ZN654_HUMAN
ID ZN654_HUMAN Reviewed; 1128 AA.
AC Q8IZM8; A0A1B0GWA0; Q9H791; Q9NV14;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 4.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Zinc finger protein 654 {ECO:0000305};
DE AltName: Full=Melanoma-associated antigen;
GN Name=ZNF654 {ECO:0000312|HGNC:HGNC:25612};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 553-1128.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 482-1128.
RA Bruno R., d'Orlando O., Altomonte A., Lamaj E., Maio M., Pucillo C.;
RT "Study of the immune profile in metastatic melanoma patients immunized with
RT anti-idiotypic antibody by SEREX analysis.";
RL Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in transcriptional regulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN40504.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA91946.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA91946.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB15007.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC119733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC128650; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK001859; BAA91946.1; ALT_SEQ; mRNA.
DR EMBL; AK024795; BAB15007.1; ALT_INIT; mRNA.
DR EMBL; AF543494; AAN40504.1; ALT_INIT; mRNA.
DR CCDS; CCDS87110.1; -.
DR RefSeq; NP_060763.2; NM_018293.2.
DR AlphaFoldDB; Q8IZM8; -.
DR BioGRID; 120567; 2.
DR STRING; 9606.ENSP00000312141; -.
DR iPTMnet; Q8IZM8; -.
DR PhosphoSitePlus; Q8IZM8; -.
DR BioMuta; ZNF654; -.
DR DMDM; 296453064; -.
DR EPD; Q8IZM8; -.
DR jPOST; Q8IZM8; -.
DR MassIVE; Q8IZM8; -.
DR MaxQB; Q8IZM8; -.
DR PaxDb; Q8IZM8; -.
DR PeptideAtlas; Q8IZM8; -.
DR PRIDE; Q8IZM8; -.
DR ProteomicsDB; 71374; -.
DR Antibodypedia; 32053; 130 antibodies from 23 providers.
DR DNASU; 55279; -.
DR Ensembl; ENST00000636215.2; ENSP00000490842.1; ENSG00000175105.8.
DR MANE-Select; ENST00000636215.2; ENSP00000490842.1; NM_001350134.2; NP_001337063.1.
DR UCSC; uc003dqv.4; human.
DR GeneCards; ZNF654; -.
DR HGNC; HGNC:25612; ZNF654.
DR neXtProt; NX_Q8IZM8; -.
DR OpenTargets; ENSG00000175105; -.
DR PharmGKB; PA134923001; -.
DR VEuPathDB; HostDB:ENSG00000175105; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00950000183034; -.
DR HOGENOM; CLU_026570_1_0_1; -.
DR InParanoid; Q8IZM8; -.
DR OMA; TFKCPAN; -.
DR OrthoDB; 355592at2759; -.
DR PhylomeDB; Q8IZM8; -.
DR TreeFam; TF326007; -.
DR PathwayCommons; Q8IZM8; -.
DR BioGRID-ORCS; 55279; 9 hits in 1077 CRISPR screens.
DR GenomeRNAi; 55279; -.
DR Pharos; Q8IZM8; Tdark.
DR PRO; PR:Q8IZM8; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8IZM8; protein.
DR Genevisible; Q8IZM8; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 1.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1128
FT /note="Zinc finger protein 654"
FT /id="PRO_0000311946"
FT ZN_FING 572..594
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 746..771
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 787..809
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 815..839
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 844..868
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 498..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 891..951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..513
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 891..917
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 918..932
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 933..951
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1123
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9DAU9"
FT MOD_RES 1127
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9DAU9"
FT CONFLICT 534
FT /note="S -> N (in Ref. 3; AAN40504)"
FT /evidence="ECO:0000305"
FT CONFLICT 805
FT /note="H -> Q (in Ref. 2; BAA91946)"
FT /evidence="ECO:0000305"
FT CONFLICT 841
FT /note="I -> T (in Ref. 2; BAA91946 and 3; AAN40504)"
FT /evidence="ECO:0000305"
FT CONFLICT 886..1128
FT /note="Missing (in Ref. 2; BAA91946)"
FT /evidence="ECO:0000305"
FT CONFLICT 925
FT /note="K -> R (in Ref. 2; BAA91946)"
FT /evidence="ECO:0000305"
FT CONFLICT 1035
FT /note="E -> V (in Ref. 2; BAA91946)"
FT /evidence="ECO:0000305"
FT CONFLICT 1095
FT /note="K -> E (in Ref. 2; BAB15007)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1128 AA; 127876 MW; 423D89066E84546A CRC64;
MAEEESDQEA ERLGEELVAI VESPLGPVGL RAAGDGRGGA GSGNCGGGVG ISSRDYCRRF
CQVVEDYAGR WQVPLPQLQV LQTALCCFTT ASASFPDECE HVQYVLSSLA VSFFELLLFF
GRDEFYEEPL KDILGSFQEC QNHLRRYGNV NLELVTRIIR DGGPWEDPVL QAVLKAQPAS
QEIVNKYLSS ENPLFFELRA RYLIACERIP EAMALIKSCI NHPEISKDLY FHQALFTCLF
MSPVEDQLFR EHLLKTDCKS GIDIICNAEK EGKTMLALQL CESFLIPQLQ NGDMYCIWEL
IFIWSKLQLK SNPSKQVFVD QCYQLLRTAT NVRVIFPFMK IIKDEVEEEG LQICVEICGC
ALQLDLHDDP KTKCLIYKTI AHFLPNDLEI LRICALSIFF LERSLEAYRT VEELYKRPDE
EYNEGTSSVQ NRVRFELLPI LKKGLFFDPE FWNFVMIKKN CVALLSDKSA VRFLNESTLE
NNAGNLKRTE EQQGLDEGFD SLTDQSTGET DPDDVSGVQP KGHINTKKNL TALSTSKVDH
NVPRHRCMLC NKEFLGGHIV RHAQAHQKKG SFACVICGRK FRNRGLMQKH LKNHVKKIQR
QQIAAAQQDD QEVTALEEIN CSSSSISFEN GNSDSKDLEV ETLTASSEGN KEVIPEHVAE
FIEIPISVPE DVIENVIENG SPNNSLNNVF KPLTECGDDY EEEEDEEGDY EEDDYDLNQE
TSVIHKINGT VCHPKDIYAT DQEGNFKCPA LGCVRIFKRI GFLNKHAMTV HPTDLNVRQT
VMKWSKGKCK FCQRQFEDSQ HFIDHLNRHS YPNVYFCLHF NCNESFKLPF QLAQHTKSHR
IFQAQCSFPE CHELFEDLPL LYEHEAQHYL SKTPESSAQP SETILWDVQT DSNPNQEKDS
SSNEKQTISL PVSTSKSRKE STEPKTCIES MEKKTDSLVQ NGNERSDDTV SNISLIDQKM
PDIEPNSENN CSSSDIVNGH SEIEQTPLVS SDPALKIDTN RIRTENGSIL PSVVPQEHNT
LPVSQAPSKP NLTSEHTSYG LILTKPYVRP LPPSYLDERY LSMPKRRKFL TDRVDACSDQ
DNVYKKSVKR LRCGKCLTTY CNAEALEAHL AQKKCQTLFG FDSDDESA
