ID   ZNTB_VIBPA              Reviewed;         315 AA.
AC   Q87M69;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Zinc transport protein ZntB {ECO:0000305};
GN   Name=zntB {ECO:0000303|PubMed:19653298};
GN   OrderedLocusNames=VP2389 {ECO:0000312|EMBL:BAC60652.1};
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
RN   [2] {ECO:0007744|PDB:3CK6}
RP   X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-249 IN COMPLEX WITH CHLORIDE
RP   IONS, SUBUNIT, AND DOMAIN.
RC   STRAIN=RIMD 2210633;
RX   PubMed=19653298; DOI=10.1002/pro.215;
RA   Tan K., Sather A., Robertson J.L., Moy S., Roux B., Joachimiak A.;
RT   "Structure and electrostatic property of cytoplasmic domain of ZntB
RT   transporter.";
RL   Protein Sci. 18:2043-2052(2009).
CC   -!- FUNCTION: Zinc transporter. Acts as a Zn(2+):proton symporter, which
CC       likely mediates zinc ion uptake. {ECO:0000250|UniProtKB:P64423}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(out) + Zn(2+)(out) = H(+)(in) + Zn(2+)(in);
CC         Xref=Rhea:RHEA:71195, ChEBI:CHEBI:15378, ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:P64423};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71196;
CC         Evidence={ECO:0000250|UniProtKB:P64423};
CC   -!- SUBUNIT: Homopentamer (PubMed:19653298). Can assemble pentamers in the
CC       absence of the transmembrane regions (PubMed:19653298).
CC       {ECO:0000269|PubMed:19653298}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P64423}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DOMAIN: The intracellular cytoplasmic domain forms a funnel-shaped
CC       homopentamer. 25 well-defined Cl(-) ions were observed and some of
CC       these binding sites are highly conserved within the ZntB family. The
CC       presence of the bound Cl(-) ions increases the stability of cations
CC       along the pore suggesting they could be important in enhancing cation
CC       transport. {ECO:0000269|PubMed:19653298}.
CC   -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35)
CC       family. {ECO:0000305}.
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DR   EMBL; BA000031; BAC60652.1; -; Genomic_DNA.
DR   RefSeq; NP_798768.1; NC_004603.1.
DR   RefSeq; WP_005456583.1; NC_004603.1.
DR   PDB; 3CK6; X-ray; 1.90 A; A/B/C/D/E=1-249.
DR   PDBsum; 3CK6; -.
DR   SMR; Q87M69; -.
DR   STRING; 223926.28807387; -.
DR   TCDB; 1.A.35.4.2; the cora metal ion transporter (mit) family.
DR   EnsemblBacteria; BAC60652; BAC60652; BAC60652.
DR   GeneID; 1189902; -.
DR   KEGG; vpa:VP2389; -.
DR   PATRIC; fig|223926.6.peg.2291; -.
DR   eggNOG; COG0598; Bacteria.
DR   HOGENOM; CLU_007127_2_0_6; -.
DR   OMA; MVSVRIF; -.
DR   EvolutionaryTrace; Q87M69; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046873; F:metal ion transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR045861; CorA_cytoplasmic_dom.
DR   InterPro; IPR045863; CorA_TM1_TM2.
DR   InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR   Pfam; PF01544; CorA; 1.
DR   SUPFAM; SSF143865; SSF143865; 1.
DR   SUPFAM; SSF144083; SSF144083; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc.
FT   CHAIN           1..315
FT                   /note="Zinc transport protein ZntB"
FT                   /id="PRO_0000455826"
FT   TOPO_DOM        1..250
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        251..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        272..282
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        283..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        304..315
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   315 AA;  36354 MW;  0ED153FDEAAEB6FC CRC64;
     MGFMIEHWDF STPMATQETT TAEHIQPNHW YHCERLHPDI RSWLEDNHVP RATVDHLLAD
     ESRPSFHPLD DDNFMLILRG INMNENASPE DMLSIRILYF QGALISTRKI PSRAIMEIRQ
     ALAEHKGPKS LASLLNQIIE GLNGKIDLYL DTIEETLNEF DVNDESTYNH IAAQKALISI
     KRFIRPQQYA IRDLIESESE LVTSRPHQYR FAHNNITRIN ETIEFYLGEV ALFQDEIKHN
     RDEKTNKNSY LFTLVATIFL PTSFLTGLLG INIGGMPGVE SSMAFTWFCI ALIVIFGLEW
     LLFKRLGFTN KTDDE