ZN546_HUMAN
ID ZN546_HUMAN Reviewed; 836 AA.
AC Q86UE3; A8K913;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Zinc finger protein 546;
DE AltName: Full=Zinc finger protein 49;
GN Name=ZNF546; Synonyms=ZNF49;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-201.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-179, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
RN [5]
RP VARIANT [LARGE SCALE ANALYSIS] VAL-15.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK292528; BAF85217.1; -; mRNA.
DR EMBL; CH471126; EAW56930.1; -; Genomic_DNA.
DR EMBL; BC045649; AAH45649.1; -; mRNA.
DR CCDS; CCDS12548.1; -.
DR RefSeq; NP_001284692.1; NM_001297763.1.
DR RefSeq; NP_848639.2; NM_178544.4.
DR RefSeq; XP_011525201.1; XM_011526899.2.
DR AlphaFoldDB; Q86UE3; -.
DR SMR; Q86UE3; -.
DR BioGRID; 130869; 1.
DR IntAct; Q86UE3; 2.
DR STRING; 9606.ENSP00000339823; -.
DR GlyGen; Q86UE3; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q86UE3; -.
DR PhosphoSitePlus; Q86UE3; -.
DR BioMuta; ZNF546; -.
DR DMDM; 229462787; -.
DR jPOST; Q86UE3; -.
DR MassIVE; Q86UE3; -.
DR MaxQB; Q86UE3; -.
DR PaxDb; Q86UE3; -.
DR PeptideAtlas; Q86UE3; -.
DR PRIDE; Q86UE3; -.
DR ProteomicsDB; 69808; -.
DR Antibodypedia; 30449; 53 antibodies from 12 providers.
DR DNASU; 339327; -.
DR Ensembl; ENST00000347077.9; ENSP00000339823.3; ENSG00000187187.14.
DR Ensembl; ENST00000626389.3; ENSP00000487125.1; ENSG00000281526.3.
DR GeneID; 339327; -.
DR KEGG; hsa:339327; -.
DR MANE-Select; ENST00000347077.9; ENSP00000339823.3; NM_178544.5; NP_848639.2.
DR UCSC; uc002oms.3; human.
DR CTD; 339327; -.
DR DisGeNET; 339327; -.
DR GeneCards; ZNF546; -.
DR HGNC; HGNC:28671; ZNF546.
DR HPA; ENSG00000187187; Tissue enhanced (testis).
DR neXtProt; NX_Q86UE3; -.
DR OpenTargets; ENSG00000187187; -.
DR PharmGKB; PA134881362; -.
DR VEuPathDB; HostDB:ENSG00000187187; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163602; -.
DR InParanoid; Q86UE3; -.
DR OMA; IGHQMIH; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q86UE3; -.
DR TreeFam; TF343410; -.
DR PathwayCommons; Q86UE3; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q86UE3; -.
DR BioGRID-ORCS; 339327; 12 hits in 1093 CRISPR screens.
DR ChiTaRS; ZNF546; human.
DR GenomeRNAi; 339327; -.
DR Pharos; Q86UE3; Tdark.
DR PRO; PR:Q86UE3; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q86UE3; protein.
DR Bgee; ENSG00000187187; Expressed in corpus callosum and 99 other tissues.
DR ExpressionAtlas; Q86UE3; baseline and differential.
DR Genevisible; Q86UE3; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 19.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 22.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 12.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 22.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 22.
PE 1: Evidence at protein level;
KW DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc;
KW Zinc-finger.
FT CHAIN 1..836
FT /note="Zinc finger protein 546"
FT /id="PRO_0000234582"
FT DOMAIN 60..131
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 217..239
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 245..267
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 273..295
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 301..323
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 329..351
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 357..379
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 385..407
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 413..435
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 441..463
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 469..491
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 497..519
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 525..547
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 553..575
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 581..603
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 609..631
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 637..659
FT /note="C2H2-type 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 665..687
FT /note="C2H2-type 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 693..715
FT /note="C2H2-type 18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 721..743
FT /note="C2H2-type 19"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 749..771
FT /note="C2H2-type 20"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 777..799
FT /note="C2H2-type 21"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 805..827
FT /note="C2H2-type 22"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT CROSSLNK 179
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VARIANT 15
FT /note="L -> V (in a breast cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035587"
FT VARIANT 201
FT /note="Q -> R (in dbSNP:rs17854378)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_055271"
FT VARIANT 243
FT /note="R -> T (in dbSNP:rs2111543)"
FT /id="VAR_055272"
FT VARIANT 253
FT /note="A -> V (in dbSNP:rs2111544)"
FT /id="VAR_055273"
FT VARIANT 298
FT /note="V -> E (in dbSNP:rs17710336)"
FT /id="VAR_055274"
FT VARIANT 427
FT /note="E -> G (in dbSNP:rs12460371)"
FT /id="VAR_055275"
FT VARIANT 452
FT /note="L -> F (in dbSNP:rs7255186)"
FT /id="VAR_055276"
FT VARIANT 652
FT /note="L -> F (in dbSNP:rs12373540)"
FT /id="VAR_055277"
SQ SEQUENCE 836 AA; 98405 MW; 81AC4B259DDBCB32 CRC64;
MQVDPPLHGP PNDFLIFQII PLHSLSIMPR FLWILCFSME ETQGELTSSC GSKTMANVSL
AFRDVSIDLS QEEWECLDAV QRDLYKDVML ENYSNLVSLG YTIPKPDVIT LLEQEKEPWI
VMREGTRNWF TDLEYKYITK NLLSEKNVCK IYLSQLQTGE KSKNTIHEDT IFRNGLQCKH
EFERQERHQM GCVSQMLIQK QISHPLHPKI HAREKSYECK ECRKAFRQQS YLIQHLRIHT
GERPYKCMEC GKAFCRVGDL RVHHTIHAGE RPYECKECGK AFRLHYHLTE HQRIHSGVKP
YECKECGKAF SRVRDLRVHQ TIHAGERPYE CKECGKAFRL HYQLTEHQRI HTGERPYECK
VCGKTFRVQR HISQHQKIHT GVKPYKCNEC GKAFSHGSYL VQHQKIHTGE KPYECKECGK
SFSFHAELAR HRRIHTGEKP YECRECGKAF RLQTELTRHH RTHTGEKPYE CKECGKAFIC
GYQLTLHLRT HTGEIPYECK ECGKTFSSRY HLTQHYRIHT GEKPYICNEC GKAFRLQGEL
TRHHRIHTCE KPYECKECGK AFIHSNQFIS HQRIHTSEST YICKECGKIF SRRYNLTQHF
KIHTGEKPYI CNECGKAFRF QTELTQHHRI HTGEKPYKCT ECGKAFIRST HLTQHHRIHT
GEKPYECTEC GKTFSRHYHL TQHHRGHTGE KPYICNECGN AFICSYRLTL HQRIHTGELP
YECKECGKTF SRRYHLTQHF RLHTGEKPYS CKECGNAFRL QAELTRHHIV HTGEKPYKCK
ECGKAFSVNS ELTRHHRIHT GEKPYQCKEC GKAFIRSDQL TLHQRNHISE EVLCIM
