ZN543_HUMAN
ID ZN543_HUMAN Reviewed; 600 AA.
AC Q08ER8; Q495U9; Q495V0; Q6ZMP4; Q8NCX4;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 3.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Zinc finger protein 543;
GN Name=ZNF543;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ALA-55; ARG-107;
RP HIS-246 AND VAL-287.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 26-600, AND VARIANTS ALA-55;
RP ARG-107 AND HIS-246.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q08ER8; P60371: KRTAP10-6; NbExp=3; IntAct=EBI-10226133, EBI-12012928;
CC Q08ER8; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-10226133, EBI-10172290;
CC Q08ER8; P23508: MCC; NbExp=3; IntAct=EBI-10226133, EBI-307531;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK131547; BAD18681.1; -; mRNA.
DR EMBL; AC005261; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC101012; AAI01013.1; -; mRNA.
DR EMBL; BC101013; AAI01014.1; -; mRNA.
DR EMBL; BC101014; AAI01015.1; -; mRNA.
DR EMBL; BC101015; AAI01016.1; -; mRNA.
DR EMBL; AL834534; CAD39190.1; -; mRNA.
DR CCDS; CCDS33130.1; -.
DR RefSeq; NP_998763.2; NM_213598.3.
DR AlphaFoldDB; Q08ER8; -.
DR SMR; Q08ER8; -.
DR BioGRID; 125934; 21.
DR IntAct; Q08ER8; 3.
DR STRING; 9606.ENSP00000322545; -.
DR iPTMnet; Q08ER8; -.
DR PhosphoSitePlus; Q08ER8; -.
DR BioMuta; ZNF543; -.
DR DMDM; 313104258; -.
DR jPOST; Q08ER8; -.
DR MassIVE; Q08ER8; -.
DR MaxQB; Q08ER8; -.
DR PaxDb; Q08ER8; -.
DR PeptideAtlas; Q08ER8; -.
DR PRIDE; Q08ER8; -.
DR ProteomicsDB; 58695; -.
DR Antibodypedia; 33243; 115 antibodies from 18 providers.
DR DNASU; 125919; -.
DR Ensembl; ENST00000321545.5; ENSP00000322545.3; ENSG00000178229.8.
DR GeneID; 125919; -.
DR KEGG; hsa:125919; -.
DR MANE-Select; ENST00000321545.5; ENSP00000322545.3; NM_213598.4; NP_998763.2.
DR UCSC; uc002qoi.3; human.
DR CTD; 125919; -.
DR DisGeNET; 125919; -.
DR GeneCards; ZNF543; -.
DR HGNC; HGNC:25281; ZNF543.
DR HPA; ENSG00000178229; Low tissue specificity.
DR MIM; 616847; gene.
DR neXtProt; NX_Q08ER8; -.
DR PharmGKB; PA134914064; -.
DR VEuPathDB; HostDB:ENSG00000178229; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000161437; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; Q08ER8; -.
DR OMA; RIHTQVK; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q08ER8; -.
DR TreeFam; TF341817; -.
DR PathwayCommons; Q08ER8; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q08ER8; -.
DR BioGRID-ORCS; 125919; 13 hits in 1102 CRISPR screens.
DR ChiTaRS; ZNF543; human.
DR GenomeRNAi; 125919; -.
DR Pharos; Q08ER8; Tdark.
DR PRO; PR:Q08ER8; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q08ER8; protein.
DR Bgee; ENSG00000178229; Expressed in cortical plate and 112 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 13.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 13.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 7.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 13.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 13.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..600
FT /note="Zinc finger protein 543"
FT /id="PRO_0000286809"
FT DOMAIN 9..80
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 199..221
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 227..249
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 255..277
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 283..305
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 311..333
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 339..361
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 367..389
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 395..417
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 423..445
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 451..473
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 479..501
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 507..529
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 535..557
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 110..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 55
FT /note="P -> A (in dbSNP:rs6510057)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:17974005"
FT /id="VAR_032171"
FT VARIANT 107
FT /note="Q -> R (in dbSNP:rs8100491)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:17974005"
FT /id="VAR_032172"
FT VARIANT 246
FT /note="L -> H (in dbSNP:rs1968090)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:17974005"
FT /id="VAR_032173"
FT VARIANT 287
FT /note="E -> V (in dbSNP:rs35238720)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_032174"
FT VARIANT 439
FT /note="V -> D (in dbSNP:rs10411486)"
FT /id="VAR_032175"
FT VARIANT 573
FT /note="M -> V (in dbSNP:rs10410649)"
FT /id="VAR_032176"
FT CONFLICT 470
FT /note="F -> L (in Ref. 1; BAD18681)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 600 AA; 68404 MW; EB79C2956B980DED CRC64;
MAASAQVSVT FEDVAVTFTQ EEWGQLDAAQ RTLYQEVMLE TCGLLMSLGC PLFKPELIYQ
LDHRQELWMA TKDLSQSSYP GDNTKPKTTE PTFSHLALPE EVLLQEQLTQ GASKNSQLGQ
SKDQDGPSEM QEVHLKIGIG PQRGKLLEKM SSERDGLGSD DGVCTKITQK QVSTEGDLYE
CDSHGPVTDA LIREEKNSYK CEECGKVFKK NALLVQHERI HTQVKPYECT ECGKTFSKST
HLLQHLIIHT GEKPYKCMEC GKAFNRRSHL TRHQRIHSGE KPYKCSECGK AFTHRSTFVL
HHRSHTGEKP FVCKECGKAF RDRPGFIRHY IIHTGEKPYE CIECGKAFNR RSYLTWHQQI
HTGVKPFECN ECGKAFCESA DLIQHYIIHT GEKPYKCMEC GKAFNRRSHL KQHQRIHTGE
KPYECSECGK AFTHCSTFVL HKRTHTGEKP YECKECGKAF SDRADLIRHF SIHTGEKPYE
CVECGKAFNR SSHLTRHQQI HTGEKPYECI QCGKAFCRSA NLIRHSIIHT GEKPYECSEC
GKAFNRGSSL THHQRIHTGR NPTIVTDVGR PFMTAQTSVN IQELLLGKEF LNITTEENLW
