPSQKDLYR DVMLENYRNL VWLGLSISKP
NMISLLEQGK EPWMVERKMS QGHCADWESW CEIEELSPKW FIDEDEISQE MVMERLASHG
LECSSFREAW KYKGEFELHQ GNAERHFMQV TAVKEISTGK RDNEFSNSGR SIPLKSVFLT
QQKVPTIQQV HKFDIYDKLF PQNSVIIEYK RLHAEKESLI GNECEEFNQS TYLSKDIGIP
PGEKPYESHD FSKLLSFHSL FTQHQTTHFG KLPHGYDECG DAFSCYSFFT QPQRIHSGEK
PYACNDCGKA FSHDFFLSEH QRTHIGEKPY ECKECNKAFR QSAHLAQHQR IHTGEKPFAC
NECGKAFSRY AFLVEHQRIH TGEKPYECKE CNKAFRQSAH LNQHQRIHTG EKPYECNQCG
KAFSRRIALT LHQRIHTGEK PFKCSECGKT FGYRSHLNQH QRIHTGEKPY ECIKCGKFFR
TDSQLNRHHR IHTGERPFEC SKCGKAFSDA LVLIHHKRSH AGEKPYECNK CGKAFSCGSY
LNQHQRIHTG EKPYECSECG KAFHQILSLR LHQRIHAGEK PYKCNECGNN FSCVSALRRH
QRIHNRETL
