CCAP_MANSE
ID CCAP_MANSE Reviewed; 125 AA.
AC Q8WRC7; O76492; P38556;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=Cardioactive peptide;
DE AltName: Full=Cardioacceleratory peptide 2a;
DE AltName: Full=Crustacean cardioactive peptide;
DE Short=CCAP;
DE Flags: Precursor;
GN Name=CCAP {ECO:0000312|EMBL:AAL39064.1};
OS Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC Sphingidae; Sphinginae; Sphingini; Manduca.
OX NCBI_TaxID=7130;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAL39064.1}
RP NUCLEOTIDE SEQUENCE, AND TISSUE SPECIFICITY.
RC TISSUE=Ventral nerve cord {ECO:0000269|PubMed:11683436};
RX PubMed=11683436; DOI=10.1242/jeb.204.16.2803;
RA Loi P.K., Emmal S.A., Park Y., Tublitz N.J.;
RT "Identification, sequence and expression of a crustacean cardioactive
RT peptide (CCAP) gene in the moth Manduca sexta.";
RL J. Exp. Biol. 204:2803-2816(2001).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAC24871.1}
RP NUCLEOTIDE SEQUENCE OF 39-125.
RA Emmal S.A., McGraw H.F., Loi P.K., Huesmann G., Cheung C.C., Tublitz N.J.;
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 45-53, MASS SPECTROMETRY, DISULFIDE BONDS, AND
RP AMIDATION AT CYS-53.
RC TISSUE=Ventral nerve cord {ECO:0000269|PubMed:1426284};
RX PubMed=1426284; DOI=10.1016/0014-5793(92)81436-p;
RA Cheung C.C., Loi P.K., Sylwester A.W., Lee T.D., Tublitz N.J.;
RT "Primary structure of a cardioactive neuropeptide from the tobacco
RT hawkmoth, Manduca sexta.";
RL FEBS Lett. 313:165-168(1992).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC TISSUE=Ventral nerve cord {ECO:0000269|PubMed:3925062};
RX PubMed=3925062; DOI=10.1242/jeb.114.1.365;
RA Tublitz N.J., Truman J.W.;
RT "Insect cardioactive peptides. I. Distribution and molecular
RT characteristics of two cardioacceleratory peptides in the tobacco hawkmoth,
RT Manduca sexta.";
RL J. Exp. Biol. 114:365-379(1985).
RN [5] {ECO:0000305}
RP MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RC TISSUE=Ventral nerve cord {ECO:0000269|PubMed:14706524};
RX PubMed=14706524; DOI=10.1016/j.peptides.2003.07.020;
RA Predel R., Herbert Z., Eckert M.;
RT "Neuropeptides in perisympathetic organs of Manduca sexta: specific
RT composition and changes during the development.";
RL Peptides 24:1457-1464(2003).
CC -!- FUNCTION: Cardioregulatory neurohormone that increases heart beat rate
CC during adult wing inflation; has no effect on beat amplitude. The
CC effect of CCAP is both ino- and chronotropic.
CC {ECO:0000269|PubMed:3925062}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3925062}.
CC -!- TISSUE SPECIFICITY: Abdominal perivisceral organ; major neurohemal
CC release site. Expressed in 116 neurons in post-embryonic central
CC nervous system. Nine pairs of cells are observed in the brain, 4.5
CC pairs in the subesophageal ganglion, three pairs in each thoracic
CC ganglion (T1-T3), three pairs in the first abdominal ganglion (A1),
CC five pairs each in the second to sixth abdominal ganglia (A2-A6) and
CC 7.5 pairs in the terminal ganglion. Expressed in every ganglion in each
CC post-embryonic stage, except in the thoracic ganglia of first- and
CC second-instar larvae. Colocalizes with CAP2b in median neurosecretory
CC cells during the last larval instar through to adults.
CC {ECO:0000269|PubMed:11683436, ECO:0000269|PubMed:14706524,
CC ECO:0000269|PubMed:3925062}.
CC -!- MASS SPECTROMETRY: Mass=956.4; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:1426284};
CC -!- MASS SPECTROMETRY: Mass=956.37; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:14706524};
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DR EMBL; AF451838; AAL39064.1; -; Genomic_DNA.
DR EMBL; AF071800; AAC24871.1; -; mRNA.
DR PIR; S27233; S27233.
DR PDB; 1V46; NMR; -; A=45-53.
DR PDBsum; 1V46; -.
DR AlphaFoldDB; Q8WRC7; -.
DR SMR; Q8WRC7; -.
DR EvolutionaryTrace; Q8WRC7; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005184; F:neuropeptide hormone activity; IDA:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045823; P:positive regulation of heart contraction; IDA:UniProtKB.
DR InterPro; IPR024276; CCAP.
DR Pfam; PF11105; CCAP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amidation; Cleavage on pair of basic residues;
KW Direct protein sequencing; Disulfide bond; Hormone; Neuropeptide; Secreted;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..42
FT /evidence="ECO:0000255, ECO:0000269|PubMed:1426284"
FT /id="PRO_0000020854"
FT PEPTIDE 45..53
FT /note="Cardioactive peptide"
FT /id="PRO_0000020855"
FT PROPEP 57..125
FT /evidence="ECO:0000269|PubMed:1426284"
FT /id="PRO_0000020856"
FT MOD_RES 53
FT /note="Cysteine amide"
FT /evidence="ECO:0000269|PubMed:1426284"
FT DISULFID 47..53
FT /evidence="ECO:0000269|PubMed:1426284"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:1V46"
SQ SEQUENCE 125 AA; 14022 MW; A89C771E01F14714 CRC64;
MTVSRVCLLL LVALVYLDCC YAASIPRNFD PRLSEEIVMA PKKRPFCNAF TGCGRKRSQG
PPGMPAQDLR TKQYLDEEAL GSILDSESAI DELSRQILSE AKLWEAIQEA SAEIARRKQK
EAYIQ
