CCAP_CONVL
ID CCAP_CONVL Reviewed; 207 AA.
AC E3PQQ8; E3PQQ7;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=ConoCAP;
DE Contains:
DE RecName: Full=ConoCAP-a;
DE AltName: Full=CCAP-vil;
DE Contains:
DE RecName: Full=ConoCAP-b;
DE Contains:
DE RecName: Full=ConoCAP-c;
DE Flags: Precursor;
GN Name=conoCAP;
OS Conus villepinii (Villepin's cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Dauciconus.
OX NCBI_TaxID=257347;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 85-94, SYNTHESIS OF 51-60;
RP 85-94 AND 163-173, FUNCTION, AMIDATION AT GLY-60 AND GLY-173, DISULFIDE
RP BOND, MASS SPECTROMETRY, AND ALANINE-SCANNING MUTAGENESIS.
RC TISSUE=Venom, and Venom duct;
RX PubMed=20923766; DOI=10.1074/jbc.m110.171397;
RA Moeller C., Melaun C., Castillo C., Diaz M.E., Renzelman C.M., Estrada O.,
RA Kuch U., Lokey S., Mari F.;
RT "Functional hypervariability and gene diversity of cardioactive
RT neuropeptides.";
RL J. Biol. Chem. 285:40673-40680(2010).
RN [2]
RP PROTEIN SEQUENCE OF 85-94, SYNTHESIS OF 85-94, FUNCTION, STRUCTURE BY NMR,
RP AMIDATION AT ASN-94, DISULFIDE BOND, AND MUTAGENESIS OF CYS-87 AND CYS-92.
RC TISSUE=Venom;
RX PubMed=20385188; DOI=10.1016/j.peptides.2010.04.002;
RA Miloslavina A., Ebert C., Tietze D., Ohlenschlaeger O., Englert C.,
RA Goerlach M., Imhof D.;
RT "An unusual peptide from Conus villepinii: synthesis, solution structure,
RT and cardioactivity.";
RL Peptides 31:1292-1300(2010).
CC -!- FUNCTION: [ConoCAP-a]: In contrast to other members of the CCAP family
CC which are cardio-accelerators, conoCAP-a decreases the heart frequency
CC in Drosophila larvae (26%), rats and zebrafish embryos. It also reduces
CC the blood pressure in rats. It decreases systolic calcium in
CC ventricular cardiac myocytes, indicating that it may act via impairment
CC of intracellular calcium trafficking.
CC -!- FUNCTION: [ConoCAP-b]: Synthetic conoCAP-b decreases the heart
CC frequency of 23% in Drosophila larvae.
CC -!- FUNCTION: [ConoCAP-c]: Synthetic conoCAP-c decreases the heart
CC frequency of 12% in Drosophila larvae.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC -!- MASS SPECTROMETRY: [ConoCAP-a]: Mass=1148.6; Method=MALDI;
CC Note=Monoisotopic.; Evidence={ECO:0000269|PubMed:20923766};
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DR EMBL; FN868446; CBM40422.1; -; mRNA.
DR EMBL; FN868447; CBM40423.1; -; mRNA.
DR AlphaFoldDB; E3PQQ8; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0043655; C:host extracellular space; TAS:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044554; P:modulation of heart rate in another organism; IDA:UniProtKB.
DR GO; GO:0044555; P:negative regulation of heart rate in another organism; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Amidation; Cardiotoxin; Cleavage on pair of basic residues;
KW Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..48
FT /evidence="ECO:0000255"
FT /id="PRO_0000405955"
FT PEPTIDE 51..60
FT /note="ConoCAP-b"
FT /evidence="ECO:0000305"
FT /id="PRO_0000405956"
FT PROPEP 63..82
FT /evidence="ECO:0000255"
FT /id="PRO_0000405957"
FT PEPTIDE 85..94
FT /note="ConoCAP-a"
FT /evidence="ECO:0000269|PubMed:20385188,
FT ECO:0000269|PubMed:20923766"
FT /id="PRO_0000405958"
FT PROPEP 98..160
FT /evidence="ECO:0000255"
FT /id="PRO_0000405959"
FT PEPTIDE 163..173
FT /note="ConoCAP-c"
FT /evidence="ECO:0000305"
FT /id="PRO_0000405960"
FT PROPEP 177..207
FT /evidence="ECO:0000255"
FT /id="PRO_0000405961"
FT REGION 131..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Glycine amide"
FT /evidence="ECO:0000305|PubMed:20923766"
FT MOD_RES 94
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:20385188"
FT MOD_RES 173
FT /note="Glycine amide"
FT /evidence="ECO:0000305|PubMed:20923766"
FT DISULFID 53..59
FT /evidence="ECO:0000305"
FT DISULFID 87..92
FT DISULFID 165..171
FT /evidence="ECO:0000305"
FT MUTAGEN 85..86
FT /note="Missing: Partial loss of activity of conoCAP-a."
FT MUTAGEN 85
FT /note="P->A: Partial loss of activity of conoCAP-a."
FT MUTAGEN 85
FT /note="Missing: Partial loss of activity of conoCAP-a."
FT MUTAGEN 86
FT /note="F->A: Complete loss of activity of conoCAP-a."
FT MUTAGEN 87
FT /note="C->A: Complete loss of activity of conoCAP-a."
FT /evidence="ECO:0000269|PubMed:20385188"
FT MUTAGEN 88
FT /note="N->A: No change of activity of conoCAP-a."
FT MUTAGEN 89
FT /note="S->A: No change of activity of conoCAP-a."
FT MUTAGEN 90
FT /note="F->A: No change of activity of conoCAP-a."
FT MUTAGEN 91
FT /note="G->A: No change of activity of conoCAP-a."
FT MUTAGEN 92
FT /note="C->A: Complete loss of activity of conoCAP-a."
FT /evidence="ECO:0000269|PubMed:20385188"
FT MUTAGEN 93..94
FT /note="Missing: No change of activity of conoCAP-a."
FT MUTAGEN 93
FT /note="Y->A: No change of activity of conoCAP-a."
FT MUTAGEN 94
FT /note="N->A: No change of activity of conoCAP-a."
FT MUTAGEN 94
FT /note="Missing: No change of activity of conoCAP-a."
FT CONFLICT 164
FT /note="F -> L (in Ref. 1; CBM40422)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 207 AA; 23089 MW; 18E2D2EE1128C484 CRC64;
MVSLGHVLFV ILLPVLLPVA ADDPDDQMLS QISLPSSSRS EYDDNDVSKR VFCNGFTGCG
GRHRDRSRRQ ERYGKRLIPV LAKRPFCNSF GCYNGKRSLS GAGPALSTPV DPSRNNKART
MARMLDAAAS ARHEQQQQLL QQREQRGLES RDPAASGDLS KRLFCNGYGG CRGGKRTLYS
PWLERMNEVA DDRSARNALC TRLGWRE
