ZNT1_MOUSE
ID ZNT1_MOUSE Reviewed; 503 AA.
AC Q60738;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Zinc transporter 1;
DE Short=ZnT-1;
DE AltName: Full=Solute carrier family 30 member 1;
GN Name=Slc30a1; Synonyms=Znt1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Brain;
RX PubMed=7882967; DOI=10.1002/j.1460-2075.1995.tb07042.x;
RA Palmiter R.D., Findley S.D.;
RT "Cloning and functional characterization of a mammalian zinc transporter
RT that confers resistance to zinc.";
RL EMBO J. 14:639-649(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in zinc transport out of the cell. Lethality
CC of knockout early in gestation suggests a role of the protein in fetal
CC zinc acquisition and retention.
CC -!- SUBUNIT: Multimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely expressed.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR EMBL; U17132; AAA79233.1; -; Genomic_DNA.
DR EMBL; BC052166; AAH52166.1; -; mRNA.
DR CCDS; CCDS15625.1; -.
DR PIR; S54302; S54302.
DR RefSeq; NP_033605.1; NM_009579.3.
DR AlphaFoldDB; Q60738; -.
DR SMR; Q60738; -.
DR BioGRID; 204704; 2.
DR STRING; 10090.ENSMUSP00000042410; -.
DR GlyGen; Q60738; 1 site.
DR iPTMnet; Q60738; -.
DR PhosphoSitePlus; Q60738; -.
DR SwissPalm; Q60738; -.
DR EPD; Q60738; -.
DR jPOST; Q60738; -.
DR MaxQB; Q60738; -.
DR PaxDb; Q60738; -.
DR PRIDE; Q60738; -.
DR ProteomicsDB; 275308; -.
DR Antibodypedia; 20709; 234 antibodies from 26 providers.
DR DNASU; 22782; -.
DR Ensembl; ENSMUST00000044954; ENSMUSP00000042410; ENSMUSG00000037434.
DR GeneID; 22782; -.
DR KEGG; mmu:22782; -.
DR UCSC; uc007edc.1; mouse.
DR CTD; 7779; -.
DR MGI; MGI:1345281; Slc30a1.
DR VEuPathDB; HostDB:ENSMUSG00000037434; -.
DR eggNOG; KOG1483; Eukaryota.
DR GeneTree; ENSGT00940000156484; -.
DR HOGENOM; CLU_013430_4_3_1; -.
DR InParanoid; Q60738; -.
DR OMA; HADMGMN; -.
DR OrthoDB; 820567at2759; -.
DR PhylomeDB; Q60738; -.
DR TreeFam; TF313924; -.
DR Reactome; R-MMU-435368; Zinc efflux and compartmentalization by the SLC30 family.
DR BioGRID-ORCS; 22782; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q60738; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q60738; protein.
DR Bgee; ENSMUSG00000037434; Expressed in ectoplacental cone and 89 other tissues.
DR ExpressionAtlas; Q60738; baseline and differential.
DR Genevisible; Q60738; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; ISO:MGI.
DR GO; GO:0030315; C:T-tubule; ISO:MGI.
DR GO; GO:0019855; F:calcium channel inhibitor activity; IDA:BHF-UCL.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; ISO:MGI.
DR GO; GO:0070574; P:cadmium ion transmembrane transport; ISO:MGI.
DR GO; GO:0070509; P:calcium ion import; ISO:MGI.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IDA:BHF-UCL.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IDA:BHF-UCL.
DR GO; GO:0071585; P:detoxification of cadmium ion; ISO:MGI.
DR GO; GO:0010312; P:detoxification of zinc ion; IBA:GO_Central.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0090281; P:negative regulation of calcium ion import; IDA:BHF-UCL.
DR GO; GO:0046929; P:negative regulation of neurotransmitter secretion; ISO:MGI.
DR GO; GO:0071584; P:negative regulation of zinc ion transmembrane import; IDA:BHF-UCL.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IDA:BHF-UCL.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport;
KW Zinc; Zinc transport.
FT CHAIN 1..503
FT /note="Zinc transporter 1"
FT /id="PRO_0000206092"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..35
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..113
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..303
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..503
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 140..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..156
FT /note="6 X 2 AA approximate repeats of H-G"
FT COMPBIAS 183..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 502
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 503 AA; 54716 MW; 7C4FF93FC13CDA22 CRC64;
MGCWGRNRGR LLCMLLLTFM FMVLEVVVSR VTASLAMLSD SFHMLSDVLA LVVALVAERF
ARRTHATQKN TFGWIRAEVM GALVNAIFLT GLCFAILLEA VERFIEPHEM QQPLVVLSVG
VAGLLVNVLG LCLFHHHSGE GQGAGHGHSH GHGHGHLAKG ARKAGRAGVE AGAPPGRAPD
QEETNTLVAN TSNSNGLKAD QAEPEKLRSD DPVDVQVNGN LIQESDNLEA EDNRAGQLNM
RGVFLHVLGD ALGSVIVVVN ALVFYFNWKG CTEDDFCTNP CFPDPCKSSV EIINSTQAPM
RDAGPCWVLY LDPTLCIIMV CILLYTTYPL LKESALILLQ TVPKQIDIKH LVKELRDVDG
VEEVHELHVW QLAGSRIIAT AHIKCEDPAS YMQVAKTIKD VFHNHGIHAT TIQPEFASVG
SKSSVLPCEL ACRTQCALKQ CCGTRPQVHS GKDAEKAPTV SISCLELSEN LEKKARRTKA
EGSLPAVVIE IKNVPNKQPE SSL
