CCAP_DROME
ID CCAP_DROME Reviewed; 155 AA.
AC Q9VCW0; Q29QG1;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 17-FEB-2016, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Cardioactive peptide;
DE AltName: Full=Cardioacceleratory peptide 2a;
DE AltName: Full=Crustacean cardioactive peptide;
DE Short=CCAP;
DE Flags: Precursor;
GN Name=CCAP {ECO:0000312|EMBL:AAF56045.2}; ORFNames=CG4910;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF56045.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15242619; DOI=10.1016/j.cub.2004.06.051;
RA Dewey E.M., McNabb S.L., Ewer J., Kuo G.R., Takanishi C.L., Truman J.W.,
RA Honegger H.-W.;
RT "Identification of the gene encoding bursicon, an insect neuropeptide
RT responsible for cuticle sclerotization and wing spreading.";
RL Curr. Biol. 14:1208-1213(2004).
CC -!- FUNCTION: Cardioregulatory neurohormone that increases heart beat rate
CC during adult wing inflation; has no effect on beat amplitude. The
CC effect of CCAP is both ino- and chronotropic (By similarity).
CC {ECO:0000250|UniProtKB:Q8WRC7}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q8WRC7}.
CC -!- TISSUE SPECIFICITY: Central nervous system; most neurons exhibit
CC coexpression with Burs. {ECO:0000269|PubMed:15242619}.
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DR EMBL; AE014297; AAF56045.2; -; Genomic_DNA.
DR EMBL; AE014297; AGB96226.1; -; Genomic_DNA.
DR EMBL; BT024429; ABC86491.1; -; mRNA.
DR RefSeq; NP_001262846.1; NM_001275917.2.
DR RefSeq; NP_651083.2; NM_142826.2.
DR PDB; 1Y49; NMR; -; A=52-60.
DR PDBsum; 1Y49; -.
DR AlphaFoldDB; Q9VCW0; -.
DR SMR; Q9VCW0; -.
DR BioGRID; 67633; 3.
DR IntAct; Q9VCW0; 2.
DR STRING; 7227.FBpp0083726; -.
DR PaxDb; Q9VCW0; -.
DR DNASU; 42683; -.
DR EnsemblMetazoa; FBtr0084333; FBpp0083726; FBgn0039007.
DR EnsemblMetazoa; FBtr0335007; FBpp0307015; FBgn0039007.
DR GeneID; 42683; -.
DR KEGG; dme:Dmel_CG4910; -.
DR CTD; 42683; -.
DR FlyBase; FBgn0039007; CCAP.
DR VEuPathDB; VectorBase:FBgn0039007; -.
DR eggNOG; ENOG502SB93; Eukaryota.
DR HOGENOM; CLU_1697355_0_0_1; -.
DR InParanoid; Q9VCW0; -.
DR OMA; PPFSLFK; -.
DR OrthoDB; 1403075at2759; -.
DR PhylomeDB; Q9VCW0; -.
DR SignaLink; Q9VCW0; -.
DR BioGRID-ORCS; 42683; 0 hits in 1 CRISPR screen.
DR EvolutionaryTrace; Q9VCW0; -.
DR GenomeRNAi; 42683; -.
DR PRO; PR:Q9VCW0; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039007; Expressed in central nervous system and 9 other tissues.
DR ExpressionAtlas; Q9VCW0; baseline and differential.
DR Genevisible; Q9VCW0; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0071855; F:neuropeptide receptor binding; IPI:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:FlyBase.
DR GO; GO:0010460; P:positive regulation of heart rate; IDA:FlyBase.
DR InterPro; IPR024276; CCAP.
DR Pfam; PF11105; CCAP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amidation; Cleavage on pair of basic residues;
KW Disulfide bond; Hormone; Neuropeptide; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..49
FT /evidence="ECO:0000250|UniProtKB:Q8WRC7, ECO:0000255"
FT /id="PRO_0000020851"
FT PEPTIDE 52..60
FT /note="Cardioactive peptide"
FT /evidence="ECO:0000250|UniProtKB:Q8WRC7"
FT /id="PRO_0000020852"
FT PROPEP 64..155
FT /evidence="ECO:0000250|UniProtKB:Q8WRC7"
FT /id="PRO_0000020853"
FT REGION 135..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Cysteine amide"
FT /evidence="ECO:0000250"
FT DISULFID 54..60
FT /evidence="ECO:0000250|UniProtKB:Q8WRC7"
FT STRAND 54..58
FT /evidence="ECO:0007829|PDB:1Y49"
SQ SEQUENCE 155 AA; 18044 MW; E4F44C26D6A39E8A CRC64;
MRTSMRISLR LLALLACAIC SQASLERENN EGTNMANHKL SGVIQWKYEK RPFCNAFTGC
GRKRTYPSYP PFSLFKRNEV EEKPYNNEYL SEGLSDLIDI NAEPAVENVQ KQIMSQAKIF
EAIKEASKEI FRQKNKQKML QNEKEMQQLE ERESK
