ZN566_HUMAN
ID ZN566_HUMAN Reviewed; 418 AA.
AC Q969W8; B7ZL95; Q2M3J1;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Zinc finger protein 566;
GN Name=ZNF566;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-314 AND LYS-328, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q969W8; Q8IYI6: EXOC8; NbExp=3; IntAct=EBI-2555762, EBI-742102;
CC Q969W8; Q9HAQ2: KIF9; NbExp=3; IntAct=EBI-2555762, EBI-8472129;
CC Q969W8; Q96A72: MAGOHB; NbExp=3; IntAct=EBI-2555762, EBI-746778;
CC Q969W8; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-2555762, EBI-5235340;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q969W8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q969W8-2; Sequence=VSP_043419;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK027685; BAB55296.1; -; mRNA.
DR EMBL; AC092295; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC092296; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC007064; AAH07064.1; -; mRNA.
DR EMBL; BC104886; AAI04887.1; -; mRNA.
DR EMBL; BC104888; AAI04889.1; -; mRNA.
DR EMBL; BC143658; AAI43659.1; -; mRNA.
DR CCDS; CCDS12494.1; -. [Q969W8-1]
DR CCDS; CCDS46061.1; -. [Q969W8-2]
DR RefSeq; NP_001138815.1; NM_001145343.1. [Q969W8-2]
DR RefSeq; NP_001138816.1; NM_001145344.1. [Q969W8-1]
DR RefSeq; NP_001138817.1; NM_001145345.1. [Q969W8-1]
DR RefSeq; NP_001287899.1; NM_001300970.1.
DR RefSeq; NP_116227.1; NM_032838.4. [Q969W8-1]
DR RefSeq; XP_006723510.1; XM_006723447.2. [Q969W8-2]
DR AlphaFoldDB; Q969W8; -.
DR SMR; Q969W8; -.
DR BioGRID; 124359; 14.
DR IntAct; Q969W8; 10.
DR STRING; 9606.ENSP00000376010; -.
DR iPTMnet; Q969W8; -.
DR PhosphoSitePlus; Q969W8; -.
DR BioMuta; ZNF566; -.
DR DMDM; 55976754; -.
DR EPD; Q969W8; -.
DR jPOST; Q969W8; -.
DR MassIVE; Q969W8; -.
DR MaxQB; Q969W8; -.
DR PaxDb; Q969W8; -.
DR PeptideAtlas; Q969W8; -.
DR PRIDE; Q969W8; -.
DR ProteomicsDB; 75862; -. [Q969W8-1]
DR ProteomicsDB; 75863; -. [Q969W8-2]
DR Antibodypedia; 16312; 104 antibodies from 15 providers.
DR DNASU; 84924; -.
DR Ensembl; ENST00000392170.7; ENSP00000376010.2; ENSG00000186017.16. [Q969W8-2]
DR Ensembl; ENST00000424129.7; ENSP00000401259.2; ENSG00000186017.16. [Q969W8-1]
DR Ensembl; ENST00000427002.2; ENSP00000400651.2; ENSG00000186017.16. [Q969W8-2]
DR Ensembl; ENST00000434377.6; ENSP00000415520.2; ENSG00000186017.16. [Q969W8-1]
DR Ensembl; ENST00000452939.7; ENSP00000411526.2; ENSG00000186017.16. [Q969W8-1]
DR GeneID; 84924; -.
DR KEGG; hsa:84924; -.
DR MANE-Select; ENST00000452939.7; ENSP00000411526.2; NM_001145344.1; NP_001138816.1.
DR UCSC; uc002oea.4; human. [Q969W8-1]
DR CTD; 84924; -.
DR DisGeNET; 84924; -.
DR GeneCards; ZNF566; -.
DR HGNC; HGNC:25919; ZNF566.
DR HPA; ENSG00000186017; Low tissue specificity.
DR neXtProt; NX_Q969W8; -.
DR OpenTargets; ENSG00000186017; -.
DR PharmGKB; PA134977695; -.
DR VEuPathDB; HostDB:ENSG00000186017; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162125; -.
DR HOGENOM; CLU_002678_44_0_1; -.
DR InParanoid; Q969W8; -.
DR OMA; DCNIQFE; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q969W8; -.
DR TreeFam; TF338743; -.
DR PathwayCommons; Q969W8; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q969W8; -.
DR BioGRID-ORCS; 84924; 18 hits in 1103 CRISPR screens.
DR ChiTaRS; ZNF566; human.
DR GenomeRNAi; 84924; -.
DR Pharos; Q969W8; Tdark.
DR PRO; PR:Q969W8; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q969W8; protein.
DR Bgee; ENSG00000186017; Expressed in cortical plate and 126 other tissues.
DR ExpressionAtlas; Q969W8; baseline and differential.
DR Genevisible; Q969W8; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 4.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 5.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..418
FT /note="Zinc finger protein 566"
FT /id="PRO_0000047656"
FT DOMAIN 6..77
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 169..193
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 199..221
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 227..249
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 255..277
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 283..395
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 311..333
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 339..361
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 367..389
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT CROSSLNK 314
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 328
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 45
FT /note="M -> MA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_043419"
SQ SEQUENCE 418 AA; 49219 MW; 619047F0565141C2 CRC64;
MAQESVMFSD VSVDFSQEEW ECLNDDQRDL YRDVMLENYS NLVSMGHSIS KPNVISYLEQ
GKEPWLADRE LTRGQWPVLE SRCETKKLFL KKEIYEIEST QWEIMEKLTR RDFQCSSFRD
DWECNRQFKK ELGSQGGHFN QLVFTHEDLP TLSHHPSFTL QQIINSKKKF CASKEYRKTF
RHGSQFATHE IIHTIEKPYE CKECGKSFRH PSRLTHHQKI HTGKKPFECK ECGKTFICGS
DLTRHHRIHT GEKPYECKEC GKAFSSGSNF TRHQRIHTGE KPYECKECGK AFSSGSNFTQ
HQRIHTGEKP YECKECGNAF SQSSQLIKHQ RIHTGEKPYE CKECEKAFRS GSDLTRHQRI
HTGEKPYECK ICGKAYSQSS QLISHHRIHT SEKPYEYREC GKNFNYDPQL IQHQNLYW
