ZN528_HUMAN
ID ZN528_HUMAN Reviewed; 628 AA.
AC Q3MIS6; B3KPN4; Q86T88; Q96JK0;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Zinc finger protein 528;
GN Name=ZNF528; Synonyms=KIAA1827;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Spinal cord;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Heart, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-628 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 8:85-95(2001).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3MIS6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3MIS6-2; Sequence=VSP_023662;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB47456.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK056552; BAG51746.1; -; mRNA.
DR EMBL; AL832630; CAD89949.1; -; mRNA.
DR EMBL; CH471135; EAW72078.1; -; Genomic_DNA.
DR EMBL; BC101712; AAI01713.1; -; mRNA.
DR EMBL; BC111950; AAI11951.1; -; mRNA.
DR EMBL; AB058730; BAB47456.1; ALT_INIT; mRNA.
DR CCDS; CCDS33091.1; -. [Q3MIS6-1]
DR RefSeq; NP_115799.2; NM_032423.2. [Q3MIS6-1]
DR AlphaFoldDB; Q3MIS6; -.
DR SMR; Q3MIS6; -.
DR BioGRID; 124076; 2.
DR STRING; 9606.ENSP00000353652; -.
DR iPTMnet; Q3MIS6; -.
DR PhosphoSitePlus; Q3MIS6; -.
DR BioMuta; ZNF528; -.
DR DMDM; 121942828; -.
DR EPD; Q3MIS6; -.
DR jPOST; Q3MIS6; -.
DR MassIVE; Q3MIS6; -.
DR MaxQB; Q3MIS6; -.
DR PaxDb; Q3MIS6; -.
DR PeptideAtlas; Q3MIS6; -.
DR PRIDE; Q3MIS6; -.
DR ProteomicsDB; 61792; -. [Q3MIS6-1]
DR ProteomicsDB; 61793; -. [Q3MIS6-2]
DR Antibodypedia; 32595; 23 antibodies from 9 providers.
DR DNASU; 84436; -.
DR Ensembl; ENST00000360465.8; ENSP00000353652.3; ENSG00000167555.14. [Q3MIS6-1]
DR GeneID; 84436; -.
DR KEGG; hsa:84436; -.
DR MANE-Select; ENST00000360465.8; ENSP00000353652.3; NM_032423.3; NP_115799.2.
DR UCSC; uc002pzh.3; human. [Q3MIS6-1]
DR CTD; 84436; -.
DR GeneCards; ZNF528; -.
DR HGNC; HGNC:29384; ZNF528.
DR HPA; ENSG00000167555; Low tissue specificity.
DR MIM; 615580; gene.
DR neXtProt; NX_Q3MIS6; -.
DR OpenTargets; ENSG00000167555; -.
DR PharmGKB; PA134869369; -.
DR VEuPathDB; HostDB:ENSG00000167555; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163013; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; Q3MIS6; -.
DR OMA; HNADNPY; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q3MIS6; -.
DR TreeFam; TF341892; -.
DR PathwayCommons; Q3MIS6; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 84436; 6 hits in 1098 CRISPR screens.
DR ChiTaRS; ZNF528; human.
DR GenomeRNAi; 84436; -.
DR Pharos; Q3MIS6; Tdark.
DR PRO; PR:Q3MIS6; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q3MIS6; protein.
DR Bgee; ENSG00000167555; Expressed in buccal mucosa cell and 118 other tissues.
DR ExpressionAtlas; Q3MIS6; baseline and differential.
DR Genevisible; Q3MIS6; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 15.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 15.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 9.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 15.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 15.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..628
FT /note="Zinc finger protein 528"
FT /id="PRO_0000280418"
FT DOMAIN 8..79
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 213..235
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 241..263
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 269..291
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 297..319
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 325..347
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 353..375
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 381..403
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 409..431
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 437..459
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 465..487
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 493..515
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 521..543
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 549..571
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 577..599
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 605..627
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT VAR_SEQ 1..233
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:17974005"
FT /id="VSP_023662"
FT VARIANT 419
FT /note="S -> N (in dbSNP:rs324109)"
FT /id="VAR_052858"
FT CONFLICT 297
FT /note="Y -> H (in Ref. 2; CAD89949)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 628 AA; 72138 MW; BB8704D4A354ED11 CRC64;
MALTQGPLKF MDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLGIC LPDLSVTSML
EQKRDPWTLQ SEEKIANDPD GRECIKGVNT ERSSKLGSNA GNKPCKNQLG FTFQLHLSDL
QLFQAERKIS GCKHFEKPVS DNSSVSPLEK ISSSVKSHLL NKYRNNFDHA PLLPQEQKAH
IREKAYKCNE HGQVFRASAS LTNQVIHNAD NPYKCSECGK VFSCSSKLVI HRRMHTGEKP
YKCHECGKLF SSNSNLSQHQ RIHTGEKPYK CHECDKVFRS SSKLAQHQRI HTGEKPYKCH
ECDKVFNQIA HLVRHQKIHT GEKPYSCNKC GKVFSRHSYL AEHQTVHTGE KPYKCEECGK
AFSVRSSLIT HQLIHTGRKP YKCKECDKVF GRKCFLTSHQ RIHTRERPYG CSQCGKIFSQ
KSDLIRHRKT HTDEKPYKCN KCGTAFREFS DLTAHFLIHS GEKPYECKEC GKVFRYKSSL
TSHHRIHTGE KPYKCNRCGK VFSRSSNLVC HQKIHTGEKP YKCNQCGKVF NQASYLTRHQ
IIHTGERPYR CSKCGKAFRG CSGLTAHLAI HTEKKSHECK ECGKIFTQKS SLTNHHRIHI
GEKPYKCTLC SKVFSHNSDL AQHQRVHS
