ID   ZNT8_XENLA              Reviewed;         375 AA.
AC   Q5I020;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Zinc transporter 8;
DE            Short=ZnT-8;
DE   AltName: Full=Solute carrier family 30 member 8;
GN   Name=slc30a8;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Facilitates the accumulation of zinc from the cytoplasm into
CC       intracellular vesicles, being a zinc-efflux transporter. Involved in
CC       zinc ion homeostasis and cellular distribution (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Contains a histidine-rich region, HXXXXXHNH-motif, which is a
CC       ligand for zinc. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC       transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR   EMBL; BC088803; AAH88803.1; -; mRNA.
DR   RefSeq; NP_001088922.1; NM_001095453.1.
DR   AlphaFoldDB; Q5I020; -.
DR   SMR; Q5I020; -.
DR   DNASU; 496294; -.
DR   GeneID; 496294; -.
DR   CTD; 496294; -.
DR   Xenbase; XB-GENE-958130; slc30a8.L.
DR   OrthoDB; 973492at2759; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   Bgee; 496294; Expressed in neurula embryo and 7 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005385; F:zinc ion transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006882; P:cellular zinc ion homeostasis; IEA:InterPro.
DR   Gene3D; 1.20.1510.10; -; 1.
DR   Gene3D; 3.30.70.1350; -; 1.
DR   InterPro; IPR002524; Cation_efflux.
DR   InterPro; IPR036837; Cation_efflux_CTD_sf.
DR   InterPro; IPR027469; Cation_efflux_TMD_sf.
DR   InterPro; IPR033572; ZnT-8.
DR   PANTHER; PTHR11562:SF37; PTHR11562:SF37; 1.
DR   Pfam; PF01545; Cation_efflux; 1.
DR   SUPFAM; SSF160240; SSF160240; 1.
DR   SUPFAM; SSF161111; SSF161111; 1.
DR   TIGRFAMs; TIGR01297; CDF; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasmic vesicle; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW   Zinc transport.
FT   CHAIN           1..375
FT                   /note="Zinc transporter 8"
FT                   /id="PRO_0000281742"
FT   TOPO_DOM        1..68
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        90..98
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        120..135
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        157..170
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        192..223
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        245..251
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        273..375
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOTIF           192..200
FT                   /note="HXXXXX[HY]NH-motif"
SQ   SEQUENCE   375 AA;  41422 MW;  734E8570AAA068D3 CRC64;
     MKGPEKAYLV SDKATKMYSL TMDSSEKNNC GKPPLQDDEN PHIKYHCHNN NTKAYDARQR
     EQTSAKKKLC IASLICFVFI SAEIVGGYIA GSLAVVTDAA HLLVDLSSFF ISLGSLWLSS
     KSSTMRLTFG WYRAEILGAL MSIITIWLVT GVLVYLAIER IIRPDYTIDG TVMLITSACA
     LGANVVLALI LHQSGHGHSH AGGKHEHMAS EYKPQTNASI RAAFIHVIGD LFQSISVLIS
     ALIIYFKPEY KIADPICTFI FSIFVLITTV TVLRDLLNIL MEGTPRGIHY SDVKQSILAV
     DGVKSVHSLH LWALTMNQVI LSAHIATDIL GESKRILKDV TQNVCSSFPF HSVTIQVEPV
     EEQSPECMFC YEPTQ