CCAA_CONPI
ID CCAA_CONPI Reviewed; 85 AA.
AC P0C8S5; E2DEK9;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2018, sequence version 2.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=Alpha-conotoxin PrXA {ECO:0000305};
DE Short=Alpha-C-PrXA {ECO:0000303|PubMed:17608455};
DE Flags: Precursor;
OS Conus parius (Cone snail).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Phasmoconus.
OX NCBI_TaxID=505247;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Puillandre N., Olivera B.M.;
RT "Superfamily, scaffold and functions: review and phylogenetic
RT classification of conotoxins.";
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 52-83, PROTEIN SEQUENCE OF 52-83, SYNTHESIS
RP OF 52-83, FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BOND, HYDROXYLATION AT
RP PRO-61; PRO-75 AND PRO-79, AMIDATION AT GLU-83, AND MASS SPECTROMETRY.
RC TISSUE=Venom, and Venom duct;
RX PubMed=17608455; DOI=10.1021/bi700582m;
RA Jimenez E.C., Olivera B.M., Teichert R.W.;
RT "AlphaC-conotoxin PrXA: a new family of nicotinic acetylcholine receptor
RT antagonists.";
RL Biochemistry 46:8717-8724(2007).
CC -!- FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to
CC the nicotinic acetylcholine receptors (nAChR) and thus inhibit them.
CC This toxin inhibits both adult and fetal (alpha-1/beta-1/epsilon/delta
CC and alpha-1/beta-1/gamma/delta subunits) mammalian muscle nicotinic
CC acetylcholine receptors (nAChR). In vivo, it causes paralysis and death
CC in mice and fish. {ECO:0000269|PubMed:17608455}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17608455}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC {ECO:0000269|PubMed:17608455}.
CC -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=3540.7; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17608455};
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DR EMBL; GQ981401; ADN79120.1; -; mRNA.
DR AlphaFoldDB; P0C8S5; -.
DR TCDB; 8.B.36.1.2; the contulakin lt (contulakin lt) family.
DR ConoServer; 2859; PrXA.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Amidation;
KW Direct protein sequencing; Disulfide bond; Hydroxylation;
KW Ion channel impairing toxin; Neurotoxin; Postsynaptic neurotoxin; Secreted;
KW Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..51
FT /evidence="ECO:0000269|PubMed:17608455"
FT /id="PRO_0000444203"
FT PEPTIDE 52..83
FT /note="Alpha-conotoxin PrXA"
FT /evidence="ECO:0000269|PubMed:17608455"
FT /id="PRO_0000366032"
FT MOD_RES 61
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17608455"
FT MOD_RES 75
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17608455"
FT MOD_RES 79
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17608455"
FT MOD_RES 83
FT /note="Glutamic acid 1-amide"
FT /evidence="ECO:0000269|PubMed:17608455"
FT DISULFID 64..71
FT /evidence="ECO:0000269|PubMed:17608455"
SQ SEQUENCE 85 AA; 9492 MW; D4886B945F09A0FB CRC64;
MQTAYWVMVM MMVMWITAPL SEGGKPKLII RGLVPNDLTP QRILRSLISG RTYGIYDAKP
PFSCAGLRGG CVLPPNLRPK FKEGR
