ZNFX1_CAEEL
ID ZNFX1_CAEEL Reviewed; 2443 AA.
AC E9P860; Q23388;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=NFX1-type zinc finger-containing protein 1 homolog {ECO:0000312|WormBase:ZK1067.2b};
DE EC=3.6.4.13 {ECO:0000305|PubMed:29775580};
GN Name=znfx-1 {ECO:0000303|PubMed:29775580, ECO:0000312|WormBase:ZK1067.2b};
GN ORFNames=ZK1067.2 {ECO:0000312|WormBase:ZK1067.2b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH EGO-1; CSR-1; WAGO-1 AND
RP PRG-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LYS-1067; LEU-1530 AND TYR-1562.
RX PubMed=29775580; DOI=10.1016/j.molcel.2018.04.009;
RA Ishidate T., Ozturk A.R., Durning D.J., Sharma R., Shen E.Z., Chen H.,
RA Seth M., Shirayama M., Mello C.C.;
RT "ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.";
RL Mol. Cell 70:639-649(2018).
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH WAGO-4, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF ARG-271; LEU-290;
RP 407-ARG--LEU-2443; ALA-1047; LYS-1067; HIS-1504; LEU-1530 AND TYR-1562.
RX PubMed=29769721; DOI=10.1038/s41586-018-0132-0;
RA Wan G., Fields B.D., Spracklin G., Shukla A., Phillips C.M., Kennedy S.;
RT "Spatiotemporal regulation of liquid-like condensates in epigenetic
RT inheritance.";
RL Nature 557:679-683(2018).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=32843637; DOI=10.1038/s41467-020-18089-1;
RA Suen K.M., Braukmann F., Butler R., Bensaddek D., Akay A., Lin C.C.,
RA Milonaityte D., Doshi N., Sapetschnig A., Lamond A., Ladbury J.E.,
RA Miska E.A.;
RT "DEPS-1 is required for piRNA-dependent silencing and PIWI condensate
RT organisation in Caenorhabditis elegans.";
RL Nat. Commun. 11:4242-4242(2020).
CC -!- FUNCTION: Epigenetic inheritance factor which, in association with the
CC Argonaute protein wago-4, mediates small RNA-directed transgenerational
CC epigenetic inheritance and thus balances the transgenerational
CC inheritance of epigenetic information (PubMed:29775580,
CC PubMed:29769721). Specifically, maintains a balanced production of
CC small RNAs by preventing the spread of epigenetic signals towards the
CC 5'-end of target mRNAs (PubMed:29775580). Plays a role in small RNA-
CC induced gene silencing in the germline (PubMed:29775580).
CC {ECO:0000269|PubMed:29769721, ECO:0000269|PubMed:29775580}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305|PubMed:29775580};
CC -!- SUBUNIT: Interacts with ego-1, csr-1, wago-1 and prg-1
CC (PubMed:29775580). Interacts with wago-4; the interaction promotes the
CC transmission of epigenetic information across generations
CC (PubMed:29769721). {ECO:0000269|PubMed:29769721,
CC ECO:0000269|PubMed:29775580}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:29769721, ECO:0000269|PubMed:29775580}. Cytoplasm
CC {ECO:0000269|PubMed:29775580}. Cytoplasmic granule
CC {ECO:0000269|PubMed:29769721, ECO:0000269|PubMed:29775580,
CC ECO:0000269|PubMed:32843637}. Note=Co-localizes with wago-4 in P-
CC granules in germline blastomeres until the 100-cell stage
CC (PubMed:29769721). During oocyte maturation, co-localizes with wago-4
CC in liquid-like condensates in the cytoplasm called Z granules
CC (PubMed:29769721). Localizes to perinuclear and cytoplasmic P-granules
CC in germline blastomeres and germ cells (PubMed:29775580,
CC PubMed:29769721). In the adult germline, co-localizes with deps-1 in P-
CC granules (PubMed:32843637). {ECO:0000269|PubMed:29769721,
CC ECO:0000269|PubMed:29775580, ECO:0000269|PubMed:32843637}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:ZK1067.2b};
CC IsoId=E9P860-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:ZK1067.2a};
CC IsoId=E9P860-2; Sequence=VSP_060387;
CC -!- TISSUE SPECIFICITY: Expressed in germs cells (PubMed:29775580,
CC PubMed:29769721). Not expressed in somatic tissues (PubMed:29769721).
CC {ECO:0000269|PubMed:29769721, ECO:0000269|PubMed:29775580}.
CC -!- DEVELOPMENTAL STAGE: Expressed in germ cells at all stages of
CC development. {ECO:0000269|PubMed:29769721,
CC ECO:0000269|PubMed:29775580}.
CC -!- DISRUPTION PHENOTYPE: Defective RNA-induced gene silencing.
CC {ECO:0000269|PubMed:29775580}.
CC -!- SIMILARITY: Belongs to the ZNFX1 family. {ECO:0000305}.
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DR EMBL; BX284602; CAA93884.2; -; Genomic_DNA.
DR EMBL; BX284602; CBZ01828.1; -; Genomic_DNA.
DR RefSeq; NP_001254204.1; NM_001267275.1.
DR RefSeq; NP_001254205.1; NM_001267276.1.
DR AlphaFoldDB; E9P860; -.
DR SMR; E9P860; -.
DR STRING; 6239.ZK1067.2b; -.
DR EPD; E9P860; -.
DR PaxDb; E9P860; -.
DR PeptideAtlas; E9P860; -.
DR EnsemblMetazoa; ZK1067.2a.1; ZK1067.2a.1; WBGene00014208. [E9P860-2]
DR EnsemblMetazoa; ZK1067.2a.2; ZK1067.2a.2; WBGene00014208. [E9P860-2]
DR EnsemblMetazoa; ZK1067.2b.1; ZK1067.2b.1; WBGene00014208. [E9P860-1]
DR UCSC; ZK1067.2; c. elegans.
DR WormBase; ZK1067.2a; CE43256; WBGene00014208; znfx-1. [E9P860-2]
DR WormBase; ZK1067.2b; CE45745; WBGene00014208; znfx-1. [E9P860-1]
DR eggNOG; KOG1807; Eukaryota.
DR GeneTree; ENSGT00940000160694; -.
DR HOGENOM; CLU_233183_0_0_1; -.
DR InParanoid; E9P860; -.
DR OMA; CHPNVEY; -.
DR OrthoDB; 271662at2759; -.
DR PhylomeDB; E9P860; -.
DR PRO; PR:E9P860; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00014208; Expressed in adult organism and 3 other tissues.
DR GO; GO:0031380; C:nuclear RNA-directed RNA polymerase complex; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0031048; P:heterochromatin assembly by small RNA; IBA:GO_Central.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000967; Znf_NFX1.
DR PANTHER; PTHR10887; PTHR10887; 2.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR SMART; SM00438; ZnF_NFX; 4.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Cytoplasm; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Reference proteome; Repeat; RNA-binding;
KW RNA-mediated gene silencing; Zinc; Zinc-finger.
FT CHAIN 1..2443
FT /note="NFX1-type zinc finger-containing protein 1 homolog"
FT /id="PRO_0000448351"
FT DOMAIN 1040..1545
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT ZN_FING 1769..1791
FT /note="NF-X1-type 1"
FT /evidence="ECO:0000255"
FT ZN_FING 1853..1873
FT /note="NF-X1-type 2"
FT /evidence="ECO:0000255"
FT ZN_FING 1912..1930
FT /note="NF-X1-type 3"
FT /evidence="ECO:0000255"
FT ZN_FING 2027..2044
FT /note="NF-X1-type 4"
FT /evidence="ECO:0000255"
FT REGION 212..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 583..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..277
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 278..292
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 321..339
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 545..561
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 583..641
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1061..1068
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT VAR_SEQ 1..231
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060387"
FT MUTAGEN 271
FT /note="R->C: In gg483; disrupted inheritance of RNA-induced
FT gene silencing."
FT /evidence="ECO:0000269|PubMed:29769721"
FT MUTAGEN 290
FT /note="L->F: In gg502; disrupted inheritance of RNA-induced
FT gene silencing."
FT /evidence="ECO:0000269|PubMed:29769721"
FT MUTAGEN 407..2443
FT /note="Missing: In gk458570; disrupted inheritance of RNA-
FT induced gene silencing."
FT /evidence="ECO:0000269|PubMed:29769721"
FT MUTAGEN 1047
FT /note="A->T: In gg511; disrupted inheritance of RNA-induced
FT gene silencing."
FT /evidence="ECO:0000269|PubMed:29769721"
FT MUTAGEN 1067
FT /note="K->A: In ne4382; defective RNA-induced gene
FT silencing."
FT /evidence="ECO:0000269|PubMed:29775580"
FT MUTAGEN 1504
FT /note="H->Y: In gg509; disrupted inheritance of RNA-induced
FT gene silencing."
FT /evidence="ECO:0000269|PubMed:29769721"
FT MUTAGEN 1530
FT /note="L->F: In ne4338 and ne4449; viable and fertile.
FT Defective RNA-induced gene silencing."
FT /evidence="ECO:0000269|PubMed:29775580"
FT MUTAGEN 1562
FT /note="Y->C: In ne4415 and ne4450; viable and fertile.
FT Defective RNA-induced gene silencing at 24 degrees
FT Celsius."
FT /evidence="ECO:0000269|PubMed:29775580"
SQ SEQUENCE 2443 AA; 280315 MW; D7F25167F9E5F413 CRC64;
MSRDKPPQRE VGGRRLPYEM GSLKFDEDPF RGQRRRPWMC FRIGYPTSEF SSEHFMKFVK
RCHTSMIEVG ILLDDEREFL QYQEVREDYL LLKKLIEEGL QKCETIYEKG PLSTPVVFFI
LDQCDEDEVQ NMLDMFRESC YIFHMKRNEI LNWINEMEYE ELESDCSKFA QFIKGVENLT
ESIAMPMEVE TEEFIFGKSS IVSTEDFVED APHKQIAQDQ RETRPIRQPY SMVRNLAPPG
LPPPGISISN SSPPGLSSVS PIPRADTRES RYSTPQPPGL SIPAPPGISL PTPPGISLQN
HQNDQFEQAH STISRMSENS SSSRRRFRDE EVQEEEGDHI LSSEDVHAAR NVCETKILVR
GVPQKNDSEL SKLINGSKLY VRFERPFVKL AHNLELLYVV NYAISSRKSI VRDHSKAIIR
GLFEKDFLLK LKSKFLESSF DNDTWTPEGT ELLFEIFHLF FTTARYKGGF MLTLPRFAPS
WHDFSMAFDI ADMDGLEEAG VGDDELRNLR RLIGYIETWI KRAERERNPS PLALRSYSVY
EKAGSSDSGR QVLSESRGAG SSNVYGHDEV DFVDHRRRDE FGHRDNYRTE NRRHKSPDNF
GEVHRRLDEQ QQQRHEDESS RYRDDSRQSR RADDRRDQYQ YNESTSMENH LGFHNGGGTV
SEHSRDDKFV SPQNCEEKRK YCEEDTDAKP QQPYRFEEIK FEPIWVKCEK SEPPEDFKTL
PSVPVLSEYV NPVEPYLRRI QDDGKYKSVH HYLDVQFRLL KEDLVSPLRD GIDLYKKNGT
CKGRRIEGAP CSDISIFNVE KVDGKQVTER DGYEMRIIWP AQYDILKLLD NDREMKELGL
VMLSCDRFKE DFHLGHIQSS YLMRNGSLHF AVHEETSPFK PNTTYQMAQG TSYLPCYKHV
LENLKRISSF KPLPFERYLV HGSKIIFRPN FQQAEKSEYQ ISEEKKLMKT YNELRSLAAC
ARYTKGKPIP RGVDDDDEDY EFSKSRELSK EDIDLEYRQL QEPIFRPLVG VDIKDSNLIQ
INKKWYNVSR LLDEFHPDYM DESQRLAFCN TFKYELSLIQ GPPGTGKTHI GVQIVKTILQ
NRSYWKITEP ILVVCFTNSG LDNLLERIYQ MIENDEELSK DNGRPKIIRF GSKCDSNYLK
RQNVMRQDVY EQYKSKVSDG AQKKMSKAGA ARRHKADNLA ISSYTLFCSR NKLLSYEMLS
RVMDPNHQME IQQFTYDHVD TKGIPLSPDE AIGCWLLERD FGKATKSQTK KAKKPKFQGA
QLDSEDENKD YFTVEDSDDE EDELDDEKLL DKLFEKMNLE CSGADILSAV HASHADEYYT
KGPWEIVQDK RPSVVVLMEK KTKPCNAKFT VDEQINNLVS EIKDMILSSQ PVPKKDLNDI
KYIFSLARLK RWSLYITWCD ALRSIVTENL PRQIREYREA CENFKNAQNR VDAEIMRMTM
IIGATTTGCS RLRPTLEKVG PRILIVEEAA EVLEAHIISA MISTVEHVVM IGDHKQLRPN
PAVHELGVAY GLRISMFERL VERGLPFSQL RQQHRMNLTI SDKIVKLSFY DNVTDAENVG
LYPDVQGMAT NLFFWSHTSM EESPDEVSWL NKHEISMTVA LVKHLLKQNY TTNDIVVLAT
YSAQKNLMYR EYANVFGSTP DSNVIPVETV DSFQGKERKI VIVSLVRSHR GGRENTGIGF
LAVANRICVA LTRAQHGMYI IGNGAYIMNN SELWNKIVNN LRRSNLIEYN LPLKCVAHGN
IVTVKDPQDF ATKSPEGGCM QKCDTKKFCG HVCERLCHPN MEEEHLQRCL YNCDKKCSNP
QFQHRCKKAC YEECGSCLYL VEVTLDCGHR ITTPCSRINS SKCDQSCTKK LLCGHACAAK
CGEECTLVSE CSQLVGMPLS CGHIKQLTCS KISANEIDLT CDQRCEKTML ACPHKCAEIC
GQPCTVECME VVNVTLGCSH SQDVVCSSFM PGMTDHIECL TKVPKTLSPC KHTELVLCKQ
APSTKLCTRR CTSYLEKCGH TCENDCGICF TTKTHICQNM CQKVLNCGHT CSAKCGESCP
PCKAFCTNKC EHQSCGAGER GFGRDCSKLC ALCVNNCSNK CAHRSCTLKC FEECNVKPCT
EPCTDKLKCG HACLGICGEQ CPKICGTCER NKYIECVSGT SSTSRVHRLI MIPKCYHVFP
VEVLDDHVKK QKEANEKLKC PKCSAFIVGV LRYARYTKKY YLNENMRKLE SNIRNIHQST
LEGRVFQAVQ DSIGEIRNVT TNLTNASEDI LRNFHQKILD IRTSAETFKG KPEHKFKFAS
LLQVANCCLA ITRLLSVSSK FRVSRRKDIP PTFDLMSVRV LGDMPFPKLI DELNRVNIHL
SNTYETFMPG AIIPKLKWLI SRMTVLQQLT SMCHQLVLEK KDIADSDAHA INDACLNMFR
YNEQHNYALN IENFEAIVVK VAPKLLEPTP KFWSWRRLQV PEL
