ID   ZNUA_BRUAB              Reviewed;         334 AA.
AC   Q576K1;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=High-affinity zinc uptake system protein ZnuA;
DE   Flags: Precursor;
GN   Name=znuA; OrderedLocusNames=BruAb2_1059;
OS   Brucella abortus biovar 1 (strain 9-941).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=262698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=9-941;
RX   PubMed=15805518; DOI=10.1128/jb.187.8.2715-2726.2005;
RA   Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z.,
RA   Li L.-L., Kapur V., Alt D.P., Olsen S.C.;
RT   "Completion of the genome sequence of Brucella abortus and comparison to
RT   the highly similar genomes of Brucella melitensis and Brucella suis.";
RL   J. Bacteriol. 187:2715-2726(2005).
RN   [2]
RP   FUNCTION.
RC   STRAIN=544 / Biovar 1;
RX   PubMed=15472468; DOI=10.1292/jvms.66.1059;
RA   Kim S., Watanabe K., Shirahata T., Watarai M.;
RT   "Zinc uptake system (znuA locus) of Brucella abortus is essential for
RT   intracellular survival and virulence in mice.";
RL   J. Vet. Med. Sci. 66:1059-1063(2004).
CC   -!- FUNCTION: Involved in the high-affinity zinc uptake transport system.
CC       Required for survival and normal growth under low Zn (2+)
CC       concentrations. Also required for virulence and intracellular growth in
CC       macrophages. {ECO:0000269|PubMed:15472468}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 9 family.
CC       {ECO:0000305}.
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DR   EMBL; AE017224; AAX76433.1; -; Genomic_DNA.
DR   RefSeq; WP_002966661.1; NC_006933.1.
DR   AlphaFoldDB; Q576K1; -.
DR   SMR; Q576K1; -.
DR   PRIDE; Q576K1; -.
DR   EnsemblBacteria; AAX76433; AAX76433; BruAb2_1059.
DR   GeneID; 3827700; -.
DR   KEGG; bmb:BruAb2_1059; -.
DR   HOGENOM; CLU_016838_1_2_5; -.
DR   OMA; FHEAFDY; -.
DR   PRO; PR:Q576K1; -.
DR   Proteomes; UP000000540; Chromosome II.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR   CDD; cd01019; ZnuA; 1.
DR   InterPro; IPR035520; ZnuA.
DR   InterPro; IPR006127; ZnuA-like.
DR   Pfam; PF01297; ZnuA; 1.
PE   3: Inferred from homology;
KW   Ion transport; Metal-binding; Periplasm; Signal; Transport; Virulence;
KW   Zinc; Zinc transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..334
FT                   /note="High-affinity zinc uptake system protein ZnuA"
FT                   /id="PRO_0000248944"
FT   REGION          120..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         61
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   334 AA;  36046 MW;  F3C873631569099F CRC64;
     MKNLHSLFLA SAFLAGFCGS SLAGEREGVV VSIKPLHSIV SAVMQGVGKP KLIVQGAGSE
     HVYSLKPSDA EAIEHAKVIF WAGPSMETFL DKPIDTLGEG AKVVALGDAK GLTKLKFREG
     GPFEAHDHGH GGSHEEEHDA HGSGDHDHAA EVAEEGHEHH HHGEYDLHFW LDPQNGKILA
     ADIAKTLGES DPEHAAQYEK NAKAYGEKLD ALTREVAAEL KPVKDKPFIV FHDAYQYFEN
     RFGMKAAGSI TVSPEKAPGA ARIQQIHDKI KSLGATCVFS EPQFEPKLVK TVVDGTKART
     GVLDPLGAEL KDGPDLYPQL IRNLANSLKD CLPK