ZN628_MOUSE
ID ZN628_MOUSE Reviewed; 1038 AA.
AC Q8CJ78; Q3U2L5; Q6P5B3;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Zinc finger protein 628 {ECO:0000312|MGI:MGI:2665174};
DE AltName: Full=Zinc finger protein expressed in embryonal cells and certain adult organs {ECO:0000303|PubMed:15556296};
GN Name=Znf628 {ECO:0000305};
GN Synonyms=Zec {ECO:0000303|PubMed:15556296},
GN Zfp628 {ECO:0000312|MGI:MGI:2665174};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=ICR; TISSUE=Brain;
RX PubMed=15556296; DOI=10.1016/j.gene.2004.06.016;
RA Chen G.-Y., Muramatsu H., Ichihara-Tanaka K., Muramatsu T.;
RT "ZEC, a zinc finger protein with novel binding specificity and
RT transcription regulatory activity.";
RL Gene 340:71-81(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=NOD; TISSUE=Dendritic cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH TAF4B, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=31932482; DOI=10.1128/mcb.00228-19;
RA Gustafson E.A., Seymour K.A., Sigrist K., Rooij D.G.D.E., Freiman R.N.;
RT "ZFP628 Is a TAF4b-Interacting Transcription Factor Required for Mouse
RT Spermiogenesis.";
RL Mol. Cell. Biol. 40:0-0(2020).
CC -!- FUNCTION: Transcriptional activator (PubMed:15556296, PubMed:31932482).
CC Binds DNA on GT-box consensus sequence 5'-TTGGTT-3' (PubMed:15556296).
CC Plays a role in spermiogenesis (PubMed:31932482).
CC {ECO:0000269|PubMed:15556296, ECO:0000269|PubMed:31932482}.
CC -!- SUBUNIT: Interacts with TAF4B. {ECO:0000269|PubMed:31932482}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15556296}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CJ78-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CJ78-2; Sequence=VSP_019825, VSP_019826;
CC -!- TISSUE SPECIFICITY: Expressed widely in testis, in both germline and
CC somatic cells (PubMed:31932482). Seems to have particularly strong
CC expression in meiotic spermatocytes, postmeiotic round spermatids and
CC Sertoli cells (PubMed:31932482). Not detected in elongating spermatids
CC or mature sperm (at protein level) (PubMed:31932482). Expressed in
CC testis, ovary, spleen, lung, brain, liver and kidney (PubMed:15556296,
CC PubMed:31932482). Expressed in D3 embryonic stem cells and F9 embryonal
CC carcinoma cells (PubMed:15556296). {ECO:0000269|PubMed:15556296,
CC ECO:0000269|PubMed:31932482}.
CC -!- DEVELOPMENTAL STAGE: During development, expression in the brain
CC decreases gradually (PubMed:15556296). Shows increasing expression in
CC testis from 16.5 dpc onwards, with maximum expression at postnatal day
CC 21 (PubMed:31932482). {ECO:0000269|PubMed:15556296,
CC ECO:0000269|PubMed:31932482}.
CC -!- DISRUPTION PHENOTYPE: Viable, with no gross morphological or behavioral
CC phenotypes. Males are infertile with complete absence of mature sperm.
CC Spermiogenesis arrests at the round spermatid stage, accompanied by
CC extensive apoptosis within the seminiferous tubules. Expression of
CC spermiogenesis-associated genes TNP1, TNP2, PRM1 and PRM2 in testis is
CC significantly reduced. {ECO:0000269|PubMed:31932482}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH56945.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH62973.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAN63612.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF435832; AAN63612.1; ALT_INIT; mRNA.
DR EMBL; AK155214; BAE33125.1; -; mRNA.
DR EMBL; BC056945; AAH56945.1; ALT_INIT; mRNA.
DR EMBL; BC062973; AAH62973.1; ALT_INIT; mRNA.
DR CCDS; CCDS51979.1; -. [Q8CJ78-1]
DR RefSeq; NP_739565.2; NM_170759.2. [Q8CJ78-1]
DR RefSeq; XP_006539842.1; XM_006539779.3. [Q8CJ78-1]
DR RefSeq; XP_006539843.1; XM_006539780.3. [Q8CJ78-1]
DR AlphaFoldDB; Q8CJ78; -.
DR SMR; Q8CJ78; -.
DR BioGRID; 231301; 1.
DR STRING; 10090.ENSMUSP00000112058; -.
DR PhosphoSitePlus; Q8CJ78; -.
DR EPD; Q8CJ78; -.
DR PaxDb; Q8CJ78; -.
DR PeptideAtlas; Q8CJ78; -.
DR PRIDE; Q8CJ78; -.
DR ProteomicsDB; 275019; -. [Q8CJ78-1]
DR ProteomicsDB; 275020; -. [Q8CJ78-2]
DR Antibodypedia; 50986; 20 antibodies from 10 providers.
DR DNASU; 232816; -.
DR Ensembl; ENSMUST00000116354; ENSMUSP00000112058; ENSMUSG00000074406. [Q8CJ78-1]
DR GeneID; 232816; -.
DR KEGG; mmu:232816; -.
DR UCSC; uc009eyx.1; mouse. [Q8CJ78-1]
DR CTD; 232816; -.
DR MGI; MGI:2665174; Zfp628.
DR VEuPathDB; HostDB:ENSMUSG00000074406; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00910000144307; -.
DR HOGENOM; CLU_002678_15_0_1; -.
DR InParanoid; Q8CJ78; -.
DR OMA; RPYLCLD; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q8CJ78; -.
DR TreeFam; TF350841; -.
DR BioGRID-ORCS; 232816; 7 hits in 68 CRISPR screens.
DR PRO; PR:Q8CJ78; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8CJ78; protein.
DR Bgee; ENSMUSG00000074406; Expressed in forelimb stylopod and 85 other tissues.
DR Genevisible; Q8CJ78; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 12.
DR SMART; SM00355; ZnF_C2H2; 17.
DR SUPFAM; SSF57667; SSF57667; 9.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 16.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 16.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..1038
FT /note="Zinc finger protein 628"
FT /id="PRO_0000246071"
FT REPEAT 811..821
FT /note="1"
FT /evidence="ECO:0000303|PubMed:15556296"
FT REPEAT 822..832
FT /note="2"
FT /evidence="ECO:0000303|PubMed:15556296"
FT REPEAT 833..843
FT /note="3"
FT /evidence="ECO:0000303|PubMed:15556296"
FT REPEAT 844..854
FT /note="4"
FT /evidence="ECO:0000303|PubMed:15556296"
FT ZN_FING 34..56
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 62..84
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 90..112
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 118..140
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 146..168
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 174..196
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 202..224
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 346..368
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 376..398
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 446..468
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 474..496
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 502..524
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 530..552
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 558..580
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 586..608
FT /note="C2H2-type 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 614..636
FT /note="C2H2-type 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 637..661
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 717..763
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 811..854
FT /note="4 X 11 AA tandem repeats of VQLQP-[AL]-[QT]-[EG]-
FT [VQ]-[ATV]-[ST]"
FT /evidence="ECO:0000303|PubMed:15556296"
FT REGION 922..1038
FT /note="Interaction with TAF4B"
FT /evidence="ECO:0000269|PubMed:31932482"
FT COMPBIAS 256..273
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..652
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 720..738
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 197
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5EBL2"
FT MOD_RES 581
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5EBL2"
FT VAR_SEQ 272..274
FT /note="VVP -> ALL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019825"
FT VAR_SEQ 275..1038
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019826"
SQ SEQUENCE 1038 AA; 109047 MW; D4D8131CDBD0E5CA CRC64;
MAGSHVDMAP ASTTEGTGEK PGPTAPAPTP AAQYECGECG KSFRWSSRLL HHQRTHTGER
PYKCPDCPKA FKGSSALLYH QRGHTGERPY QCPDCPKAFK RSSLLQIHRS VHTGLRAFTC
GQCGLAFKWS SHYQYHLRQH TGERPYPCPD CPKAFKNSSS LRRHRHVHTG ERPYTCGICG
KSFTQSTNLR QHQRVHTGER PFRCPLCPKT FTHSSNLLLH HRTHGPAPGP APAPAPPGET
SRADTKVLVS DAYLQPRSPP EPPAPPPQPP PVVPELFLAA AETTVELVYR CDGCEQGFSS
EELLLEHQPC PGPPVATQSQ DVPAELPQAD SALPQPPPAT PGPPNFACLP CGKSFRTVAG
LSRHQHSHGA ASGQAFRCGS CDGAFPQLAS LLAHQQCHVE EAAAGRPPPQ AEVAEVTCPQ
EPVAPATPAP PPPPPPAPVV SAERPYKCAE CGKAFKGSSG LRYHLRDHTG ERPYQCGECG
KAFKRSSLLA IHQRVHTGLR AFTCGQCGLT FKWSSHYQYH LRLHSGERPY ACTECGKAFR
NTSCLRRHRH VHTGERPHSC SVCGKSFAQT SNLRQHQRVH TGERPFRCPL CPKTFTHSSN
LLLHQRTHSA ERPFACPICG RGFVMAAYLQ RHLRTHTPAT TTSGTTGSAV ASQPPAPLAA
APTPLAAQDV HVLPNLQATL SLEVAGGTAQ PTPPGPAAPS SQTFLLVQTA QGLQLIPSSV
QSPTPPPPPP PPKVILLPPA SAGGPGSGAA RPGPRSVGKA GQGTGVVWFP GPGGLGLQGG
ANAGASGGGQ SLIVLQNVGS GETGPQEVSG VQLQPAQEVA TVQLQPAQEV TTVQLQPAQE
VTTVQLQPLT GQVSNSNGGA GTTEAPNLLL VQSGATEELL TGPGPGEVGD SEAGAGVVQD
VLFETLQTDE GLQSVLVLSG ADGEQTRLCV QEVETLSPGL AEPAATGPSG QKLLIIRSAP
ATDLLENSSV AGGTTTLQLL APSAPGPVSA PVGVPVAPPS QMVQVVPAVA GPGVMAPQNL
PSIQIVQTLP AVQLVHTF
