ZNF1_YEAST
ID ZNF1_YEAST Reviewed; 465 AA.
AC P43551; D6VTH8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Respiratory transcription factor ZNF1 {ECO:0000303|PubMed:25673751};
DE AltName: Full=Zinc finger protein 1 {ECO:0000303|PubMed:25673751};
GN Name=ZNF1 {ECO:0000303|PubMed:25673751}; OrderedLocusNames=YFL052W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=11353088; DOI=10.1093/nar/29.10.2181;
RA Akache B., Wu K., Turcotte B.;
RT "Phenotypic analysis of genes encoding yeast zinc cluster proteins.";
RL Nucleic Acids Res. 29:2181-2190(2001).
RN [4]
RP FUNCTION.
RX PubMed=20975739; DOI=10.1038/cr.2010.146;
RA Yang Y., Zhang Z., Li Y., Zhu X.G., Liu Q.;
RT "Identifying cooperative transcription factors by combining ChIP-chip data
RT and knockout data.";
RL Cell Res. 20:1276-1278(2010).
RN [5]
RP INDUCTION, DISRUPTION PHENOTYPE, FUNCTION, AND DNA-BINDING.
RX PubMed=25673751; DOI=10.1093/femsyr/fou002;
RA Tangsombatvichit P., Semkiv M.V., Sibirny A.A., Jensen L.T.,
RA Ratanakhanokchai K., Soontorngun N.;
RT "Zinc cluster protein Znf1, a novel transcription factor of non-
RT fermentative metabolism in Saccharomyces cerevisiae.";
RL FEMS Yeast Res. 15:0-0(2015).
CC -!- FUNCTION: Transcription factor that regulates respiratory growth and
CC plays a critical role in stress adaptation during non-fermentative
CC growth (PubMed:11353088, PubMed:20975739, PubMed:25673751). Binds to
CC promoters of genes involved in non-fermentative metabolism, including
CC processes such as gluconeogenesis (PCK1, FBP1 and MDH2), glyoxylate
CC shunt (MLS1 and ICL1) and the tricarboxylic acid cycle (ACO1)
CC (PubMed:25673751). Plays a role in maintaining mitochondrial morphology
CC and function (PubMed:25673751). Also plays a role in tolerance to pH
CC and osmotic stress, especially during the oxidative metabolism
CC (PubMed:25673751). {ECO:0000269|PubMed:11353088,
CC ECO:0000269|PubMed:20975739, ECO:0000269|PubMed:25673751}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:25673751}.
CC -!- INDUCTION: Expression is increased about 5-fold by ethanol.
CC {ECO:0000269|PubMed:25673751}.
CC -!- DISRUPTION PHENOTYPE: Impairs growth on non-fermentable carbon sources
CC (PubMed:11353088). Results in sensitivity to a combination of low
CC osmolarity and high temperature (PubMed:11353088). Results also in the
CC reduced expression of genes involved in non-fermentable carbon source
CC utilization including MLS1, FBP1, ACO1, PCK1, ICL1, MDH2, SFC1, ADY2,
CC GDH2, FUM1, CIT1 and CIT2, when cells were shifted from glucose to
CC ethanol (PubMed:25673751). Leads to reduced PCK1 and FBP1 enzymatic
CC activities (PubMed:25673751). Leads to defective mitochondrial
CC morphology with unclear structures of the inner membrane cristae when
CC grown in ethanol (PubMed:25673751). {ECO:0000269|PubMed:11353088,
CC ECO:0000269|PubMed:25673751}.
CC -!- SIMILARITY: Belongs to the MAL13 family. {ECO:0000305}.
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DR EMBL; D50617; BAA09189.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12388.1; -; Genomic_DNA.
DR PIR; S56203; S56203.
DR RefSeq; NP_116603.1; NM_001179915.1.
DR AlphaFoldDB; P43551; -.
DR BioGRID; 31095; 139.
DR DIP; DIP-5438N; -.
DR IntAct; P43551; 3.
DR STRING; 4932.YFL052W; -.
DR PaxDb; P43551; -.
DR PRIDE; P43551; -.
DR EnsemblFungi; YFL052W_mRNA; YFL052W; YFL052W.
DR GeneID; 850492; -.
DR KEGG; sce:YFL052W; -.
DR SGD; S000001842; ZNF1.
DR VEuPathDB; FungiDB:YFL052W; -.
DR eggNOG; ENOG502S2FW; Eukaryota.
DR GeneTree; ENSGT00940000176316; -.
DR HOGENOM; CLU_046324_0_0_1; -.
DR InParanoid; P43551; -.
DR OMA; LITERTY; -.
DR BioCyc; YEAST:G3O-30413-MON; -.
DR PRO; PR:P43551; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43551; protein.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:SGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0061410; P:positive regulation of transcription from RNA polymerase II promoter in response to ethanol; IMP:SGD.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR020448; Maltose_ferment_reg_DNA-bd.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR PRINTS; PR00054; FUNGALZNCYS.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..465
FT /note="Respiratory transcription factor ZNF1"
FT /id="PRO_0000114996"
FT DNA_BIND 8..34
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
SQ SEQUENCE 465 AA; 53240 MW; 8F09F46CE290A3C9 CRC64;
MARNRQACDC CCIRRVKCDR KKPCKCCLQH NLQCTYLRPL KKRGPKPVKV RNLKKVDDVQ
VFSKSSSGGI MKVPKALIDQ CLRLYNDKLY VIWPLLCYDD LYELLEKRYD ETCVYWFLVS
LSAATLSDLQ TEIESEGGVT FTGIQLSSFC MSSRQEFDDF NGSDIFKIMT YYCLNRCYAQ
MSNSRTSYRL SCEAVGLIKL AGFHREETLK LLPFDEQQLG RKVYYLLLLT ERYFSVYTHC
ATSLDTTIAP PQPENVTDPR LSLDSFLEMI RVFTVPGKCF FDALATDSAN VTCTEDSLKK
IWRELHTVPL EIEPWSYGYV DISFSRHWIR TLAWKLVLQI SGMRISFLSN SKNTHIPVEI
ARDMLEDTFL IPKNLYAVHG PGISVKALEI ADALVDVVNQ YDQNAESEAW NFLFDISKFV
FSLKHCDSTL VDKFTTKCQC ALITLPLSNP LESTDGSKED VDALP
