ZNRD2_HUMAN
ID ZNRD2_HUMAN Reviewed; 199 AA.
AC O60232;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Protein ZNRD2 {ECO:0000305};
DE AltName: Full=Autoantigen p27 {ECO:0000303|PubMed:9486406};
DE AltName: Full=Sjoegren syndrome/scleroderma autoantigen 1;
DE AltName: Full=Zinc ribbon domain-containing protein 2 {ECO:0000305};
GN Name=ZNRD2 {ECO:0000312|HGNC:HGNC:11328};
GN Synonyms=SSSCA1 {ECO:0000312|HGNC:HGNC:11328};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=9486406; DOI=10.1046/j.1365-2249.1998.00517.x;
RA Muro Y., Yamada T., Himeno M., Sugimoto K.;
RT "cDNA cloning of a novel autoantigen targeted by a minor subset of anti-
RT centromere antibodies.";
RL Clin. Exp. Immunol. 111:372-376(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic kidney;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E.,
RA Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y.,
RA Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Might play a role in mitosis. Antigenic molecule. Could be a
CC centromere-associated protein. May induce anti-centromere antibodies.
CC {ECO:0000305|PubMed:9486406}.
CC -!- INTERACTION:
CC O60232; P35609: ACTN2; NbExp=3; IntAct=EBI-741415, EBI-77797;
CC O60232; P51946: CCNH; NbExp=4; IntAct=EBI-741415, EBI-741406;
CC O60232; P78371: CCT2; NbExp=6; IntAct=EBI-741415, EBI-357407;
CC O60232; P49368: CCT3; NbExp=6; IntAct=EBI-741415, EBI-356673;
CC O60232; P48643: CCT5; NbExp=4; IntAct=EBI-741415, EBI-355710;
CC O60232; Q99832: CCT7; NbExp=7; IntAct=EBI-741415, EBI-357046;
CC O60232; V9HW96: HEL-S-100n; NbExp=3; IntAct=EBI-741415, EBI-10330207;
CC O60232; V9HW37: HEL-S-69; NbExp=3; IntAct=EBI-741415, EBI-10186233;
CC O60232; Q0VD86: INCA1; NbExp=3; IntAct=EBI-741415, EBI-6509505;
CC O60232; O75928-2: PIAS2; NbExp=3; IntAct=EBI-741415, EBI-348567;
CC O60232; P78317: RNF4; NbExp=3; IntAct=EBI-741415, EBI-2340927;
CC O60232; O75971: SNAPC5; NbExp=3; IntAct=EBI-741415, EBI-749483;
CC O60232; G2XKQ0: SUMO1P1; NbExp=3; IntAct=EBI-741415, EBI-10175576;
CC O60232; Q9Y228: TRAF3IP3; NbExp=3; IntAct=EBI-741415, EBI-765817;
CC O60232; Q7KZS0: UBE2I; NbExp=3; IntAct=EBI-741415, EBI-10180829;
CC O60232; Q9UJ78-2: ZMYM5; NbExp=3; IntAct=EBI-741415, EBI-17634549;
CC O60232; Q9Q2G4: ORF; Xeno; NbExp=4; IntAct=EBI-741415, EBI-6248094;
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DR EMBL; AB001740; BAA25263.1; -; mRNA.
DR EMBL; BC014791; AAH14791.1; -; mRNA.
DR CCDS; CCDS8104.1; -.
DR RefSeq; NP_006387.1; NM_006396.2.
DR PDB; 6HCZ; X-ray; 2.30 A; A/B=1-199.
DR PDBsum; 6HCZ; -.
DR AlphaFoldDB; O60232; -.
DR SMR; O60232; -.
DR BioGRID; 115788; 299.
DR IntAct; O60232; 110.
DR STRING; 9606.ENSP00000312318; -.
DR ChEMBL; CHEMBL4105996; -.
DR GlyGen; O60232; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; O60232; -.
DR PhosphoSitePlus; O60232; -.
DR BioMuta; SSSCA1; -.
DR EPD; O60232; -.
DR jPOST; O60232; -.
DR MassIVE; O60232; -.
DR MaxQB; O60232; -.
DR PaxDb; O60232; -.
DR PeptideAtlas; O60232; -.
DR PRIDE; O60232; -.
DR ProteomicsDB; 49259; -.
DR Antibodypedia; 29847; 181 antibodies from 25 providers.
DR DNASU; 10534; -.
DR Ensembl; ENST00000309328.8; ENSP00000312318.3; ENSG00000173465.8.
DR GeneID; 10534; -.
DR KEGG; hsa:10534; -.
DR MANE-Select; ENST00000309328.8; ENSP00000312318.3; NM_006396.3; NP_006387.1.
DR UCSC; uc001oek.4; human.
DR CTD; 10534; -.
DR DisGeNET; 10534; -.
DR GeneCards; ZNRD2; -.
DR HGNC; HGNC:11328; ZNRD2.
DR HPA; ENSG00000173465; Low tissue specificity.
DR MIM; 606044; gene.
DR neXtProt; NX_O60232; -.
DR OpenTargets; ENSG00000173465; -.
DR PharmGKB; PA36152; -.
DR VEuPathDB; HostDB:ENSG00000173465; -.
DR eggNOG; KOG4537; Eukaryota.
DR GeneTree; ENSGT00390000013169; -.
DR HOGENOM; CLU_058702_0_0_1; -.
DR InParanoid; O60232; -.
DR OMA; VACQELN; -.
DR OrthoDB; 1602560at2759; -.
DR PhylomeDB; O60232; -.
DR TreeFam; TF320182; -.
DR PathwayCommons; O60232; -.
DR SignaLink; O60232; -.
DR BioGRID-ORCS; 10534; 57 hits in 1080 CRISPR screens.
DR ChiTaRS; SSSCA1; human.
DR GenomeRNAi; 10534; -.
DR Pharos; O60232; Tdark.
DR PRO; PR:O60232; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; O60232; protein.
DR Bgee; ENSG00000173465; Expressed in body of pancreas and 113 other tissues.
DR ExpressionAtlas; O60232; baseline and differential.
DR Genevisible; O60232; HS.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000278; P:mitotic cell cycle; TAS:ProtInc.
DR InterPro; IPR009563; SSSCA1.
DR Pfam; PF06677; Auto_anti-p27; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division; Mitosis;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT CHAIN 2..199
FT /note="Protein ZNRD2"
FT /id="PRO_0000072201"
FT REGION 100..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT MOD_RES 94
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17525332"
FT VARIANT 21
FT /note="T -> M (in dbSNP:rs35971725)"
FT /id="VAR_051383"
FT HELIX 18..43
FT /evidence="ECO:0007829|PDB:6HCZ"
FT STRAND 47..52
FT /evidence="ECO:0007829|PDB:6HCZ"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:6HCZ"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:6HCZ"
FT TURN 71..75
FT /evidence="ECO:0007829|PDB:6HCZ"
SQ SEQUENCE 199 AA; 21474 MW; EF08439FDFFA1DAB CRC64;
MALNGAEVDD FSWEPPTEAE TKVLQARRER QDRISRLMGD YLLRGYRMLG ETCADCGTIL
LQDKQRKIYC VACQELDSDV DKDNPALNAQ AALSQAREHQ LASASELPLG SRPAPQPPVP
RPEHCEGAAA GLKAAQGPPA PAVPPNTDVM ACTQTALLQK LTWASAELGS STSLETSIQL
CGLIRACAEA LRSLQQLQH
