ZNUB_ECOLI
ID ZNUB_ECOLI Reviewed; 261 AA.
AC P39832; P76286;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=High-affinity zinc uptake system membrane protein ZnuB;
GN Name=znuB; Synonyms=yebI; OrderedLocusNames=b1859, JW1848;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
RC STRAIN=K12 / EMG2;
RA Robison K., O'Keeffe T., Church G.M.;
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-261.
RX PubMed=2842314; DOI=10.1128/jb.170.9.4322-4329.1988;
RA Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.;
RT "Structure and regulation of the Escherichia coli ruv operon involved in
RT DNA repair and recombination.";
RL J. Bacteriol. 170:4322-4329(1988).
RN [6]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [7]
RP CHARACTERIZATION.
RX PubMed=9680209; DOI=10.1046/j.1365-2958.1998.00883.x;
RA Patzer S.I., Hantke K.;
RT "The ZnuABC high-affinity zinc uptake system and its regulator Zur in
RT Escherichia coli.";
RL Mol. Microbiol. 28:1199-1210(1998).
RN [8]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the high-affinity zinc uptake transport system.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC-3 integral membrane protein family.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC74929.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15667.1; -; Genomic_DNA.
DR EMBL; U38702; AAA81031.1; -; Genomic_DNA.
DR EMBL; M21298; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; C64948; C64948.
DR RefSeq; NP_416373.1; NC_000913.3.
DR RefSeq; WP_000571480.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P39832; -.
DR SMR; P39832; -.
DR BioGRID; 4260881; 11.
DR ComplexPortal; CPX-4407; Zinc ABC transporter complex.
DR STRING; 511145.b1859; -.
DR TCDB; 3.A.1.15.5; the atp-binding cassette (abc) superfamily.
DR PaxDb; P39832; -.
DR PRIDE; P39832; -.
DR EnsemblBacteria; AAC74929; AAC74929; b1859.
DR EnsemblBacteria; BAA15667; BAA15667; BAA15667.
DR GeneID; 946372; -.
DR KEGG; ecj:JW1848; -.
DR KEGG; eco:b1859; -.
DR PATRIC; fig|1411691.4.peg.389; -.
DR EchoBASE; EB2271; -.
DR eggNOG; COG1108; Bacteria.
DR HOGENOM; CLU_028808_3_2_6; -.
DR InParanoid; P39832; -.
DR OMA; RIDLMAY; -.
DR PhylomeDB; P39832; -.
DR BioCyc; EcoCyc:ZNUB-MON; -.
DR BioCyc; MetaCyc:ZNUB-MON; -.
DR PRO; PR:P39832; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0000006; F:high-affinity zinc transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0010043; P:response to zinc ion; IBA:GO_Central.
DR GO; GO:0055069; P:zinc ion homeostasis; IC:ComplexPortal.
DR GO; GO:0071578; P:zinc ion import across plasma membrane; IDA:EcoCyc.
DR Gene3D; 1.10.3470.10; -; 1.
DR InterPro; IPR037294; ABC_BtuC-like.
DR InterPro; IPR001626; ABC_TroCD.
DR PANTHER; PTHR30477; PTHR30477; 1.
DR Pfam; PF00950; ABC-3; 1.
DR SUPFAM; SSF81345; SSF81345; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW Zinc transport.
FT CHAIN 1..261
FT /note="High-affinity zinc uptake system membrane protein
FT ZnuB"
FT /id="PRO_0000171161"
FT TOPO_DOM 1..7
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..83
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..177
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 261 AA; 27729 MW; 78382B2EAECE1490 CRC64;
MIELLFPGWL AGIMLACAAG PLGSFVVWRR MSYFGDTLAH ASLLGVAFGL LLDVNPFYAV
IAVTLLLAGG LVWLEKRPQL AIDTLLGIMA HSALSLGLVV VSLMSNIRVD LMAYLFGDLL
AVTPEDLISI AIGVVIVVAI LFWQWRNLLS MTISPDLAFV DGVKLQRVKL LLMLVTALTI
GVAMKFVGAL IITSLLIIPA ATARRFARTP EQMAGVAVLV GMVAVTGGLT FSAVYDTPAG
PSVVLCAALL FILSMMKKQA S
