ID   ZNT8_XENTR              Reviewed;         374 AA.
AC   Q5XHB4;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Zinc transporter 8;
DE            Short=ZnT-8;
DE   AltName: Full=Solute carrier family 30 member 8;
GN   Name=slc30a8;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Facilitates the accumulation of zinc from the cytoplasm into
CC       intracellular vesicles, being a zinc-efflux transporter. Involved in
CC       zinc ion homeostasis and cellular distribution (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Contains a histidine-rich region, HXXXXXHNH-motif, which is a
CC       ligand for zinc. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC       transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR   EMBL; BC084148; AAH84148.1; -; mRNA.
DR   RefSeq; NP_001011041.1; NM_001011041.1.
DR   AlphaFoldDB; Q5XHB4; -.
DR   SMR; Q5XHB4; -.
DR   GeneID; 496450; -.
DR   KEGG; xtr:496450; -.
DR   CTD; 169026; -.
DR   Xenbase; XB-GENE-958127; slc30a8.
DR   InParanoid; Q5XHB4; -.
DR   OrthoDB; 820567at2759; -.
DR   Reactome; R-XTR-264876; Insulin processing.
DR   Reactome; R-XTR-435368; Zinc efflux and compartmentalization by the SLC30 family.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006882; P:cellular zinc ion homeostasis; IEA:InterPro.
DR   GO; GO:0030073; P:insulin secretion; IBA:GO_Central.
DR   GO; GO:0061088; P:regulation of sequestering of zinc ion; IBA:GO_Central.
DR   GO; GO:0009749; P:response to glucose; IBA:GO_Central.
DR   GO; GO:0010043; P:response to zinc ion; IBA:GO_Central.
DR   GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1510.10; -; 1.
DR   InterPro; IPR002524; Cation_efflux.
DR   InterPro; IPR036837; Cation_efflux_CTD_sf.
DR   InterPro; IPR027469; Cation_efflux_TMD_sf.
DR   InterPro; IPR033572; ZnT-8.
DR   PANTHER; PTHR11562:SF37; PTHR11562:SF37; 1.
DR   Pfam; PF01545; Cation_efflux; 1.
DR   SUPFAM; SSF160240; SSF160240; 1.
DR   SUPFAM; SSF161111; SSF161111; 1.
DR   TIGRFAMs; TIGR01297; CDF; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasmic vesicle; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW   Zinc transport.
FT   CHAIN           1..374
FT                   /note="Zinc transporter 8"
FT                   /id="PRO_0000281743"
FT   TOPO_DOM        1..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        89..91
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        113..134
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        156..169
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        191..222
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        244..251
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        273..374
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          18..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           191..199
FT                   /note="HXXXXX[HY]NH-motif"
SQ   SEQUENCE   374 AA;  41490 MW;  E862C8BEE5E88FD8 CRC64;
     MKGSEEAYLV SDKATKMYSL TKDSEKNHPS KPPLQDEENP QSKYHCHNNN KKAYDARQRE
     QTFAKKKLCI ASLICFVFIS AEIVGGYIAG SLAVVTDAAH LLVDLSSFFI SLCSLWLSSK
     SSTTRLTFGW HRAEILGALM SVITIWLVTG VLVYLACERL IRPDYTIDGT VMLITSACAL
     GANLVLALIL HQSGHGHSHA GGKHEHMASE YKPQTNASIR AAFIHVIGDL FQSISVLISA
     LIIYFKPEYK MADPICTFIF SIFVLITTVT VLRDLLTVLM EGTPRGIHYS DVKQSILAVD
     GVKSVHSLHL WALTMNQVIL SAHIATDIVG ESKRILKDVT QNVFARFPFH SVTIQVEPIE
     DQSPECMFCY EPTQ