ID   ZNUA_BRUME              Reviewed;         334 AA.
AC   Q8YDJ7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=High-affinity zinc uptake system protein ZnuA;
DE   Flags: Precursor;
GN   Name=znuA; OrderedLocusNames=BMEII0178;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Involved in the high-affinity zinc uptake transport system.
CC       Required for survival and normal growth under low Zn (2+)
CC       concentrations. Also required for virulence and intracellular growth in
CC       host cells (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 9 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL53419.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE008918; AAL53419.1; ALT_INIT; Genomic_DNA.
DR   PIR; AH3531; AH3531.
DR   RefSeq; WP_004680953.1; NZ_GG703779.1.
DR   AlphaFoldDB; Q8YDJ7; -.
DR   SMR; Q8YDJ7; -.
DR   STRING; 224914.BMEII0178; -.
DR   EnsemblBacteria; AAL53419; AAL53419; BMEII0178.
DR   GeneID; 45126384; -.
DR   KEGG; bme:BMEII0178; -.
DR   PATRIC; fig|224914.52.peg.3211; -.
DR   eggNOG; COG4531; Bacteria.
DR   OMA; FHEAFDY; -.
DR   PhylomeDB; Q8YDJ7; -.
DR   PRO; PR:Q8YDJ7; -.
DR   Proteomes; UP000000419; Chromosome II.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR   CDD; cd01019; ZnuA; 1.
DR   InterPro; IPR035520; ZnuA.
DR   InterPro; IPR006127; ZnuA-like.
DR   Pfam; PF01297; ZnuA; 1.
PE   3: Inferred from homology;
KW   Ion transport; Metal-binding; Periplasm; Signal; Transport; Virulence;
KW   Zinc; Zinc transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..334
FT                   /note="High-affinity zinc uptake system protein ZnuA"
FT                   /id="PRO_0000248945"
FT   REGION          120..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         61
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   334 AA;  36036 MW;  F3CAA3631569099F CRC64;
     MKNLHSLFLA SAFLAGFCGS SLAGEREGVV VSIKPLHSIV SAVMQGVGKP KLIVQGAGSE
     HVYSLKPSDA EAIEHAKVIF WAGPSMETFL DKPIDTLGEG AKVVALGDAK GLTKLKFREG
     GPFEAHDHGH GGSHEEEHDA HGSGDHDHAA EVAEEGHEHH HHGEYDLHFW LDPQNGKILA
     ADIAKTLGES DPEHAAQYEK NAKAYGEKLD ALTREVAAEL KPVKDKPFIV FHDAYQYFEN
     RFGMKAAGSI TVSPEKAPGA ARIQQIHDKI KSLGATCVFS EPQFEPKLVK TVVDGTKART
     GVLDPLGAEL KDGPDLYPQL IRNLANSLKD CLSK