ZNHI2_HUMAN
ID ZNHI2_HUMAN Reviewed; 403 AA.
AC Q9UHR6; Q3SY14; Q8IUV0;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Zinc finger HIT domain-containing protein 2;
DE AltName: Full=Protein FON;
GN Name=ZNHIT2; Synonyms=C11orf5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=10602999; DOI=10.1007/s003350010016;
RA Lemmens I.H., Farnebo F., Piehl F., Merregaert J., Van de Ven W.J.M.,
RA Larsson C., Kas K.;
RT "Molecular characterization of human and murine c11orf5, a new member of
RT the FAUNA gene cluster.";
RL Mamm. Genome 11:78-80(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP FUNCTION, AND INTERACTION WITH RUVBL2.
RX PubMed=28561026; DOI=10.1038/ncomms15615;
RA Cloutier P., Poitras C., Durand M., Hekmat O., Fiola-Masson E.,
RA Bouchard A., Faubert D., Chabot B., Coulombe B.;
RT "R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2
RT to regulate assembly of U5 small nuclear ribonucleoprotein.";
RL Nat. Commun. 8:15615-15615(2017).
RN [9] {ECO:0007744|PDB:1X4S}
RP STRUCTURE BY NMR OF 1-46.
RX PubMed=17656577; DOI=10.1110/ps.062635107;
RA He F., Umehara T., Tsuda K., Inoue M., Kigawa T., Matsuda T., Yabuki T.,
RA Aoki M., Seki E., Terada T., Shirouzu M., Tanaka A., Sugano S., Muto Y.,
RA Yokoyama S.;
RT "Solution structure of the zinc finger HIT domain in protein FON.";
RL Protein Sci. 16:1577-1587(2007).
RN [10]
RP VARIANT [LARGE SCALE ANALYSIS] PRO-59.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: May act as a bridging factor mediating the interaction
CC between the R2TP/Prefoldin-like (R2TP/PFDL) complex and U5 small
CC nuclear ribonucleoprotein (U5 snRNP) (PubMed:28561026). Required for
CC the interaction of R2TP complex subunit RPAP3 and prefoldin-like
CC subunit URI1 with U5 snRNP proteins EFTUD2 and PRPF8 (PubMed:28561026).
CC May play a role in regulating the composition of the U5 snRNP complex
CC (PubMed:28561026). {ECO:0000269|PubMed:28561026}.
CC -!- SUBUNIT: Interacts (via HIT-type zinc finger) with RUVBL2 in the
CC presence of ATP or ADP; shows a stronger interaction in the presence of
CC ADP. {ECO:0000269|PubMed:28561026}.
CC -!- INTERACTION:
CC Q9UHR6; O95400: CD2BP2; NbExp=4; IntAct=EBI-2557592, EBI-768015;
CC Q9UHR6; Q96N16: JAKMIP1; NbExp=3; IntAct=EBI-2557592, EBI-2680803;
CC Q9UHR6; Q17RB8: LONRF1; NbExp=3; IntAct=EBI-2557592, EBI-2341787;
CC Q9UHR6; Q9Y265: RUVBL1; NbExp=6; IntAct=EBI-2557592, EBI-353675;
CC -!- TISSUE SPECIFICITY: Low expression in most tissues; highly expressed in
CC testis. {ECO:0000269|PubMed:10602999}.
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DR EMBL; AF119497; AAF23591.1; -; mRNA.
DR EMBL; BC038089; AAH38089.1; -; mRNA.
DR EMBL; BC052240; AAH52240.1; -; mRNA.
DR CCDS; CCDS8094.1; -.
DR RefSeq; NP_055020.1; NM_014205.3.
DR PDB; 1X4S; NMR; -; A=1-46.
DR PDBsum; 1X4S; -.
DR AlphaFoldDB; Q9UHR6; -.
DR SMR; Q9UHR6; -.
DR BioGRID; 107200; 128.
DR IntAct; Q9UHR6; 27.
DR STRING; 9606.ENSP00000308548; -.
DR iPTMnet; Q9UHR6; -.
DR PhosphoSitePlus; Q9UHR6; -.
DR BioMuta; ZNHIT2; -.
DR DMDM; 27734232; -.
DR EPD; Q9UHR6; -.
DR jPOST; Q9UHR6; -.
DR MassIVE; Q9UHR6; -.
DR MaxQB; Q9UHR6; -.
DR PaxDb; Q9UHR6; -.
DR PeptideAtlas; Q9UHR6; -.
DR PRIDE; Q9UHR6; -.
DR ProteomicsDB; 84405; -.
DR Antibodypedia; 29668; 69 antibodies from 21 providers.
DR DNASU; 741; -.
DR Ensembl; ENST00000310597.6; ENSP00000308548.4; ENSG00000174276.7.
DR GeneID; 741; -.
DR KEGG; hsa:741; -.
DR MANE-Select; ENST00000310597.6; ENSP00000308548.4; NM_014205.4; NP_055020.1.
DR UCSC; uc001ocw.4; human.
DR CTD; 741; -.
DR DisGeNET; 741; -.
DR GeneCards; ZNHIT2; -.
DR HGNC; HGNC:1177; ZNHIT2.
DR HPA; ENSG00000174276; Tissue enhanced (testis).
DR MIM; 604575; gene.
DR neXtProt; NX_Q9UHR6; -.
DR OpenTargets; ENSG00000174276; -.
DR PharmGKB; PA25496; -.
DR VEuPathDB; HostDB:ENSG00000174276; -.
DR eggNOG; KOG4317; Eukaryota.
DR GeneTree; ENSGT00390000017147; -.
DR HOGENOM; CLU_039057_1_0_1; -.
DR InParanoid; Q9UHR6; -.
DR OMA; VPYCSLR; -.
DR OrthoDB; 1599898at2759; -.
DR PhylomeDB; Q9UHR6; -.
DR TreeFam; TF324864; -.
DR PathwayCommons; Q9UHR6; -.
DR SignaLink; Q9UHR6; -.
DR BioGRID-ORCS; 741; 701 hits in 1085 CRISPR screens.
DR EvolutionaryTrace; Q9UHR6; -.
DR GenomeRNAi; 741; -.
DR Pharos; Q9UHR6; Tbio.
DR PRO; PR:Q9UHR6; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9UHR6; protein.
DR Bgee; ENSG00000174276; Expressed in right testis and 95 other tissues.
DR ExpressionAtlas; Q9UHR6; baseline and differential.
DR Genevisible; Q9UHR6; HS.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR007529; Znf_HIT.
DR InterPro; IPR039646; ZNHIT2.
DR PANTHER; PTHR15555; PTHR15555; 1.
DR Pfam; PF04438; zf-HIT; 1.
DR PROSITE; PS51083; ZF_HIT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Metal-binding; Phosphoprotein;
KW Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..403
FT /note="Zinc finger HIT domain-containing protein 2"
FT /id="PRO_0000173548"
FT ZN_FING 7..41
FT /note="HIT-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT REGION 72..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 7
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 10
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 22
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 25
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 38
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 41
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 161
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18691976,
FT ECO:0007744|PubMed:23186163"
FT VARIANT 59
FT /note="A -> P (in a breast cancer sample; somatic mutation;
FT dbSNP:rs1185334988)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035720"
FT VARIANT 155
FT /note="E -> K (in dbSNP:rs11556920)"
FT /id="VAR_053757"
FT VARIANT 176
FT /note="A -> V (in dbSNP:rs35983251)"
FT /id="VAR_053758"
FT STRAND 5..7
FT /evidence="ECO:0007829|PDB:1X4S"
FT STRAND 16..18
FT /evidence="ECO:0007829|PDB:1X4S"
FT TURN 23..25
FT /evidence="ECO:0007829|PDB:1X4S"
FT STRAND 28..31
FT /evidence="ECO:0007829|PDB:1X4S"
FT HELIX 32..38
FT /evidence="ECO:0007829|PDB:1X4S"
FT HELIX 41..44
FT /evidence="ECO:0007829|PDB:1X4S"
SQ SEQUENCE 403 AA; 42884 MW; 2384BF806CC40E71 CRC64;
MEPAGPCGFC PAGEVQPARY TCPRCNAPYC SLRCYRTHGT CAENFYRDQV LGELRGCSAP
PSRLASALRR LRQQRETEDE PGEAGLSSGP APGGLSGLWE RLAPGEKAAF ERLLSRGEAG
RLLPPWRPWW WNRGAGPQLL EELDNAPGSD AAELELAPAR TPPDSVKDAS AAEPAAAERV
LGDVPGACTP VVPTRIPAIV SLSRGPVSPL VRFQLPNVLF AYAHTLALYH GGDDALLSDF
CATLLGVSGA LGAQQVFASA EEALQAAAHV LEAGEHPPGP LGTRGAMHEV ARILLGEGPT
NQKGYTLAAL GDLAQTLGRA RKQAVAREER DHLYRARKKC QFLLAWTNEN EAALTPLALD
CARAHQAHAV VAEEVAALTG ELERLWGGPV PPAPRTLIEE LPS
