ID   ZNUA_BRUA2              Reviewed;         334 AA.
AC   Q2YJH5; Q58J93;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=High-affinity zinc uptake system protein ZnuA;
DE   Flags: Precursor;
GN   Name=znuA; OrderedLocusNames=BAB2_1079;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16790759; DOI=10.1128/iai.01957-05;
RA   Yang X., Becker T., Walters N., Pascual D.W.;
RT   "Deletion of znuA virulence factor attenuates Brucella abortus and confers
RT   protection against wild-type challenge.";
RL   Infect. Immun. 74:3874-3879(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
CC   -!- FUNCTION: Involved in the high-affinity zinc uptake transport system.
CC       Required for survival and normal growth under low Zn (2+)
CC       concentrations. Also required for virulence and intracellular growth in
CC       macrophages. {ECO:0000269|PubMed:16790759}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Mutant strain (znuA delection) is attenuated in
CC       infected mice, showing decreased splenic CFU. Also confers a protective
CC       efficacy similar to that of RB51 and S19 vaccine strains.
CC       {ECO:0000269|PubMed:16790759}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 9 family.
CC       {ECO:0000305}.
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DR   EMBL; AY941821; AAX46323.1; -; Genomic_DNA.
DR   EMBL; AM040265; CAJ13245.1; -; Genomic_DNA.
DR   RefSeq; WP_002966661.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YJH5; -.
DR   SMR; Q2YJH5; -.
DR   STRING; 359391.BAB2_1079; -.
DR   EnsemblBacteria; CAJ13245; CAJ13245; BAB2_1079.
DR   GeneID; 3827700; -.
DR   KEGG; bmf:BAB2_1079; -.
DR   PATRIC; fig|359391.11.peg.1863; -.
DR   HOGENOM; CLU_016838_1_2_5; -.
DR   OMA; FHEAFDY; -.
DR   PhylomeDB; Q2YJH5; -.
DR   PRO; PR:Q2YJH5; -.
DR   Proteomes; UP000002719; Chromosome II.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR   CDD; cd01019; ZnuA; 1.
DR   InterPro; IPR035520; ZnuA.
DR   InterPro; IPR006127; ZnuA-like.
DR   Pfam; PF01297; ZnuA; 1.
PE   3: Inferred from homology;
KW   Ion transport; Metal-binding; Periplasm; Reference proteome; Signal;
KW   Transport; Virulence; Zinc; Zinc transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..334
FT                   /note="High-affinity zinc uptake system protein ZnuA"
FT                   /id="PRO_0000248947"
FT   REGION          120..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         61
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        266
FT                   /note="I -> T (in Ref. 1; AAX46323)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   334 AA;  36046 MW;  F3C873631569099F CRC64;
     MKNLHSLFLA SAFLAGFCGS SLAGEREGVV VSIKPLHSIV SAVMQGVGKP KLIVQGAGSE
     HVYSLKPSDA EAIEHAKVIF WAGPSMETFL DKPIDTLGEG AKVVALGDAK GLTKLKFREG
     GPFEAHDHGH GGSHEEEHDA HGSGDHDHAA EVAEEGHEHH HHGEYDLHFW LDPQNGKILA
     ADIAKTLGES DPEHAAQYEK NAKAYGEKLD ALTREVAAEL KPVKDKPFIV FHDAYQYFEN
     RFGMKAAGSI TVSPEKAPGA ARIQQIHDKI KSLGATCVFS EPQFEPKLVK TVVDGTKART
     GVLDPLGAEL KDGPDLYPQL IRNLANSLKD CLPK