ZNT8_HUMAN
ID ZNT8_HUMAN Reviewed; 369 AA.
AC Q8IWU4; A0AVP9; A5YM39; B4DPE0; Q8TCL3;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Zinc transporter 8;
DE Short=ZnT-8;
DE AltName: Full=Solute carrier family 30 member 8;
GN Name=SLC30A8; Synonyms=ZNT8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Pancreas;
RX PubMed=15331542; DOI=10.2337/diabetes.53.9.2330;
RA Chimienti F., Devergnas S., Favier A., Seve M.;
RT "Identification and cloning of a beta-cell-specific zinc transporter, ZnT-
RT 8, localized into insulin secretory granules.";
RL Diabetes 53:2330-2337(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Amygdala, and Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=16158222; DOI=10.1007/s10534-005-3687-9;
RA Chimienti F., Favier A., Seve M.;
RT "ZnT-8, a pancreatic beta-cell-specific zinc transporter.";
RL BioMetals 18:313-317(2005).
RN [8]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=16984975; DOI=10.1242/jcs.03164;
RA Chimienti F., Devergnas S., Pattou F., Schuit F., Garcia-Cuenca R.,
RA Vandewalle B., Kerr-Conte J., Van Lommel L., Grunwald D., Favier A.,
RA Seve M.;
RT "In vivo expression and functional characterization of the zinc transporter
RT ZnT8 in glucose-induced insulin secretion.";
RL J. Cell Sci. 119:4199-4206(2006).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=17118530; DOI=10.1016/j.mce.2006.10.010;
RA Smidt K., Pedersen S.B., Brock B., Schmitz O., Fisker S., Bendix J.,
RA Wogensen L., Rungby J.;
RT "Zinc-transporter genes in human visceral and subcutaneous adipocytes: lean
RT versus obese.";
RL Mol. Cell. Endocrinol. 264:68-73(2007).
RN [10]
RP TISSUE SPECIFICITY.
RX PubMed=17971500; DOI=10.1189/jlb.0307148;
RA Overbeck S., Uciechowski P., Ackland M.L., Ford D., Rink L.;
RT "Intracellular zinc homeostasis in leukocyte subsets is regulated by
RT different expression of zinc exporters ZnT-1 to ZnT-9.";
RL J. Leukoc. Biol. 83:368-380(2008).
RN [11]
RP VARIANT TRP-325, AND INVOLVEMENT IN NONINSULIN-DEPENDENT DIABETES MELLITUS.
RX PubMed=17293876; DOI=10.1038/nature05616;
RA Sladek R., Rocheleau G., Rung J., Dina C., Shen L., Serre D., Boutin P.,
RA Vincent D., Belisle A., Hadjadj S., Balkau B., Heude B., Charpentier G.,
RA Hudson T.J., Montpetit A., Pshezhetsky A.V., Prentki M., Posner B.I.,
RA Balding D.J., Meyre D., Polychronakos C., Froguel P.;
RT "A genome-wide association study identifies novel risk loci for type 2
RT diabetes.";
RL Nature 445:881-885(2007).
CC -!- FUNCTION: Facilitates the accumulation of zinc from the cytoplasm into
CC intracellular vesicles, being a zinc-efflux transporter. May be a major
CC component for providing zinc to insulin maturation and/or storage
CC processes in insulin-secreting pancreatic beta-cells.
CC {ECO:0000269|PubMed:16984975}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q8IWU4; P16157-17: ANK1; NbExp=3; IntAct=EBI-10262251, EBI-941819;
CC Q8IWU4; Q13520: AQP6; NbExp=3; IntAct=EBI-10262251, EBI-13059134;
CC Q8IWU4; Q9NVJ2: ARL8B; NbExp=3; IntAct=EBI-10262251, EBI-718376;
CC Q8IWU4; Q9HD20-3: ATP13A1; NbExp=3; IntAct=EBI-10262251, EBI-12069500;
CC Q8IWU4; Q9BXK5: BCL2L13; NbExp=3; IntAct=EBI-10262251, EBI-747430;
CC Q8IWU4; Q13323: BIK; NbExp=3; IntAct=EBI-10262251, EBI-700794;
CC Q8IWU4; Q8WZ55: BSND; NbExp=3; IntAct=EBI-10262251, EBI-7996695;
CC Q8IWU4; P13236: CCL4; NbExp=3; IntAct=EBI-10262251, EBI-2873970;
CC Q8IWU4; Q8TCZ2: CD99L2; NbExp=3; IntAct=EBI-10262251, EBI-2824782;
CC Q8IWU4; Q99675: CGRRF1; NbExp=3; IntAct=EBI-10262251, EBI-2130213;
CC Q8IWU4; P56747: CLDN6; NbExp=3; IntAct=EBI-10262251, EBI-12955011;
CC Q8IWU4; Q96FZ5: CMTM7; NbExp=3; IntAct=EBI-10262251, EBI-2807956;
CC Q8IWU4; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-10262251, EBI-18013275;
CC Q8IWU4; Q96BA8: CREB3L1; NbExp=9; IntAct=EBI-10262251, EBI-6942903;
CC Q8IWU4; Q9BUN8: DERL1; NbExp=3; IntAct=EBI-10262251, EBI-398977;
CC Q8IWU4; Q15125: EBP; NbExp=3; IntAct=EBI-10262251, EBI-3915253;
CC Q8IWU4; Q9GZR5: ELOVL4; NbExp=3; IntAct=EBI-10262251, EBI-18535450;
CC Q8IWU4; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-10262251, EBI-781551;
CC Q8IWU4; P60508: ERVFRD-1; NbExp=3; IntAct=EBI-10262251, EBI-17973325;
CC Q8IWU4; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-10262251, EBI-18304435;
CC Q8IWU4; Q96KR6: FAM210B; NbExp=3; IntAct=EBI-10262251, EBI-18938272;
CC Q8IWU4; Q96IV6: FAXDC2; NbExp=3; IntAct=EBI-10262251, EBI-12142299;
CC Q8IWU4; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-10262251, EBI-12142257;
CC Q8IWU4; O60883: GPR37L1; NbExp=3; IntAct=EBI-10262251, EBI-2927498;
CC Q8IWU4; O15529: GPR42; NbExp=3; IntAct=EBI-10262251, EBI-18076404;
CC Q8IWU4; Q14416: GRM2; NbExp=3; IntAct=EBI-10262251, EBI-10232876;
CC Q8IWU4; Q9NP66: HMG20A; NbExp=3; IntAct=EBI-10262251, EBI-740641;
CC Q8IWU4; P30519: HMOX2; NbExp=3; IntAct=EBI-10262251, EBI-712096;
CC Q8IWU4; Q8NBQ5: HSD17B11; NbExp=3; IntAct=EBI-10262251, EBI-1052304;
CC Q8IWU4; Q96MG2: JSRP1; NbExp=3; IntAct=EBI-10262251, EBI-11305455;
CC Q8IWU4; A8MZ59: LEUTX; NbExp=3; IntAct=EBI-10262251, EBI-17490413;
CC Q8IWU4; P15941-11: MUC1; NbExp=3; IntAct=EBI-10262251, EBI-17263240;
CC Q8IWU4; Q16617: NKG7; NbExp=3; IntAct=EBI-10262251, EBI-3919611;
CC Q8IWU4; Q9P0S3: ORMDL1; NbExp=3; IntAct=EBI-10262251, EBI-1054848;
CC Q8IWU4; Q9NUU6: OTULINL; NbExp=3; IntAct=EBI-10262251, EBI-6916492;
CC Q8IWU4; P12004: PCNA; NbExp=3; IntAct=EBI-10262251, EBI-358311;
CC Q8IWU4; O15173: PGRMC2; NbExp=3; IntAct=EBI-10262251, EBI-1050125;
CC Q8IWU4; Q99640-2: PKMYT1; NbExp=3; IntAct=EBI-10262251, EBI-12257782;
CC Q8IWU4; P26678: PLN; NbExp=3; IntAct=EBI-10262251, EBI-692836;
CC Q8IWU4; P43378: PTPN9; NbExp=3; IntAct=EBI-10262251, EBI-742898;
CC Q8IWU4; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-10262251, EBI-7545592;
CC Q8IWU4; Q96HR9-2: REEP6; NbExp=3; IntAct=EBI-10262251, EBI-14065960;
CC Q8IWU4; Q8NC24: RELL2; NbExp=3; IntAct=EBI-10262251, EBI-10269209;
CC Q8IWU4; Q96TC7: RMDN3; NbExp=3; IntAct=EBI-10262251, EBI-1056589;
CC Q8IWU4; O95197-3: RTN3; NbExp=3; IntAct=EBI-10262251, EBI-11525735;
CC Q8IWU4; Q96GQ5: RUSF1; NbExp=3; IntAct=EBI-10262251, EBI-8636004;
CC Q8IWU4; Q9NR31: SAR1A; NbExp=3; IntAct=EBI-10262251, EBI-3920694;
CC Q8IWU4; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-10262251, EBI-17247926;
CC Q8IWU4; Q9Y6D0: SELENOK; NbExp=3; IntAct=EBI-10262251, EBI-9679163;
CC Q8IWU4; Q8WV19: SFT2D1; NbExp=3; IntAct=EBI-10262251, EBI-2854842;
CC Q8IWU4; O95562: SFT2D2; NbExp=3; IntAct=EBI-10262251, EBI-4402330;
CC Q8IWU4; P54219-3: SLC18A1; NbExp=3; IntAct=EBI-10262251, EBI-17595455;
CC Q8IWU4; Q99726: SLC30A3; NbExp=3; IntAct=EBI-10262251, EBI-10294651;
CC Q8IWU4; Q9NP94: SLC39A2; NbExp=3; IntAct=EBI-10262251, EBI-12898013;
CC Q8IWU4; Q9NPE6: SPAG4; NbExp=3; IntAct=EBI-10262251, EBI-10819434;
CC Q8IWU4; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-10262251, EBI-17280858;
CC Q8IWU4; Q8N2H4: SYS1; NbExp=3; IntAct=EBI-10262251, EBI-13075176;
CC Q8IWU4; Q8N9I0: SYT2; NbExp=3; IntAct=EBI-10262251, EBI-8032987;
CC Q8IWU4; Q8WY91: THAP4; NbExp=3; IntAct=EBI-10262251, EBI-726691;
CC Q8IWU4; C9JKN6: THSD7B; NbExp=3; IntAct=EBI-10262251, EBI-17192156;
CC Q8IWU4; P17152: TMEM11; NbExp=3; IntAct=EBI-10262251, EBI-723946;
CC Q8IWU4; Q8IV31: TMEM139; NbExp=3; IntAct=EBI-10262251, EBI-7238458;
CC Q8IWU4; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-10262251, EBI-8638294;
CC Q8IWU4; Q9BTX3: TMEM208; NbExp=3; IntAct=EBI-10262251, EBI-12876824;
CC Q8IWU4; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-10262251, EBI-10982110;
CC Q8IWU4; O95807: TMEM50A; NbExp=3; IntAct=EBI-10262251, EBI-12903814;
CC Q8IWU4; Q4KMG9: TMEM52B; NbExp=3; IntAct=EBI-10262251, EBI-18178701;
CC Q8IWU4; Q96HE8: TMEM80; NbExp=3; IntAct=EBI-10262251, EBI-11742770;
CC Q8IWU4; Q9P0T7: TMEM9; NbExp=3; IntAct=EBI-10262251, EBI-723976;
CC Q8IWU4; O60636: TSPAN2; NbExp=3; IntAct=EBI-10262251, EBI-3914288;
CC Q8IWU4; P23763-3: VAMP1; NbExp=3; IntAct=EBI-10262251, EBI-12097582;
CC Q8IWU4; P63027: VAMP2; NbExp=3; IntAct=EBI-10262251, EBI-520113;
CC Q8IWU4; Q9BSR8: YIPF4; NbExp=3; IntAct=EBI-10262251, EBI-751253;
CC Q8IWU4; Q96EC8: YIPF6; NbExp=3; IntAct=EBI-10262251, EBI-751210;
CC Q8IWU4; Q5T4F4: ZFYVE27; NbExp=3; IntAct=EBI-10262251, EBI-3892947;
CC Q8IWU4; PRO_0000041304 [P08563]; Xeno; NbExp=2; IntAct=EBI-10262251, EBI-11477759;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC Note=Associated with insulin and glucagon secretory granules.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IWU4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IWU4-2; Sequence=VSP_024025;
CC -!- TISSUE SPECIFICITY: In the endocrine pancreas, expressed in insulin-
CC producing beta cells. Expressed at relatively high levels in
CC subcutaneous fat tissue from lean persons; much lower levels in
CC visceral fat, whether from lean or obese individuals, and in
CC subcutaneous fat tissue from obese individuals. Expressed in peripheral
CC blood mononuclear cells, including T-cells and B-cells, with great
CC variation among individuals ranging from negative to strongly positive.
CC {ECO:0000269|PubMed:15331542, ECO:0000269|PubMed:16158222,
CC ECO:0000269|PubMed:16984975, ECO:0000269|PubMed:17118530,
CC ECO:0000269|PubMed:17971500}.
CC -!- DOMAIN: Contains a histidine-rich region, HXXXXXHNH-motif, which is a
CC ligand for zinc. {ECO:0000250}.
CC -!- POLYMORPHISM: Variant Trp-325 is a risk factor that confers
CC susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM)
CC [MIM:125853].
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR EMBL; AY117411; AAM80562.1; -; mRNA.
DR EMBL; AK298294; BAG60552.1; -; mRNA.
DR EMBL; AL713790; CAD28545.1; -; mRNA.
DR EMBL; EF560713; ABQ59023.1; -; mRNA.
DR EMBL; AC027419; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084114; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471060; EAW91968.1; -; Genomic_DNA.
DR EMBL; BC126446; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS55272.1; -. [Q8IWU4-2]
DR CCDS; CCDS6322.1; -. [Q8IWU4-1]
DR RefSeq; NP_001166282.1; NM_001172811.1. [Q8IWU4-2]
DR RefSeq; NP_001166284.1; NM_001172813.1. [Q8IWU4-2]
DR RefSeq; NP_001166285.1; NM_001172814.1. [Q8IWU4-2]
DR RefSeq; NP_001166286.1; NM_001172815.2. [Q8IWU4-2]
DR RefSeq; NP_776250.2; NM_173851.2. [Q8IWU4-1]
DR PDB; 6XPD; EM; 3.80 A; A/B=50-369.
DR PDB; 6XPE; EM; 4.10 A; A/B=50-369.
DR PDB; 6XPF; EM; 5.90 A; A/B=50-369.
DR PDBsum; 6XPD; -.
DR PDBsum; 6XPE; -.
DR PDBsum; 6XPF; -.
DR AlphaFoldDB; Q8IWU4; -.
DR SMR; Q8IWU4; -.
DR BioGRID; 127975; 70.
DR IntAct; Q8IWU4; 76.
DR MINT; Q8IWU4; -.
DR STRING; 9606.ENSP00000415011; -.
DR DrugBank; DB14533; Zinc chloride.
DR DrugBank; DB14548; Zinc sulfate, unspecified form.
DR TCDB; 2.A.4.3.5; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q8IWU4; -.
DR PhosphoSitePlus; Q8IWU4; -.
DR BioMuta; SLC30A8; -.
DR DMDM; 190358866; -.
DR jPOST; Q8IWU4; -.
DR MassIVE; Q8IWU4; -.
DR PaxDb; Q8IWU4; -.
DR PeptideAtlas; Q8IWU4; -.
DR PRIDE; Q8IWU4; -.
DR ProteomicsDB; 70895; -. [Q8IWU4-1]
DR ProteomicsDB; 70896; -. [Q8IWU4-2]
DR Antibodypedia; 42965; 220 antibodies from 33 providers.
DR DNASU; 169026; -.
DR Ensembl; ENST00000427715.2; ENSP00000407505.2; ENSG00000164756.13. [Q8IWU4-2]
DR Ensembl; ENST00000456015.7; ENSP00000415011.2; ENSG00000164756.13. [Q8IWU4-1]
DR Ensembl; ENST00000519688.5; ENSP00000431069.1; ENSG00000164756.13. [Q8IWU4-2]
DR Ensembl; ENST00000521243.5; ENSP00000428545.1; ENSG00000164756.13. [Q8IWU4-2]
DR GeneID; 169026; -.
DR KEGG; hsa:169026; -.
DR MANE-Select; ENST00000456015.7; ENSP00000415011.2; NM_173851.3; NP_776250.2.
DR UCSC; uc003yog.3; human. [Q8IWU4-1]
DR CTD; 169026; -.
DR DisGeNET; 169026; -.
DR GeneCards; SLC30A8; -.
DR HGNC; HGNC:20303; SLC30A8.
DR HPA; ENSG00000164756; Tissue enriched (pancreas).
DR MalaCards; SLC30A8; -.
DR MIM; 125853; phenotype.
DR MIM; 611145; gene.
DR neXtProt; NX_Q8IWU4; -.
DR OpenTargets; ENSG00000164756; -.
DR PharmGKB; PA134915546; -.
DR VEuPathDB; HostDB:ENSG00000164756; -.
DR eggNOG; KOG1482; Eukaryota.
DR GeneTree; ENSGT00940000160706; -.
DR HOGENOM; CLU_013430_0_1_1; -.
DR InParanoid; Q8IWU4; -.
DR OMA; RATKMYA; -.
DR OrthoDB; 820567at2759; -.
DR PhylomeDB; Q8IWU4; -.
DR TreeFam; TF313382; -.
DR PathwayCommons; Q8IWU4; -.
DR Reactome; R-HSA-264876; Insulin processing.
DR Reactome; R-HSA-435368; Zinc efflux and compartmentalization by the SLC30 family.
DR SignaLink; Q8IWU4; -.
DR BioGRID-ORCS; 169026; 11 hits in 1070 CRISPR screens.
DR ChiTaRS; SLC30A8; human.
DR GeneWiki; SLC30A8; -.
DR GenomeRNAi; 169026; -.
DR Pharos; Q8IWU4; Tbio.
DR PRO; PR:Q8IWU4; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8IWU4; protein.
DR Bgee; ENSG00000164756; Expressed in islet of Langerhans and 92 other tissues.
DR ExpressionAtlas; Q8IWU4; baseline and differential.
DR Genevisible; Q8IWU4; HS.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISS:BHF-UCL.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:BHF-UCL.
DR GO; GO:0030141; C:secretory granule; IDA:BHF-UCL.
DR GO; GO:0030667; C:secretory granule membrane; TAS:Reactome.
DR GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:BHF-UCL.
DR GO; GO:0008270; F:zinc ion binding; IC:BHF-UCL.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IDA:BHF-UCL.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; ISS:BHF-UCL.
DR GO; GO:0030070; P:insulin processing; TAS:Reactome.
DR GO; GO:0030073; P:insulin secretion; IMP:BHF-UCL.
DR GO; GO:0032024; P:positive regulation of insulin secretion; ISS:BHF-UCL.
DR GO; GO:0061088; P:regulation of sequestering of zinc ion; ISS:BHF-UCL.
DR GO; GO:0060627; P:regulation of vesicle-mediated transport; ISS:BHF-UCL.
DR GO; GO:0009749; P:response to glucose; IMP:BHF-UCL.
DR GO; GO:0034341; P:response to interferon-gamma; IEA:Ensembl.
DR GO; GO:0070555; P:response to interleukin-1; IEA:Ensembl.
DR GO; GO:0010043; P:response to zinc ion; IBA:GO_Central.
DR GO; GO:0032119; P:sequestering of zinc ion; IDA:BHF-UCL.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IDA:BHF-UCL.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR033572; ZnT-8.
DR PANTHER; PTHR11562:SF37; PTHR11562:SF37; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Cytoplasmic vesicle;
KW Diabetes mellitus; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Zinc; Zinc transport.
FT CHAIN 1..369
FT /note="Zinc transporter 8"
FT /id="PRO_0000281740"
FT TOPO_DOM 1..79
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..245
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 31..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 197..205
FT /note="HXXXXX[HY]NH-motif"
FT COMPBIAS 33..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..49
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005"
FT /id="VSP_024025"
FT VARIANT 325
FT /note="R -> Q (in dbSNP:rs16889462)"
FT /id="VAR_031258"
FT VARIANT 325
FT /note="R -> W (in dbSNP:rs13266634)"
FT /evidence="ECO:0000269|PubMed:17293876"
FT /id="VAR_031259"
FT CONFLICT 18
FT /note="Y -> H (in Ref. 1; AAM80562)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="L -> S (in Ref. 3; ABQ59023)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="L -> P (in Ref. 3; CAD28545)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="S -> C (in Ref. 3; CAD28545)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 369 AA; 40755 MW; 45926FADCEE18FF3 CRC64;
MEFLERTYLV NDKAAKMYAF TLESVELQQK PVNKDQCPRE RPEELESGGM YHCHSGSKPT
EKGANEYAYA KWKLCSASAI CFIFMIAEVV GGHIAGSLAV VTDAAHLLID LTSFLLSLFS
LWLSSKPPSK RLTFGWHRAE ILGALLSILC IWVVTGVLVY LACERLLYPD YQIQATVMII
VSSCAVAANI VLTVVLHQRC LGHNHKEVQA NASVRAAFVH ALGDLFQSIS VLISALIIYF
KPEYKIADPI CTFIFSILVL ASTITILKDF SILLMEGVPK SLNYSGVKEL ILAVDGVLSV
HSLHIWSLTM NQVILSAHVA TAASRDSQVV RREIAKALSK SFTMHSLTIQ MESPVDQDPD
CLFCEDPCD
