ID   ZORA_ECO24              Reviewed;         729 AA.
AC   A7ZI10;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 2.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Zorya protein ZorA {ECO:0000303|PubMed:29371424};
GN   Name=zorA {ECO:0000303|PubMed:29371424};
GN   OrderedLocusNames=EcE24377A_0285 {ECO:0000312|EMBL:ABV17222.1};
OS   Escherichia coli O139:H28 (strain E24377A / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331111;
RN   [1] {ECO:0000312|EMBL:ABV17222.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E24377A / ETEC;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP   THR-147 AND SER-184.
RC   STRAIN=E24377A / ETEC;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Zorya type I, composed
CC       of ZorA, ZorB, ZorC and ZorD. Expression of Zorya type I in E.coli
CC       (strain MG1655) confers 10,000-fold resistance to phage SECphi27, 100-
CC       fold resistance to lambda, and 10-fold resistance to T7. While most T7
CC       infected Zorya-containing cells undergo abortive infection, a minority
CC       produce viable phage progeny. These eventually accumulate to a high
CC       multiplicity of infection, leading to culture collapse by 2 hours after
CC       initial infection (PubMed:29371424). ZorA and ZorB probably assemble in
CC       the cell inner membrane and exert their effect there (Probable).
CC       {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:29371424}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Zorya system does
CC       not confer resistance to SECphi27 in E.coli.
CC       {ECO:0000269|PubMed:29371424}.
CC   -!- SIMILARITY: Belongs to the MotA family. {ECO:0000305|PubMed:29371424}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV17222.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:29371424};
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DR   EMBL; CP000800; ABV17222.1; ALT_INIT; Genomic_DNA.
DR   EnsemblBacteria; ABV17222; ABV17222; EcE24377A_0285.
DR   KEGG; ecw:EcE24377A_0285; -.
DR   HOGENOM; CLU_022865_0_0_6; -.
DR   OMA; FGGQMRG; -.
DR   Proteomes; UP000001122; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..729
FT                   /note="Zorya protein ZorA"
FT                   /id="PRO_0000456338"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         147
FT                   /note="T->A: No longer resistant to SECphi27; when
FT                   associated with A-184."
FT                   /evidence="ECO:0000269|PubMed:29371424"
FT   MUTAGEN         184
FT                   /note="S->A: No longer resistant to SECphi27; when
FT                   associated with A-147."
FT                   /evidence="ECO:0000269|PubMed:29371424"
SQ   SEQUENCE   729 AA;  80261 MW;  E17594C6170F03A5 CRC64;
     MSWLNSVLLA LTSVQPYMVP ATVIGLVSFA FLCFIFFYFF RAVKIINGLK KYTQSINGIE
     NNEPGNQLQH LQSLFVQPEL KHAWNEFEES LHSQYELEDG EEKIVRIRAT APSASFFSEQ
     QLVDIPLNTE FFKHLPGILT GVGIIGTFYG LMIGLNHFDP STPEQVSSSV NNLLRDVLYA
     FLGSAFAITF SILITWLEKF CLAKCYKYLE KFTAALDALY DSGVGEEYLA SLVKSSNESA
     TQARHLKESL VTDLRDMLLH LANSQKVENE RLATTLSTTY RETGQQFAEQ VSGAIENSLK
     SPLDKIAGAV QTASGDQSGM VQNMLQDVLT AFMAKLDTTF GQQFTNLNEM MGQTVGAIQT
     MQTGFGALLQ DMRQVSDDSR QGSAQLIEQL LSEMKSGQQA MQAGMNDMLT SLQTSVAKIG
     AEGEGAGERM ARQLEKMFAD SEAREKAQAE HMTAFIEAIQ NSVQQGQSAT MEKMAASVES
     LGEQLGSLFG QIDKGQQQIS ANQQANQQSL HEQTQRVMSE VDDQIKQLVE TVASQHQGTT
     ETLRLLAEQT NRQIQDMHTG ADKMRLAAER FEHAGDRVSE ANHLTADVLN KAQSAGSSLS
     LATSELTSVV ADYRNNREAV SKSIAMLELL AANTQSEQTT RTQFIADLKQ HGERLQSYNR
     EAQAFMENVS DVLGKGFEDF SEGVSRSLDK TLGKLDVEMA KASTLLAGSV EQIGESVSEL
     DDVLSRVRA